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AB97293

Anti-HMGCL antibody

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(2 Publications)

Rabbit Polyclonal HMGCL antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HMGCL aa 1-200.

View Alternative Names

HL, HMG-CoA lyase, 3-hydroxy-3-methylglutarate-CoA lyase, HMGCL

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-HMGCL antibody (AB97293)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HMGCL antibody (AB97293)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling HMGCL protein at mitochondria using ab97293 at a 1/500 dilution (Green).
Hoechst 33342 was used for nuclear staining (Blue). Scale bar = 10 µm

Western blot - Anti-HMGCL antibody (AB97293)
  • WB

Unknown

Western blot - Anti-HMGCL antibody (AB97293)

10% SDS PAGE

All lanes:

Western blot - Anti-HMGCL antibody (ab97293) at 1/3000 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 34 kDa

false

Western blot - Anti-HMGCL antibody (AB97293)
  • WB

Supplier Data

Western blot - Anti-HMGCL antibody (AB97293)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-HMGCL antibody (ab97293) at 1/10000 dilution

All lanes:

Mouse liver lysate at 50 µg

Predicted band size: 34 kDa

false

Western blot - Anti-HMGCL antibody (AB97293)
  • WB

Supplier Data

Western blot - Anti-HMGCL antibody (AB97293)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-HMGCL antibody (ab97293) at 1/10000 dilution

All lanes:

Rat liver lysate at 50 µg

Predicted band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human HMGCL aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P35914

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCL antibody [EPR15551] - BSA and Azide free (AB232604)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified by antigen affinity chromatography.
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HMGCL or 3-hydroxymethyl-3-methylglutaryl-CoA lyase plays an important role in ketogenesis and leucine catabolism. This enzyme catalyzes the cleavage of HMG-CoA to acetoacetate and acetyl-CoA. It has a molecular mass of approximately 33 kDa. HMGCL expresses mainly in the liver kidney and brain where ketogenesis is active signifying its essential role in energy metabolism in fasting conditions.
Biological function summary

This enzyme contributes to the production of ketone bodies important components during periods of low glucose availability. HMGCL does not function as part of a multi-protein complex but its action underpins the metabolic switch from glucose to fatty acids and ketone body usage. Proper functioning of this enzyme helps maintain energy balance especially during fasting and extended exercise.

Pathways

HMGCL plays a significant role in the ketogenic pathway linking fatty acid oxidation to ketone body production. It also intersects with leucine degradation pathways reflecting its dual substrate specificity. HMGCL shares links with proteins involved in lipid metabolism such as HMG-CoA synthase which provides the substrate for its enzymatic activity.

Defects in HMGCL associate with HMG-CoA lyase deficiency a metabolic disorder hallmarked by hypoketotic hypoglycemia and metabolic acidosis. This condition highlights the enzyme's role in ketogenesis. Additionally its impaired function links to potential exacerbation of seizures due to energy metabolism failure. The enzyme's activity correlates with enzymes like carnitine palmitoyltransferase I which also impact energy metabolism through ketogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial 3-hydroxy-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis. Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism.
See full target information HMGCL
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 11:3148 PubMed32561715

2020

Leucine regulates autophagy via acetylation of the mTORC1 component raptor.

Applications

Unspecified application

Species

Unspecified reactive species

Sung Min Son,So Jung Park,Eleanna Stamatakou,Mariella Vicinanza,Fiona M Menzies,David C Rubinsztein

Oxidative medicine and cellular longevity 2020:7126976 PubMed32256960

2020

Chidamide Inhibits Glioma Cells by Increasing Oxidative Stress via the miRNA-338-5p Regulation of Hedgehog Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Haixia Zhou,Liang Han,Han Wang,Jun Wei,Zhigang Guo,Zhaohui Li
View all publications
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Product promise

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