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Rabbit Recombinant Monoclonal HMGN2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-HMGN2 antibody [EPR7091] (AB124997), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN2 antibody [EPR7091] (AB124997), expandable thumbnail
  • Western blot - Anti-HMGN2 antibody [EPR7091] (AB124997), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWB
Human
Tested
Not recommended
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1/250 - 1/500

Notes

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal HMGN2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR7091

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

HMGN2 also known as high mobility group nucleosome binding domain 2 is a small protein weighing approximately 10.1 kDa. It is a non-histone chromosomal protein that interacts directly with nucleosomes modulating chromatin structure and function. HMGN2 is widely expressed in various tissues and cells and it plays a role in gene regulation by affecting the chromatin compaction and transcriptional activity.

Biological function summary

HMGN2 influences cellular processes by binding to the nucleosome core particle within chromatin. It is a component of a dynamic chromatin complex that affects gene expression by remodeling chromatin structure making specific genomic regions more accessible to transcription machinery. HMGN2's interaction with nucleosomes can regulate a host of genes involved in growth and differentiation highlighting its role in modulating cellular functions.

Pathways

Chromatin remodeling and transcriptional regulation involve HMGN2 as a significant player. It participates in important pathways like gene expression regulation and cellular responses to DNA damage. HMGN2 interacts with proteins such as histones and other high mobility group proteins which orchestrate these pathways by modifying chromatin architecture and influencing transcription factor access.

Associated diseases and disorders

HMGN2 shows relations to cancer and autoimmune diseases. Altered expression or mutations in HMGN2 can disrupt normal chromatin dynamics contributing to oncogenesis by deregulating genes involved in cell cycle control. Additionally autoimmune conditions may arise due to the immune system's aberrant response to misregulated HMGN2 affecting proteins such as histones that interact with this target. Understanding HMGN2's role in disease can offer insight into potential therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-HMGN2 antibody [EPR7091] (ab124997), expandable thumbnail

    Western blot - Anti-HMGN2 antibody [EPR7091] (ab124997)

    Blocking/Diluting Buffer and concentration:
    5% NFDM/TBST

    All lanes: Western blot - Anti-HMGN2 antibody [EPR7091] (ab124997) at 1/1000 dilution

    Lane 1: HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate prepared in 1%SDS Hot lysis method at 15 µg

    Lane 2: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate prepared in 1%SDS Hot lysis method at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 9 kDa

    Observed band size: 18 kDa

    This data was developed using ab124997, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN2 antibody [EPR7091] (ab124997), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN2 antibody [EPR7091] (ab124997)

    ab124997 at 1/250 dilution staining HMGN2 in paraffin-embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-HMGN2 antibody [EPR7091] (ab124997), expandable thumbnail

    Western blot - Anti-HMGN2 antibody [EPR7091] (ab124997)

    All cell lysates were prepared in 1%SDS Hot lysis method.

    All lanes: Western blot - Anti-HMGN2 antibody [EPR7091] (ab124997) at 1/1000 dilution

    Lane 1: HL60 cell lysate at 10 µg

    Lane 2: K562 cell lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Lane 4: SH-SY5Y cell lysate at 10 µg

    Lane 5: Jurkat cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 9 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com