AB186001

Anti-HMGN5 antibody

Name: Anti-HMGN5 antibody (ab186001) | Abcam
Brand: Abcam Limited
SKU: AB186001
Price: 520 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal HMGN5 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HMGN5 aa 200 to C-terminus.

View Alternative Names

NSBP1, HMGN5, High mobility group nucleosome-binding domain-containing protein 5, Nucleosome-binding protein 1

2 Images

Supplier Data

Immunoprecipitation - Anti-HMGN5 antibody (AB186001)

Detection of Human HMGN5 by Western Blot of Immunoprecipitates.
Lane 1 : ab186001 used for immunoprecipitation (IP) at 6 μg/mg of lysate from HeLa cells (1 mg for IP; 20% of IP loaded)
Lane 2 : Control IgG.
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-HMGN5 antibody (ab186001)

Predicted band size: 32 kDa

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Supplier Data

Western blot - Anti-HMGN5 antibody (AB186001)

All lanes:

Western blot - Anti-HMGN5 antibody (ab186001) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 32 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human HMGN5 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P82970

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HMGN5 protein also known as Nsbp1 belongs to the high mobility group (HMG) proteins. It has a molecular mass of approximately 30 kDa. This protein localizes mainly in the nucleus where it binds to nucleosomes influencing chromatin structure and gene expression. Unlike certain other HMG proteins that express in specific tissues HMGN5 shows broad expression across different tissues indicating its varied role in cellular functions.

Biological function summary

HMGN5 functions by modulating chromatin accessibility impacting transcription and replication. It forms part of a complex involved in chromatin remodeling. By affecting chromatin dynamics HMGN5 influences the expression of genes important for cell cycle and differentiation processes. Such influence highlights its role in maintaining genomic stability and regulating cellular responses.

Pathways

HMGN5 plays a role in pathways related to chromatin remodeling and gene transcription. It interacts with proteins involved in the MAPK signaling pathway which is important for cell growth and differentiation. Moreover HMGN5 has associations with the p53 protein pathway contributing to the regulation of cell cycle checkpoints and apoptosis decisions.

HMGN5 has connections to cancer and certain neurological disorders. Its overexpression has been linked to the progression of various cancers such as prostate cancer by altering gene expression and promoting tumorigenesis. In some neurological disorders abnormalities in HMGN5 expression may affect neural cell development and function. Through these conditions HMGN5 often interacts with proteins like p53 and other cell cycle regulators.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction.

See full target information HMGN5

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 17:105933 PubMed40487959

2025

Circular RNA hsa_circRNA_101996 modulates gastric cancer cell proliferation and apoptosis through the miR-577/HMGN5 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Lei Wang,Lin Zhang,Qing Shang

BioMed research international 2019:5630124 PubMed31930127

2019

Hypoxia-Inducible Factor 1A Upregulates HMGN5 by Increasing the Expression of GATA1 and Plays a Role in Osteosarcoma Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Enjie Xu,Zhe Ji,Heng Jiang,Tao Lin,Jun Ma,Xuhui Zhou

Neuron 101:1089-1098.e4 PubMed30713029

2019

Zika Virus Protease Cleavage of Host Protein Septin-2 Mediates Mitotic Defects in Neural Progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Hongda Li,Laura Saucedo-Cuevas,Ling Yuan,Danica Ross,Anide Johansen,Daniel Sands,Valentina Stanley,Alicia Guemez-Gamboa,Anne Gregor,Todd Evans,Shuibing Chen,Lei Tan,Henrik Molina,Nicholas Sheets,Sergey A Shiryaev,Alexey V Terskikh,Amy S Gladfelter,Sujan Shresta,Zhiheng Xu,Joseph G Gleeson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HMGN5 antibody

Immunoprecipitation - Anti-HMGN5 antibody (AB186001)

Western blot - Anti-HMGN5 antibody (AB186001)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise