AB242315

Anti-HMGCR antibody [CL0260]

Name: Anti-HMGCR antibody [CL0260] (ab242315) | Abcam
Brand: Abcam Limited
SKU: AB242315
Price: 440 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(28 Publications)

Anti-HMGCR antibody [CL0260] (ab242315) is a mouse monoclonal antibody detecting HMGCR in Western Blot, IHC-P. Suitable for Human.

- Over 10 publications

View Alternative Names

3-hydroxy-3-methylglutaryl-coenzyme A reductase, HMG-CoA reductase, HMGCR

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR antibody [CL0260] (AB242315)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR antibody [CL0260] (AB242315)

Paraffin-embedded human liver tissue stained for HMGCR using ab242315 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR antibody [CL0260] (AB242315)

Negative Control :

Paraffin-embedded human skeletal muscle tissue stained for HMGCR using ab242315 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR antibody [CL0260] (AB242315)

Paraffin-embedded human liver cancer tissue stained for HMGCR using ab242315 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR antibody [CL0260] (AB242315)

Paraffin-embedded human testis tissue stained for HMGCR using ab242315 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR antibody [CL0260] (AB242315)

Paraffin-embedded human breast cancer tissue stained for HMGCR using ab242315 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR antibody [CL0260] (AB242315)

Paraffin-embedded human fallopian tube tissue stained for HMGCR using ab242315 at 1/50 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-HMGCR antibody [CL0260] (AB242315)

All lanes:

Western blot - Anti-HMGCR antibody [CL0260] (ab242315) at 1 µg/mL

Lane 1:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Predicted band size: 97 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0260

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human 3-hydroxy-3-methylglutaryl-coenzyme A reductase aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P04035

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-HMGCR antibody [CL0260] (ab242315) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

Trusted by the scientific community
Anti-HMGCR [CL0260] (ab242315) was first used in a scientific publication in 2018 and has been cited over 10 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from TCS.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HMG-CoA reductase also known as HMGCR plays an important role in cholesterol biosynthesis. This enzyme catalyzes the conversion of HMG-CoA (3-hydroxy-3-methyl-glutaryl-coenzyme A) to mevalonate a critical step in the isoprenoid synthesis pathway. HMGCR is a membrane-bound enzyme located primarily in the liver on the endoplasmic reticulum. The enzyme has a molecular mass of approximately 97 kDa. Due to its central role in cholesterol metabolism HMGCR has become a primary target for statins such as pravastatin and simvastatin.

Biological function summary

HMGCR reduces HMG-CoA to mevalonate an important precursor for several important biomolecules including cholesterol. It exists as part of a complex regulatory system impacted by intracellular cholesterol levels. The activity of HMGCR is tightly controlled through feedback inhibition and post-translational modifications. The enzyme interacts with other proteins in the endoplasmic reticulum as part of the cholesterol homeostatic mechanism in cells expressing it including hepatocytes.

Pathways

HMGCR is an essential component of the mevalonate pathway which is important for the synthesis of sterols and non-sterol isoprenoids. This pathway also intersects with the biosynthesis of ubiquinone and dolichol. The regulation of cholesterol synthesis involves the coordinated activity of HMG-CoA reductase and LDL receptor proteins. Their interplay ensures a balanced cholesterol level and integrates with the response to cellular demands and dietary intake.

HMGCR is strongly linked to cardiovascular diseases due to its influential role in cholesterol synthesis. Inhibitors like pravastatin and simvastatin effectively lower blood cholesterol levels and reduce the risk of coronary artery disease by targeting HMGCR. The Statins also have potential associations with muscular disorders because of shared pathways involving proteins such as SREBP (sterol regulatory element-binding proteins) which regulate the transcription of genes involved in lipid metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis (PubMed : 21357570, PubMed : 2991281, PubMed : 36745799, PubMed : 6995544). HMGCR is the main target of statins, a class of cholesterol-lowering drugs (PubMed : 11349148, PubMed : 18540668, PubMed : 36745799).

See full target information 3-hydroxy-3-methylglutaryl-coenzyme A reductase

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed40923320

2025

Lomitapide enhances cytotoxic effects of temozolomide in chemotherapy-resistant glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Alyona Ivanova,Taylor M Wilson,Kimia Ghannad-Zadeh,Esmond Tse,Robert Flick,Megan Wu,Sunit Das

Frontiers in immunology 16:1592597 PubMed40735328

2025

Revealing the impact of quorum sensing molecule 2'-aminoacetophenone on the human bronchial-airway epithelium and pulmonary endothelium using a human airway-on-a-chip.

Applications

Unspecified application

Species

Unspecified reactive species

Shifu Aggarwal,Arijit Chakraborty,Vijay K Singh,Stephen Lory,Katia Karalis,Laurence G Rahme

Cell death discovery 11:245 PubMed40399275

2025

miR-139-5p activates ferroptosis by inhibiting the expression of HMG-CoA reductase to inhibit the progression of glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongsheng You,Fei Wu,Yaofeng Zheng,Hongling Yang,Jianbo Ye,Hongyi Cai,Chuangcai Luo,Yang Liu,Yiquan Ke,Xiangdong Xu

Scientific reports 15:15051 PubMed40301465

2025

ApoE4 requires lipidation enhancement to resolve cellular lipid and protein abnormalities following NPC1 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Erika Di Biase,Kyle J Connolly,Ingrid Crumpton,Oliver Cooper,Penelope J Hallett,Ole Isacson

International journal of chronic obstructive pulmonary disease 20:411-423 PubMed40008109

2025

Cholesterol Accumulation Enhances Cigarette Smoke-Induced Airway Epithelial Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Du Jing,Jin-Kang Yu,Hai-Pin Chen,Ling-Ling Dong,Wen Li,Zhou-Yang Li,Jie-Sen Zhou

Experimental and therapeutic medicine 29:46 PubMed39885913

2025

Lindl. alkaloids improve lipid metabolism by increasing LDL uptake through regulation of the LXRα/IDOL/LDLR pathway and inhibition of PCSK9 expression in HepG2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Sun,Hao-Rui Liu,Ya-Xin Zhu,Wei Zhang,Jing-Shan Shi,Qin Wu,Rui-Xia Xu

Scientific reports 14:30157 PubMed39627481

2024

Differentiating erythroblasts adapt to mechanical stimulation by upregulation of cholesterol biosynthesis via S1P/SREBP-induced HMGCR expression.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Iacono,Asena Abay,Joan S Gallego Murillo,Francesca Aglialoro,Nurcan Yagci,Eszter Varga,Tieme Bijlsma,Justine Sohler,Kerly Fu,Julie A Reisz,Amy Argabright,Angelo D'Alessandro,Arthur F Svendsen,Marieke von Lindern,Emile van den Akker

Molecular cell 84:2166-2184.e9 PubMed38788716

2024

mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sang Ah Yi,Sara Sepic,Brenda A Schulman,Alban Ordureau,Heeseon An

Molecular medicine reports 29: PubMed38299233

2024

Activation of AMPK pathway by low‑dose donafenib and atorvastatin combination improves high‑fat diet‑induced metabolic dysfunction‑associated steatotic liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yaowei Bai,Kequan Chen,Jiacheng Liu,Yingliang Wang,Chaoyang Wang,Shuguang Ju,Chen Zhou,Wei Yao,Bin Xiong,Chuansheng Zheng

Frontiers in molecular neuroscience 16:1237458 PubMed37900943

2023

Cholinergic neurodegeneration and cholesterol metabolism dysregulation by constitutive p75 signaling in the p75-KO mice.

Applications

Unspecified application

Species

Unspecified reactive species

Raquel Comaposada-Baró,Andrea Benito-Martínez,Juan Julian Escribano-Saiz,María Luisa Franco,Lorenzo Ceccarelli,Isabel Calatayud-Baselga,Helena Mira,Marçal Vilar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-HMGCR antibody [CL0260]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR antibody [CL0260] (AB242315)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR antibody [CL0260] (AB242315)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR antibody [CL0260] (AB242315)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR antibody [CL0260] (AB242315)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR antibody [CL0260] (AB242315)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR antibody [CL0260] (AB242315)

Western blot - Anti-HMGCR antibody [CL0260] (AB242315)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (28)

Product promise