Rabbit Recombinant Monoclonal HMGN1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-HMGN1 antibody [EPR27314-37] - BSA and Azide free (Capture) (AB315930), expandable thumbnail](https://content.abcam.com/products/images/hmgn1-antibody-epr27314-37-bsa-and-azide-free-capture-ab315930--sandwich-elisa-img426058.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-HMGN1 antibody [EPR27314-37] - BSA and Azide free (Capture) (AB315930)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 (By similarity).
Alternative names
Non-histone chromosomal protein HMG-14, High mobility group nucleosome-binding domain-containing protein 1, HMG14, HMGN1
Recommended products
Rabbit Recombinant Monoclonal HMGN1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Alternative names
Non-histone chromosomal protein HMG-14, High mobility group nucleosome-binding domain-containing protein 1, HMG14, HMGN1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR27314-37
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab315930 is a BSA and Azide free antibody supplied in an unconjugated
format and it is suitable for sandwich ELISAs to quantify Human HMGN1.
The recommended pair for sandwich ELISA is:
Capture: ab315930, Human HMGN1 Capture Antibody (unconjugated)
Detector: Anti-HMGN1 antibody [EPR26772-11] - BSA and Azide free (Detector) ab315931, Human HMGN1 Detector Antibody (unconjugated)
The reference range value is 0.16-10 ng/mL.
The recommended antibody orientation is based on internal optimization
for ELISAbased assays. Antibody orientation is assay dependent and
needs to be optimized for each assay type. Please note that the
range provided for this antibody is only an estimation based on the
performance of the product using the recommended antibody pair.
Performance of the antibody pair will depend on the specific
characteristics of your assay. We guarantee the product works in
sandwich ELISA, but we do not guarantee the sensitivity or dynamic
range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA,
sodium azide and glycerol for higher conjugation efficiency.
The antibodies are provided at an approximate concentration of
1 mg/ml as measured by the protein A280 method. Use our conjugation
kits for antibody conjugates that are ready-to-use in as
little as 20 minutes with <1 minute hands-on-time and 100% antibody
recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HMGN1 also known as high mobility group nucleosomal binding domain protein 1 is a non-histone chromosomal protein with a mass of approximately 11 kDa. It interacts with chromatin inside the nucleus and regulates the architecture of nucleosomes. HMGN1 expresses in various types of tissues with higher levels observed in actively proliferating cells. The protein modulates the structure of chromatin by binding to nucleosomes and affecting nucleosome spacing and histone modifications.
- Biological function summary
This protein plays an important role in modulating gene expression and cellular response to DNA damage. It does not form part of a large complex but associates with nucleosomes to influence chromatin dynamics. HMGN1 can affect transcription by altering chromatin accessibility and stabilizer that facilitates repair processes in response to DNA damage. The protein can also interfere with specific transcription factors therefore reducing or enhancing the transcription of target genes.
- Pathways
This protein fits into cellular pathways involving transcription regulation and DNA repair. HMGN1 modifies chromatin structure and therefore participates in transcription-related pathways. It is also involved in DNA repair pathways ensuring proper response to DNA damage. Within these systems HMGN1 interacts with proteins such as HMGA1 influencing transcription and ATM which is critical in the DNA damage response pathway.
- Associated diseases and disorders
Irregular expression or dysfunction of this protein links to certain cancers and autoimmune disorders. HMGN1 influences the development and progression of diseases through its role in gene expression and chromatin modulation. Aberrations in HMGN1 expression can lead to improper regulation of related proteins such as p53 an important player in cancer progression and NF-kB involved in immune responses. This connection with p53 and NF-kB makes HMGN1 a target of interest in disease research and therapy development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-HMGN1 antibody [EPR27314-37] - BSA and Azide free (Capture) (ab315930), expandable thumbnail](https://content.abcam.com/products/images/hmgn1-antibody-epr27314-37-bsa-and-azide-free-capture-ab315930--sandwich-elisa-img426058.jpg "Sandwich ELISA - Anti-HMGN1 antibody [EPR27314-37] - BSA and Azide free (Capture) (ab315930)")
Sandwich ELISA - Anti-HMGN1 antibody [EPR27314-37] - BSA and Azide free (Capture) (ab315930)
Sandwich ELISA of Human HMGN1 Antibody Pair - BSA and Azide free ab315929 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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