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AB248090

Anti-HMGN2 antibody [EPR7091] - BSA and Azide free

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Rabbit Recombinant Monoclonal HMGN2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

HMG17, HMGN2, Non-histone chromosomal protein HMG-17, High mobility group nucleosome-binding domain-containing protein 2

3 Images
Western blot - Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (AB248090)
  • WB

Unknown

Western blot - Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (AB248090)

This data was developed using ab124997, the same antibody clone in a different buffer formulation.

All cell lysates were prepared in 1% SDS Hot lysis method.

All lanes:

Western blot - Anti-HMGN2 antibody [EPR7091] (<a href='/en-us/products/primary-antibodies/hmgn2-antibody-epr7091-ab124997'>ab124997</a>) at 1/1000 dilution

Lane 1:

HL60 cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

SH-SY5Y cell lysate at 10 µg

Lane 5:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 9 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (AB248090)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (AB248090)

This data was developed using ab124997, the same antibody clone in a different buffer formulation.

ab124997 at 1/250 dilution staining HMGN2 in paraffin-embedded Human colon tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (AB248090)
  • WB

Lab

Western blot - Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (AB248090)

This data was developed using ab124997, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HMGN2 antibody [EPR7091] (<a href='/en-us/products/primary-antibodies/hmgn2-antibody-epr7091-ab124997'>ab124997</a>) at 1/1000 dilution

Lane 1:

HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate prepared in 1%SDS Hot lysis method at 15 µg

Lane 2:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate prepared in 1%SDS Hot lysis method at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 9 kDa

Observed band size: 18 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7091

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HMGN2 also known as high mobility group nucleosome binding domain 2 is a small protein weighing approximately 10.1 kDa. It is a non-histone chromosomal protein that interacts directly with nucleosomes modulating chromatin structure and function. HMGN2 is widely expressed in various tissues and cells and it plays a role in gene regulation by affecting the chromatin compaction and transcriptional activity.
Biological function summary

HMGN2 influences cellular processes by binding to the nucleosome core particle within chromatin. It is a component of a dynamic chromatin complex that affects gene expression by remodeling chromatin structure making specific genomic regions more accessible to transcription machinery. HMGN2's interaction with nucleosomes can regulate a host of genes involved in growth and differentiation highlighting its role in modulating cellular functions.

Pathways

Chromatin remodeling and transcriptional regulation involve HMGN2 as a significant player. It participates in important pathways like gene expression regulation and cellular responses to DNA damage. HMGN2 interacts with proteins such as histones and other high mobility group proteins which orchestrate these pathways by modifying chromatin architecture and influencing transcription factor access.

HMGN2 shows relations to cancer and autoimmune diseases. Altered expression or mutations in HMGN2 can disrupt normal chromatin dynamics contributing to oncogenesis by deregulating genes involved in cell cycle control. Additionally autoimmune conditions may arise due to the immune system's aberrant response to misregulated HMGN2 affecting proteins such as histones that interact with this target. Understanding HMGN2's role in disease can offer insight into potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation (By similarity).
See full target information HMGN2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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