AB137043

Anti-HMGCS2 antibody [EPR8642]

Name: Anti-HMGCS2 antibody [EPR8642] (ab137043) | Abcam
Brand: Abcam Limited
SKU: AB137043
Price: 215 USD
Availability: InStock
Rating: 5 (7 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(7 Reviews)

(46 Publications)

Anti-HMGCS2 antibody [EPR8642] (ab137043) is a rabbit monoclonal antibody detecting HMGCS2 in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications

View Alternative Names

HMG-CoA synthase, 3-hydroxy-3-methylglutaryl coenzyme A synthase, HMGCS2

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (AB137043)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (AB137043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling HMGCS2 with unpurified ab137043 at a dilution of 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (AB137043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling HMGCS2 with purified ab137043 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (AB137043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling HMGCS2 with unpurified ab137043 at a diltuion of 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (AB137043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling HMGCS2 with purified ab137043 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (AB137043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling HMGCS2 with purified ab137043 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Lab

Western blot - Anti-HMGCS2 antibody [EPR8642] (AB137043)

Blocking and dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/5000 dilution

Lane 1:

Caco-2 whole cell lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

Predicted band size: 57 kDa

Observed band size: 50 kDa

false

Lab

Western blot - Anti-HMGCS2 antibody [EPR8642] (AB137043)

Blocking and dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/10000 dilution

All lanes:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

Predicted band size: 57 kDa

Observed band size: 50 kDa

false

Lab

Western blot - Anti-HMGCS2 antibody [EPR8642] (AB137043)

Blocking and dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/1000 dilution

All lanes:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

Predicted band size: 57 kDa

Observed band size: 50 kDa

false

Unknown

Western blot - Anti-HMGCS2 antibody [EPR8642] (AB137043)

All lanes:

Western blot - Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

Caco 2 lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 57 kDa

false

Carrier free

Anti-HMGCS2 antibody [EPR8642] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-HMGCS2 antibody [EPR8642]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-HMGCS2 antibody [EPR8642]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8642

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Does not cross react with HMGCS1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/1000", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/1000", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/1000", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-HMGCS2 antibody [EPR8642] (ab137043) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of HMGCS2?
Anti-HMGCS2 [EPR8642] (ab137043) specifically detects a band for HMGCS2 (UniProt: P54868) at a molecular weight of 57kDa.

Trusted by the scientific community
Anti-HMGCS2 [EPR8642] (ab137043) was first used in a scientific publication in 2012 and has been cited over 30 times in peer-reviewed journals.

Reviewed by scientists
Anti-HMGCS2 [EPR8642] (ab137043) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR8642] also available for your convenience: ab137043, Carrier free - ab240099, Alexa Fluor® 488 - ab309164, Alexa Fluor® 647 - ab309165

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HMGCS2 also known as hydroxymethylglutaryl-CoA synthase 2 is an enzyme involved in ketogenesis. It catalyzes the conversion of acetyl-CoA to HMG-CoA an essential step in the production of ketone bodies. This enzyme has a molecular mass of approximately 57 kDa. HMGCS2 is mainly expressed in the liver and is located within the mitochondria where it works under controlled metabolic conditions.

Biological function summary

HMGCS2 activates the ketogenesis process which generates ketone bodies such as acetoacetate and Β-hydroxybutyrate that serve as alternative energy sources when glucose is scarce. This enzyme is not known to be part of any larger protein complex. During fasting states or prolonged exercise the liver uptakes fatty acids and converts them into ketone bodies through HMGCS2 activity providing energy especially for organs like the brain and muscles.

Pathways

HMGCS2 functions in the ketogenesis and lipid metabolism pathways. It works alongside enzymes like HMG-CoA lyase which further processes HMG-CoA into acetoacetate a precursor for other ketone bodies. The interplay of HMGCS2 with fatty acid oxidation allows it to regulate energy homeostasis coordinating with enzymes such as carnitine palmitoyltransferase I which aids in transporting fatty acids into mitochondria for oxidation.

HMGCS2 malfunctions or deficiencies relate to disorders such as hypoketotic hypoglycemia and hepatic encephalopathy. Disrupted activity can affect energy balance particularly during fasting impacting glucose homeostasis. Alterations in HMGCS2 expression or function can connect with proteins like medium-chain acyl-CoA dehydrogenase which plays roles in fatty acid metabolism disorders further complicating the metabolic landscape when HMGCS2 is impaired.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the first irreversible step in ketogenesis, condensing acetyl-CoA to acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate.

See full target information Hydroxymethylglutaryl-CoA synthase, mitochondrial

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Publications (46)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0318178 PubMed40338840

2025

Fatty acid metabolism suppresses neonatal cardiomyocyte proliferation by increasing PDK4 and HMGCS2 expression through PPARδ.

Applications

Unspecified application

Species

Unspecified reactive species

Shota Tanaka,Akane Hirota,Yoshiaki Okada,Masanori Obana,Yasushi Fujio

American journal of physiology. Gastrointestinal and liver physiology 327:G877-G899 PubMed39404772

2024

Alterations in cellular metabolic pathway and epithelial cell maturation induced by MYO5B defects are partially reversible by LPAR5 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Momoh,Sudiksha Rathan-Kumar,Andreanna Burman,Monica E Brown,Francisca Adeniran,Cynthia Ramos,James R Goldenring,Joseph T Roland,Izumi Kaji

Proceedings of the National Academy of Sciences of the United States of America 121:e2405001121 PubMed39361648

2024

Enhancer landscape of lung neuroendocrine tumors reveals regulatory and developmental signatures with potential theranostic implications.

Applications

Unspecified application

Species

Unspecified reactive species

Ester Davis,Shani Avniel-Polak,Shahd Abu-Kamel,Israel Antman,Tsipora Saadoun,Chava Brim,Mohammad Jumaa,Yariv Maron,Ofra Maimon,Anat Bel-Ange,Karine Atlan,Tomer Tzur,Firas Abu Akar,Ori Wald,Uzi Izhar,Merav Hecht,Simona Grozinsky-Glasberg,Yotam Drier

Respiratory research 25:176 PubMed38658970

2024

Downregulation of HMGCS2 mediated AECIIs lipid metabolic alteration promotes pulmonary fibrosis by activating fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Juntang Yang,Xin Pan,Min Xu,Yingge Li,Chenxi Liang,Lulu Liu,Zhongzheng Li,Lan Wang,Guoying Yu

Immunity, inflammation and disease 12:e1191 PubMed38477658

2024

Hmgcs2 is the hub gene in diabetic cardiomyopathy and is negatively regulated by Hmgcs2, promoting high glucose-induced cardiomyocyte injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang,Li-Feng Ping,Fu-Yan Bai,Xin-Huan Zhang,Guang-Hong Li

British journal of cancer 130:1249-1260 PubMed38361045

2024

AHRR and SFRP2 in primary versus recurrent high-grade serous ovarian carcinoma and their prognostic implication.

Applications

Unspecified application

Species

Unspecified reactive species

Nanna Monjé,Mihnea P Dragomir,Bruno V Sinn,Inga Hoffmann,Anuar Makhmut,Tincy Simon,Catarina A Kunze,Jana Ihlow,Wolfgang D Schmitt,Jonathan Pohl,Iris Piwonski,Sofya Marchenko,Carlotta Keunecke,Teodor G Calina,Francesca Tiso,Hagen Kulbe,Caroline Kreuzinger,Dan Cacsire Castillo-Tong,Jalid Sehouli,Elena I Braicu,Carsten Denkert,Silvia Darb-Esfahani,Kirsten Kübler,David Capper,Fabian Coscia,Markus Morkel,David Horst,Christine Sers,Eliane T Taube

The Journal of biological chemistry 300:105617 PubMed38176653

2024

Ketone bodies promote epididymal white adipose expansion to alleviate liver steatosis in response to a ketogenic diet.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Fei Zhao,Xin-Ge Zhang,Yi-Ping Tang,Ying-Xi Zhu,Hong-Yu Nie,Dan-Dan Bu,Lei Fang,Chao-Jun Li

Journal of lipid research 64:100440 PubMed37826876

2023

Metabolic switch from glycogen to lipid in the liver maintains glucose homeostasis in neonatal mice.

Applications

Unspecified application

Species

Unspecified reactive species

Liangkui Li,Haoyu Zhou,Jinhui Wang,Jiaxin Li,Xuchao Lyu,Wenshan Wang,Chengting Luo,He Huang,Dawang Zhou,Xiaowei Chen,Li Xu,Peng Li

Aging 15:7794-7810 PubMed37561521

2023

Hmgcs2 regulates M2 polarization of macrophages to repair myocardial injury induced by sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Zheng Zou,Jun-Feng Hao,Ming-Xiao Hou

Journal of translational medicine 21:66 PubMed36726122

2023

Role of mitochondrial metabolic disorder and immune infiltration in diabetic cardiomyopathy: new insights from bioinformatics analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Peng,Yanxiu Zhang,Xueyan Lang,Yao Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HMGCS2 antibody [EPR8642]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (AB137043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (AB137043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (AB137043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (AB137043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (AB137043)

Western blot - Anti-HMGCS2 antibody [EPR8642] (AB137043)

Western blot - Anti-HMGCS2 antibody [EPR8642] (AB137043)

Western blot - Anti-HMGCS2 antibody [EPR8642] (AB137043)

Western blot - Anti-HMGCS2 antibody [EPR8642] (AB137043)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (46)

Product promise