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Rabbit Polyclonal HMGCL antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HMGCL aa 1-200.

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Images

Western blot - Anti-HMGCL antibody (AB97293), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-HMGCL antibody (AB97293), expandable thumbnail

Publications

  • Nature communications 11:31482020
    Leucine regulates autophagy via acetylation of the mTORC1 component raptor.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Sung Min Son et. al.
    PubMed 32561715

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human HMGCL aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P35914

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/200

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Mitochondrial 3-hydroxymethyl-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis. Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism.

Alternative names

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Rabbit Polyclonal HMGCL antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HMGCL aa 1-200.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human HMGCL aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P35914
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

Purified by antigen affinity chromatography.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Activity summary

HMGCL or 3-hydroxymethyl-3-methylglutaryl-CoA lyase plays an important role in ketogenesis and leucine catabolism. This enzyme catalyzes the cleavage of HMG-CoA to acetoacetate and acetyl-CoA. It has a molecular mass of approximately 33 kDa. HMGCL expresses mainly in the liver kidney and brain where ketogenesis is active signifying its essential role in energy metabolism in fasting conditions.

Biological function summary

This enzyme contributes to the production of ketone bodies important components during periods of low glucose availability. HMGCL does not function as part of a multi-protein complex but its action underpins the metabolic switch from glucose to fatty acids and ketone body usage. Proper functioning of this enzyme helps maintain energy balance especially during fasting and extended exercise.

Pathways

HMGCL plays a significant role in the ketogenic pathway linking fatty acid oxidation to ketone body production. It also intersects with leucine degradation pathways reflecting its dual substrate specificity. HMGCL shares links with proteins involved in lipid metabolism such as HMG-CoA synthase which provides the substrate for its enzymatic activity.

Associated diseases and disorders

Defects in HMGCL associate with HMG-CoA lyase deficiency a metabolic disorder hallmarked by hypoketotic hypoglycemia and metabolic acidosis. This condition highlights the enzyme's role in ketogenesis. Additionally its impaired function links to potential exacerbation of seizures due to energy metabolism failure. The enzyme's activity correlates with enzymes like carnitine palmitoyltransferase I which also impact energy metabolism through ketogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • Western blot - Anti-HMGCL antibody (ab97293), expandable thumbnail

    Western blot - Anti-HMGCL antibody (ab97293)

    10% SDS PAGE

    All lanes: Western blot - Anti-HMGCL antibody (ab97293) at 1/3000 dilution

    All lanes: H1299 whole cell lysate at 30 µg

    Predicted band size: 34 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-HMGCL antibody (ab97293), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HMGCL antibody (ab97293)

    ab97293, at a 1/200 dilution, staining HMGCL in paraformaldehyde fixed HeLa by Immunofluorescence analysis.
    The lower image was merged with DNA probe.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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