Rabbit Recombinant Monoclonal HMGB2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-HMGB2 antibody [EPR6302] - BSA and Azide free (AB248543), expandable thumbnail](https://content.abcam.com/products/images/hmgb2-antibody-epr6302-bsa-and-azide-free-ab248543--western-blot-img55233.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HMGB2 antibody [EPR6302] - BSA and Azide free (AB248543)")
![Western blot - Anti-HMGB2 antibody [EPR6302] - BSA and Azide free (AB248543), expandable thumbnail](https://content.abcam.com/products/images/hmgb2-antibody-epr6302-bsa-and-azide-free-ab248543--western-blot-img161315.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HMGB2 antibody [EPR6302] - BSA and Azide free (AB248543)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Expected |
| Mouse | Not recommended | Not recommended | Predicted | Expected |
| Rat | Not recommended | Not recommended | Predicted | Expected |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target data
Function
Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. In the nucleus is an abundant chromatin-associated non-histone protein involved in transcription, chromatin remodeling and V(D)J recombination and probably other processes. Binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters by enhancing transcription factor binding and/or bringing distant regulatory sequences into close proximity (PubMed:11909973, PubMed:18413230, PubMed:19522541, PubMed:19965638, PubMed:20123072, PubMed:7797075). Involved in V(D)J recombination by acting as a cofactor of the RAG complex: acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS) (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a promiscuous immunogenic DNA/RNA sensor which cooperates with subsequent discriminative sensing by specific pattern recognition receptors (By similarity). In the extracellular compartment acts as a chemokine. Promotes proliferation and migration of endothelial cells implicating AGER/RAGE (PubMed:19811285). Has antimicrobial activity in gastrointestinal epithelial tissues (PubMed:23877675). Involved in inflammatory response to antigenic stimulus coupled with pro-inflammatory activity (By similarity). Involved in modulation of neurogenesis probably by regulation of neural stem proliferation (By similarity). Involved in articular cartilage surface maintenance implicating LEF1 and the Wnt/beta-catenin pathway (By similarity).
Alternative names
HMG2, HMGB2, High mobility group protein B2, High mobility group protein 2, HMG-2
Recommended products
Rabbit Recombinant Monoclonal HMGB2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6302
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 2.97 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248543 is the carrier-free version of Anti-HMGB2 antibody [EPR6302] ab133540.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-HMGB2 antibody [EPR6302] - BSA and Azide free (ab248543), expandable thumbnail](https://content.abcam.com/products/images/hmgb2-antibody-epr6302-bsa-and-azide-free-ab248543--western-blot-img55233.jpg "Western blot - Anti-HMGB2 antibody [EPR6302] - BSA and Azide free (ab248543)")
Western blot - Anti-HMGB2 antibody [EPR6302] - BSA and Azide free (ab248543)
This data was developed using the same antibody clone in a different buffer formulation (Anti-HMGB2 antibody [EPR6302] ab133540).
Lanes 1- 2: Merged signal (red and green). Green - Anti-HMGB2 antibody [EPR6302] ab133540 observed at 24 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-HMGB2 antibody [EPR6302] ab133540 was shown to react with HMGB2 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line Human HMGB2 knockout HEK-293T cell line ab266358 (knockout cell lysate Human HMGB2 knockout HEK-293T cell lysate ab257156) was used. Wild-type HEK-293T and HMGB2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-HMGB2 antibody [EPR6302] ab133540 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-HMGB2 antibody [EPR6302] (Anti-HMGB2 antibody [EPR6302] ab133540) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: HMGB2 knockout HEK-293T cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDa
![Western blot - Anti-HMGB2 antibody [EPR6302] - BSA and Azide free (ab248543), expandable thumbnail](https://content.abcam.com/products/images/hmgb2-antibody-epr6302-bsa-and-azide-free-ab248543--western-blot-img161315.jpg "Western blot - Anti-HMGB2 antibody [EPR6302] - BSA and Azide free (ab248543)")
Western blot - Anti-HMGB2 antibody [EPR6302] - BSA and Azide free (ab248543)
This data was developed using the same antibody clone in a different buffer formulation (Anti-HMGB2 antibody [EPR6302] ab133540).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-HMGB2 antibody [EPR6302] ab133540 observed at 24 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-HMGB2 antibody [EPR6302] ab133540 was shown to recognize HMGB2 in wild-type HAP1 cells as signal was lost at the expected MW in HMGB2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and HMGB2 knockout samples were subjected to SDS-PAGE. Anti-HMGB2 antibody [EPR6302] ab133540 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-HMGB2 antibody [EPR6302] - BSA and Azide free (ab248543) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: HMGB2 knockout HAP1 whole cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: K562 cell lysate at 20 µg
Predicted band size: 24 kDa
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