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Rabbit Recombinant Monoclonal HMGCL antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-HMGCL antibody [EPR15551] (AB197022), expandable thumbnail
  • Western blot - Anti-HMGCL antibody [EPR15551] (AB197022), expandable thumbnail
  • Western blot - Anti-HMGCL antibody [EPR15551] (AB197022), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCL antibody [EPR15551] (AB197022), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Mitochondrial 3-hydroxymethyl-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis. Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism.

Alternative names

HL, HMG-CoA lyase, 3-hydroxy-3-methylglutarate-CoA lyase, HMGCL

Recommended products

Rabbit Recombinant Monoclonal HMGCL antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15551
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

HMGCL or 3-hydroxymethyl-3-methylglutaryl-CoA lyase plays an important role in ketogenesis and leucine catabolism. This enzyme catalyzes the cleavage of HMG-CoA to acetoacetate and acetyl-CoA. It has a molecular mass of approximately 33 kDa. HMGCL expresses mainly in the liver kidney and brain where ketogenesis is active signifying its essential role in energy metabolism in fasting conditions.

Biological function summary

This enzyme contributes to the production of ketone bodies important components during periods of low glucose availability. HMGCL does not function as part of a multi-protein complex but its action underpins the metabolic switch from glucose to fatty acids and ketone body usage. Proper functioning of this enzyme helps maintain energy balance especially during fasting and extended exercise.

Pathways

HMGCL plays a significant role in the ketogenic pathway linking fatty acid oxidation to ketone body production. It also intersects with leucine degradation pathways reflecting its dual substrate specificity. HMGCL shares links with proteins involved in lipid metabolism such as HMG-CoA synthase which provides the substrate for its enzymatic activity.

Associated diseases and disorders

Defects in HMGCL associate with HMG-CoA lyase deficiency a metabolic disorder hallmarked by hypoketotic hypoglycemia and metabolic acidosis. This condition highlights the enzyme's role in ketogenesis. Additionally its impaired function links to potential exacerbation of seizures due to energy metabolism failure. The enzyme's activity correlates with enzymes like carnitine palmitoyltransferase I which also impact energy metabolism through ketogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-HMGCL antibody [EPR15551] (ab197022), expandable thumbnail

    Western blot - Anti-HMGCL antibody [EPR15551] (ab197022)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-HMGCL antibody [EPR15551] (ab197022) at 1/1000 dilution

    All lanes: 293 (Human epithelial cells from embryonic kidney) cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa

    Exposure time: 3min

  • Western blot - Anti-HMGCL antibody [EPR15551] (ab197022), expandable thumbnail

    Western blot - Anti-HMGCL antibody [EPR15551] (ab197022)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-HMGCL antibody [EPR15551] (ab197022) at 1/5000 dilution

    All lanes: Human testis lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa

    Exposure time: 1min

  • Western blot - Anti-HMGCL antibody [EPR15551] (ab197022), expandable thumbnail

    Western blot - Anti-HMGCL antibody [EPR15551] (ab197022)

    Blocking/dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-HMGCL antibody [EPR15551] (ab197022) at 1/10000 dilution

    All lanes: A431 (Human epidermoid carcinoma) cell lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCL antibody [EPR15551] (ab197022), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCL antibody [EPR15551] (ab197022)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling HMGCL with ab197022 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Counter stained with hematoxylin.
    Inset image: negative control obtained using PBS instead of ab197022 and secondary antibody only.
    Note: Cytoplasm staining on human liver tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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