Rabbit Monoclonal HMGA2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HMGA2.
Images
![Western blot - Anti-HMGA2 antibody [HL1936] (AB308208), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-hl1936-ab308208--western-blot-img441323.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HMGA2 antibody [HL1936] (AB308208)")
![Western blot - Anti-HMGA2 antibody [HL1936] (AB308208), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-hl1936-ab308208--western-blot-img407384.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HMGA2 antibody [HL1936] (AB308208)")
![Western blot - Anti-HMGA2 antibody [HL1936] (AB308208), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-hl1936-ab308208--western-blot-img407386.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HMGA2 antibody [HL1936] (AB308208)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [HL1936] (AB308208), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-hl1936-ab308208--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img407381.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [HL1936] (AB308208)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human HMGA2. The exact immunogen used to generate this antibody is proprietary information. Database link P52926
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Tested |
| Rat | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Target data
Function
Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Plays a role in postnatal myogenesis, is involved in satellite cell activation (By similarity). Positively regulates IGF2 expression through PLAG1 and in a PLAG1-independent manner (PubMed:28796236).
Alternative names
HMGIC, HMGA2, High mobility group protein HMGI-C, High mobility group AT-hook protein 2
Recommended products
Rabbit Monoclonal HMGA2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HMGA2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human HMGA2. The exact immunogen used to generate this antibody is proprietary information. Database link P52926
- Carrier free
- Yes
- Clone number
- HL1936
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-HMGA2 antibody [HL1936] (ab308208), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-hl1936-ab308208--western-blot-img441323.jpg "Western blot - Anti-HMGA2 antibody [HL1936] (ab308208)")
Western blot - Anti-HMGA2 antibody [HL1936] (ab308208)
Samples were seperated by 15% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
All lanes: Western blot - Anti-HMGA2 antibody [HL1936] (ab308208) at 1/500 dilution
Lane 1: 293T whole cell extracts at 30 µg
Lane 2: A431 whole cell extracts at 30 µg
Lane 3: HeLa whole cell extracts at 30 µg
Lane 4: HepG2 whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 11 kDa
![Western blot - Anti-HMGA2 antibody [HL1936] (ab308208), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-hl1936-ab308208--western-blot-img407384.jpg "Western blot - Anti-HMGA2 antibody [HL1936] (ab308208)")
Western blot - Anti-HMGA2 antibody [HL1936] (ab308208)
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [HL1936] (ab308208) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Lane 1: NIH-3T3 cell lysate at 30 µg
Lane 2: Raw264.7 cell lysate at 30 µg
Lane 3: C2C12 cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
![Western blot - Anti-HMGA2 antibody [HL1936] (ab308208), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-hl1936-ab308208--western-blot-img407386.jpg "Western blot - Anti-HMGA2 antibody [HL1936] (ab308208)")
Western blot - Anti-HMGA2 antibody [HL1936] (ab308208)
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [HL1936] (ab308208) diluted at 1/500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Rat2 whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [HL1936] (ab308208), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-hl1936-ab308208--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img407381.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [HL1936] (ab308208)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [HL1936] (ab308208)
Immunohistochemical analysis of Paraffin-embedded mouse stomach tissue labelling HMGA2 protein with ab308208 at 1/100 dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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