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AB67282

Anti-HMGB2 antibody

4

(4 Reviews)

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(40 Publications)

Anti-HMGB2 antibody (ab67282) is a rabbit polyclonal antibody detecting HMGB2 in Western Blot, IP. Suitable for Human, Mouse, Rat.

- Over 30 publications
- Trusted since 2008

View Alternative Names

HMG2, HMGB2, High mobility group protein B2, High mobility group protein 2, HMG-2

4 Images
Immunoprecipitation - Anti-HMGB2 antibody (AB67282)
  • IP

Unknown

Immunoprecipitation - Anti-HMGB2 antibody (AB67282)

Lanes
1 : 5 µg of Rabbit Anti-HMGB2 (ab67282) were used to immunoprecipitate HMGB2 from HEK-293T cell lysate
2 : Negative control – without cell lysate
3 : Negative control – without antibody
4 : Positive control – Extract of HEK-293T direct labeling

All lanes:

Immunoprecipitation - Anti-HMGB2 antibody (ab67282)

Predicted band size: 24 kDa

false

Immunocytochemistry - Anti-HMGB2 antibody (AB67282)
  • ICC

Unknown

Immunocytochemistry - Anti-HMGB2 antibody (AB67282)

Immunocytochemistry analysis of HUVEC cells using Anti-HMGB2 antibody (red) at a 1 : 50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).

Immunocytochemistry - Anti-HMGB2 antibody (AB67282)
  • ICC

Supplier Data

Immunocytochemistry - Anti-HMGB2 antibody (AB67282)

Nuclear localization of HMGB2 in PC12 cells.
A. Cells were stained with Rabbit Anti-HMGB2 (ab67282) at 1 µg/mL
B. DAPI staining
C. Merged staining of HMGB2 and DAPI indicating nuclear staining of HMGB2

Western blot - Anti-HMGB2 antibody (AB67282)
  • WB

Supplier Data

Western blot - Anti-HMGB2 antibody (AB67282)

All lanes:

Western blot - Anti-HMGB2 antibody (ab67282) at 2 mg/mL

Lane 1:

NIH3T3

Lane 2:

Rat1

Lane 3:

RINSH

Lane 4:

PC12

Lane 5:

K562

Lane 6:

HEK-293T

Lane 7:

HeLa nuclear

Lane 8:

HeLa

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC, IP, WB

applications

Immunogen

Synthetic Peptide within Human HMGB2 aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P26583

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Use 5-10µg.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-HMGB2 antibody (ab67282) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human, Mouse, Rat samples.

What is the molecular weight of HMGB2?
Anti-HMGB2 (ab67282) specifically detects a band for HMGB2 (UniProt: P26583) at a molecular weight of 24kDa.

Trusted by the scientific community
Anti-HMGB2 (ab67282) was first used in a scientific publication in 2008 and has been cited over 30 times in peer-reviewed journals.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. In the nucleus is an abundant chromatin-associated non-histone protein involved in transcription, chromatin remodeling and V(D)J recombination and probably other processes. Binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters by enhancing transcription factor binding and/or bringing distant regulatory sequences into close proximity (PubMed : 11909973, PubMed : 18413230, PubMed : 19522541, PubMed : 19965638, PubMed : 20123072, PubMed : 7797075). Involved in V(D)J recombination by acting as a cofactor of the RAG complex : acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS) (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a promiscuous immunogenic DNA/RNA sensor which cooperates with subsequent discriminative sensing by specific pattern recognition receptors (By similarity). In the extracellular compartment acts as a chemokine. Promotes proliferation and migration of endothelial cells implicating AGER/RAGE (PubMed : 19811285). Has antimicrobial activity in gastrointestinal epithelial tissues (PubMed : 23877675). Involved in inflammatory response to antigenic stimulus coupled with pro-inflammatory activity (By similarity). Involved in modulation of neurogenesis probably by regulation of neural stem proliferation (By similarity). Involved in articular cartilage surface maintenance implicating LEF1 and the Wnt/beta-catenin pathway (By similarity).
See full target information HMGB2
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Publications (40)

Recent publications for all applications. Explore the full list and refine your search

Nature 647:517-527 PubMed40866703

2025

Mechanical confinement governs phenotypic plasticity in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Miranda V Hunter,Eshita Joshi,Sydney Bowker,Emily Montal,Yilun Ma,Young Hun Kim,Zhifan Yang,Laura Tuffery,Zhuoning Li,Eric Rosiek,Alexander Browning,Reuben Moncada,Itai Yanai,Helen Byrne,Mara Monetti,Elisa de Stanchina,Pierre-Jacques Hamard,Richard P Koche,Richard M White

Stem cells (Dayton, Ohio) 43: PubMed40574692

2025

Single-cell analysis of heterogeneity and molecular changes in cultured corneal epithelial stem cells during serial passage.

Applications

Unspecified application

Species

Unspecified reactive species

Usanee Reinprayoon,Supaporn Khramchantuk,Natthida Kittimawikrom,Praewphan Ingrungruanglert,Sarinya Phodang,Thanakorn Jaemthaworn,Sira Sriswasdi,Nipan Israsena

Genome biology 26:100 PubMed40247387

2025

Hmgb2 improves astrocyte to neuron conversion by increasing the chromatin accessibility of genes associated with neuronal maturation in a proneuronal factor-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Priya Maddhesiya,Tjasa Lepko,Andrea Steiner-Mezzardi,Julia Schneider,Veronika Schwarz,Juliane Merl-Pham,Finja Berger,Stefanie M Hauck,Lorenza Ronfani,Marco Bianchi,Tatiana Simon,Anthodesmi Krontira,Giacomo Masserdotti,Magdalena Götz,Jovica Ninkovic

Nature neuroscience 28:457-469 PubMed39994409

2025

Astrocyte heterogeneity reveals region-specific astrogenesis in the white matter.

Applications

Unspecified application

Species

Unspecified reactive species

Riccardo Bocchi,Manja Thorwirth,Tatiana Simon-Ebert,Christina Koupourtidou,Solène Clavreul,Keegan Kolf,Patrizia Della Vecchia,Sara Bottes,Sebastian Jessberger,Jiafeng Zhou,Gulzar Wani,Gregor-Alexander Pilz,Jovica Ninkovic,Annalisa Buffo,Swetlana Sirko,Magdalena Götz,Judith Fischer-Sternjak

International journal of molecular sciences 25: PubMed38542079

2024

The Effect of HMGB1 and HMGB2 on Transcriptional Regulation Differs in Neuroendocrine and Adenocarcinoma Models of Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Martín Salamini-Montemurri,Ángel Vizoso-Vázquez,Aida Barreiro-Alonso,Lidia Lorenzo-Catoira,Esther Rodríguez-Belmonte,María-Esperanza Cerdán,Mónica Lamas-Maceiras

Aging cell 23:e14083 PubMed38196311

2024

Rapid and synchronous chemical induction of replicative-like senescence via a small molecule inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Spiros Palikyras,Konstantinos Sofiadis,Athanasia Stavropoulou,Adi Danieli-Mackay,Vassiliki Varamogianni-Mamatsi,David Hörl,Simona Nasiscionyte,Yajie Zhu,Ioanna Papadionysiou,Antonis Papadakis,Natasa Josipovic,Anne Zirkel,Aoife O'Connell,Gary Loughran,James Keane,Audrey Michel,Wolfgang Wagner,Andreas Beyer,Hartmann Harz,Heinrich Leonhardt,Grazvydas Lukinavicius,Christoforos Nikolaou,Argyris Papantonis

Nature 616:814-821 PubMed37046086

2023

Ageing-associated changes in transcriptional elongation influence longevity.

Applications

Unspecified application

Species

Unspecified reactive species

Cédric Debès,Antonios Papadakis,Sebastian Grönke,Özlem Karalay,Luke S Tain,Athanasia Mizi,Shuhei Nakamura,Oliver Hahn,Carina Weigelt,Natasa Josipovic,Anne Zirkel,Isabell Brusius,Konstantinos Sofiadis,Mantha Lamprousi,Yu-Xuan Lu,Wenming Huang,Reza Esmaillie,Torsten Kubacki,Martin R Späth,Bernhard Schermer,Thomas Benzing,Roman-Ulrich Müller,Adam Antebi,Linda Partridge,Argyris Papantonis,Andreas Beyer

Journal of biochemical and molecular toxicology 37:e23306 PubMed36935520

2023

Circular RNA SLTM as a miR-421-competing endogenous RNA to mediate HMGB2 expression stimulates apoptosis and inflammation in arthritic chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Zhang,XiaoBing Xiang,BenGen Zhou,JianFa Chen,YouQiang Sun,ShuangXiao Zhang,AiHua Li,Jie Li

Cell death & disease 13:912 PubMed36309482

2022

Bromodomain-containing protein 4 (BRD4) as an epigenetic regulator of fatty acid metabolism genes and ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Minghua Yang,Ke Liu,Pan Chen,Hongyi Zhu,Junjie Wang,Jun Huang

Genomics, proteomics & bioinformatics 19:423-436 PubMed34775075

2021

Single-cell RNA Sequencing Reveals Thoracolumbar Vertebra Heterogeneity and Rib-genesis in Pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Jianbo Li,Ligang Wang,Dawei Yu,Junfeng Hao,Longchao Zhang,Adeniyi C Adeola,Bingyu Mao,Yun Gao,Shifang Wu,Chunling Zhu,Yongqing Zhang,Jilong Ren,Changgai Mu,David M Irwin,Lixian Wang,Tang Hai,Haibing Xie,Yaping Zhang
View all publications
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