Rabbit Recombinant Monoclonal HMGN1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra), IP and reacts with Human, Transfected cell line - Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (AB314865), expandable thumbnail](https://content.abcam.com/products/images/hmgn1-antibody-epr26772-17-bsa-and-azide-free-ab314865--flow-cytometry-intracellular-img423444.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (AB314865)")
![Immunocytochemistry/ Immunofluorescence - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (AB314865), expandable thumbnail](https://content.abcam.com/products/images/hmgn1-antibody-epr26772-17-bsa-and-azide-free-ab314865--immunocytochemistry-immunofluorescence-img423431.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (AB314865)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (AB314865), expandable thumbnail](https://content.abcam.com/products/images/hmgn1-antibody-epr26772-17-bsa-and-azide-free-ab314865--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423400.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (AB314865)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (AB314865), expandable thumbnail](https://content.abcam.com/products/images/hmgn1-antibody-epr26772-17-bsa-and-azide-free-ab314865--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423399.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (AB314865)")
![Immunoprecipitation - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (AB314865), expandable thumbnail](https://content.abcam.com/products/images/hmgn1-antibody-epr26772-17-bsa-and-azide-free-ab314865--immunoprecipitation-img423377.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (AB314865)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | Flow Cyt (Intra) | IP | WB | ChIP-seq | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell line - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Transfected cell line - Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
ChIP-seq
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 (By similarity).
Alternative names
HMG14, HMGN1, Non-histone chromosomal protein HMG-14, High mobility group nucleosome-binding domain-containing protein 1
Recommended products
Rabbit Recombinant Monoclonal HMGN1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra), IP and reacts with Human, Transfected cell line - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26772-17
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody does not cross-react with human HMGN2, HMGN3, HMGN4, HMGN5.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab314865 is the carrier-free version of Anti-HMGN1 antibody [EPR26772-17] ab314864.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HMGN1 also known as high mobility group nucleosomal binding domain protein 1 is a non-histone chromosomal protein with a mass of approximately 11 kDa. It interacts with chromatin inside the nucleus and regulates the architecture of nucleosomes. HMGN1 expresses in various types of tissues with higher levels observed in actively proliferating cells. The protein modulates the structure of chromatin by binding to nucleosomes and affecting nucleosome spacing and histone modifications.
- Biological function summary
This protein plays an important role in modulating gene expression and cellular response to DNA damage. It does not form part of a large complex but associates with nucleosomes to influence chromatin dynamics. HMGN1 can affect transcription by altering chromatin accessibility and stabilizer that facilitates repair processes in response to DNA damage. The protein can also interfere with specific transcription factors therefore reducing or enhancing the transcription of target genes.
- Pathways
This protein fits into cellular pathways involving transcription regulation and DNA repair. HMGN1 modifies chromatin structure and therefore participates in transcription-related pathways. It is also involved in DNA repair pathways ensuring proper response to DNA damage. Within these systems HMGN1 interacts with proteins such as HMGA1 influencing transcription and ATM which is critical in the DNA damage response pathway.
- Associated diseases and disorders
Irregular expression or dysfunction of this protein links to certain cancers and autoimmune disorders. HMGN1 influences the development and progression of diseases through its role in gene expression and chromatin modulation. Aberrations in HMGN1 expression can lead to improper regulation of related proteins such as p53 an important player in cancer progression and NF-kB involved in immune responses. This connection with p53 and NF-kB makes HMGN1 a target of interest in disease research and therapy development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Flow Cytometry (Intracellular) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865), expandable thumbnail](https://content.abcam.com/products/images/hmgn1-antibody-epr26772-17-bsa-and-azide-free-ab314865--flow-cytometry-intracellular-img423444.jpg "Flow Cytometry (Intracellular) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865)")
Flow Cytometry (Intracellular) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865)
This data was developed using Anti-HMGN1 antibody [EPR26772-17] ab314864, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NCCIT (human pluripotent embryonic carcinoma epithelial cell) cells labelling HMGN1 with Anti-HMGN1 antibody [EPR26772-17] ab314864 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865), expandable thumbnail](https://content.abcam.com/products/images/hmgn1-antibody-epr26772-17-bsa-and-azide-free-ab314865--immunocytochemistry-immunofluorescence-img423431.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865)")
Immunocytochemistry/ Immunofluorescence - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865)
This data was developed using Anti-HMGN1 antibody [EPR26772-17] ab314864, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NCCIT (human pluripotent embryonic carcinoma epithelial cell) cells labelling HMGN1 with Anti-HMGN1 antibody [EPR26772-17] ab314864 at 1/50 (10.34 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing nuclear staining in NCCIT cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865), expandable thumbnail](https://content.abcam.com/products/images/hmgn1-antibody-epr26772-17-bsa-and-azide-free-ab314865--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423400.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865)
This data was developed using Anti-HMGN1 antibody [EPR26772-17] ab314864, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a HMGN1 expression vector containing a Myc-His tag. (B) HEK-293T transfected with empty vector containing a Myc-His tag. tissue labeling HMGN1 with Anti-HMGN1 antibody [EPR26772-17] ab314864 at 1/2000 (0.266 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on HEK-293T transfected with a Myc-His-tagged HMGN1 construct (image A). No staining on HEK-293T transfected with empty plasmid (image B). The section was incubated with Anti-HMGN1 antibody [EPR26772-17] ab314864 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865), expandable thumbnail](https://content.abcam.com/products/images/hmgn1-antibody-epr26772-17-bsa-and-azide-free-ab314865--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423399.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865)
This data was developed using Anti-HMGN1 antibody [EPR26772-17] ab314864, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling HMGN1 with Anti-HMGN1 antibody [EPR26772-17] ab314864 at 1/200 (2.655 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human breast carcinoma. The section was incubated with Anti-HMGN1 antibody [EPR26772-17] ab314864 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunoprecipitation - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865), expandable thumbnail](https://content.abcam.com/products/images/hmgn1-antibody-epr26772-17-bsa-and-azide-free-ab314865--immunoprecipitation-img423377.jpg "Immunoprecipitation - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865)")
Immunoprecipitation - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865)
This data was developed using Anti-HMGN1 antibody [EPR26772-17] ab314864, the same antibody clone in a different buffer formulation.
HMGN1 was immunoprecipitated from 0.35 mg NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate with Anti-HMGN1 antibody [EPR26772-17] ab314864 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-HMGN1 antibody [EPR26772-17] ab314864 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
Lane 2: Anti-HMGN1 antibody [EPR26772-17] ab314864 IP in NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-HMGN1 antibody [EPR26772-17] ab314864 in NCCIT whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-HMGN1 antibody [EPR26772-17] (Anti-HMGN1 antibody [EPR26772-17] ab314864) at 1/30 dilution
All lanes: NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865), expandable thumbnail](https://content.abcam.com/products/images/hmgn1-antibody-epr26772-17-bsa-and-azide-free-ab314865--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423398.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865)
This data was developed using Anti-HMGN1 antibody [EPR26772-17] ab314864, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast tissue labeling HMGN1 with Anti-HMGN1 antibody [EPR26772-17] ab314864 at 1/200 (2.655 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human breast. The section was incubated with Anti-HMGN1 antibody [EPR26772-17] ab314864 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865), expandable thumbnail](https://content.abcam.com/products/images/hmgn1-antibody-epr26772-17-bsa-and-azide-free-ab314865--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423398.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN1 antibody [EPR26772-17] - BSA and Azide free (ab314865)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com