AB101576

Anti-HMGCLL1 antibody

Name: Anti-HMGCLL1 antibody (ab101576) | Abcam
Brand: Abcam Limited
SKU: AB101576
Price: 495 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal HMGCLL1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HMGCLL1 aa 100-350.

View Alternative Names

3-hydroxy-3-methylglutaryl-CoA lyase-like protein 1, Endoplasmic reticulum 3-hydroxy-3-methylglutaryl-CoA lyase, HMGCL-like 1, er-cHL, HMGCLL1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCLL1 antibody (AB101576)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCLL1 antibody (AB101576)

ab101576, at 1/100 dilution, staining HMGCLL1 in formalin-fixed, paraffin-embedded Human fetal brain tissue by Immunohistochemistry.

Unknown

Western blot - Anti-HMGCLL1 antibody (AB101576)

All lanes:

Western blot - Anti-HMGCLL1 antibody (ab101576) at 1/500 dilution

All lanes:

Human fetal liver lysate

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human HMGCLL1 aa 100-350. The exact immunogen used to generate this antibody is proprietary information.

Q8TB92

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

Reconstitute in 200 µL of water

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HMGCLL1 protein also known as 3-Hydroxymethyl-3-methylglutaryl-CoA lyase-like 1 is an enzyme that participates in specific biochemical processes. Its molecular mass is approximately 34 kDa. HMGCLL1 is mainly expressed in the liver and to a lesser extent in other tissues like the kidney. This expression pattern indicates a potential role in metabolic processes specific to these organs. Its mechanical function involves enzymatic activity related to CoA derivatives.

Biological function summary

The functions of HMGCLL1 relate to energy metabolism and lipogenesis. It participates similarly to its more studied counterpart the HMG-CoA lyase in catalyzing reactions critical for ketogenesis and cholesterol synthesis. Unlike HMG-CoA lyase HMGCLL1 is not known to form part of any larger enzymatic complex. However its role still complements pathways involved in essential fatty acid conversion and ketone body formation.

Pathways

HMGCLL1 plays a role in lipid metabolism and ketogenesis pathways. These pathways are integral for the breakdown and conversion of fatty acids into energy especially in fasting states. In the ketogenesis pathway its activity aligns with similar enzymes like HMG-CoA reductase which facilitates the production of ketone bodies. In lipid metabolism it shares pathway mechanics with related proteins like HMGCR though more specific roles of HMGCLL1 continue to be explored.

Research suggests that HMGCLL1 could be associated with metabolic conditions such as non-alcoholic fatty liver disease (NAFLD) and potentially with some cholesterol-related disorders. In NAFLD altered expression of proteins involved in lipid metabolism including HMGCLL1 might contribute to disease development or progression. Associations with cholesterol-related disorders also connect HMGCLL1 to proteins like HMGCR due to their shared roles in mevalonate and cholesterol biosynthesis pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-mitochondrial 3-hydroxymethyl-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis, the products of which support energy production in nonhepatic animal tissues.

See full target information HMGCLL1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 41:e110466 PubMed35307861

2022

Ketogenic HMG-CoA lyase and its product β-hydroxybutyrate promote pancreatic cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Victoire Gouirand,Tristan Gicquel,Evan C Lien,Emilie Jaune-Pons,Quentin Da Costa,Pascal Finetti,Elodie Metay,Camille Duluc,Jared R Mayers,Stephane Audebert,Luc Camoin,Laurence Borge,Marion Rubis,Julie Leca,Jeremy Nigri,François Bertucci,Nelson Dusetti,Juan L Iovanna,Richard Tomasini,Ghislain Bidaut,Fabienne Guillaumond,Matthew G Vander Heiden,Sophie Vasseur

Journal of Alzheimer's disease : JAD 78:1743-1753 PubMed33285636

2020

OGA Inhibition Alters Energetics and Nutrient Sensing in Alzheimer's Disease Cytoplasmic Hybrids.

Applications

Unspecified application

Species

Unspecified reactive species

Jarrod Flax,Heather M Wilkins,Reegan Miller,Sarah Griffith,Gentry K Cork,Amy Qiang,Jeffrey Thompson,Russell H Swerdlow,Chad Slawson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HMGCLL1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCLL1 antibody (AB101576)

Western blot - Anti-HMGCLL1 antibody (AB101576)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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