AB327101

Anti-HMGCR Antibody [CL0259]

Name: Anti-HMGCR Antibody [CL0259] (ab327101) | Abcam
Brand: Abcam Limited
SKU: AB327101
Price: 580 USD
Availability: InStock

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Mouse Monoclonal HMGCR antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human 3-hydroxy-3-methylglutaryl-coenzyme A reductase.

View Alternative Names

3-hydroxy-3-methylglutaryl-coenzyme A reductase, HMG-CoA reductase, HMGCR

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR Antibody [CL0259] (AB327101)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR Antibody [CL0259] (AB327101)

Immunohistochemical staining of human breast cancer using ab327101 at 1 : 20–1 : 50 dilution, showing moderate cytoplasmic positivity in tumor cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR Antibody [CL0259] (AB327101)

Immunohistochemical staining of human liver cancer using ab327101 at 1 : 20–1 : 50 dilution, showing strong cytoplasmic positivity in tumor cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR Antibody [CL0259] (AB327101)

Immunohistochemical staining of human liver using ab327101 at 1 : 20–1 : 50 dilution, showing moderate cytoplasmic positivity in hepatocytes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR Antibody [CL0259] (AB327101)

Immunohistochemistry analysis in human liver and skeletal muscle tissues using ab327101 at 1 : 20–1 : 50 dilution. Corresponding HMGCR RNA-seq data are presented for the same tissues.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR Antibody [CL0259] (AB327101)

Immunohistochemical staining of human fallopian tube using ab327101 at 1 : 20–1 : 50 dilution, showing moderate to strong cytoplasmic positivity in glandular cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR Antibody [CL0259] (AB327101)

Immunohistochemical staining of human uterine cervix using ab327101 at 1 : 20–1 : 50 dilution, showing moderate cytoplasmic positivity in squamous epithelial cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR Antibody [CL0259] (AB327101)

Immunohistochemical staining of human skeletal muscle using ab327101 at 1 : 20–1 : 50 dilution, showing no positivity in myocytes as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0259

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human 3-hydroxy-3-methylglutaryl-coenzyme A reductase.

P04035

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis (PubMed : 21357570, PubMed : 2991281, PubMed : 36745799, PubMed : 6995544). HMGCR is the main target of statins, a class of cholesterol-lowering drugs (PubMed : 11349148, PubMed : 18540668, PubMed : 36745799).

See full target information 3-hydroxy-3-methylglutaryl-coenzyme A reductase

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Product promise

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