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AB166609

Anti-HMT antibody [EPR8311]

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(2 Publications)

Rabbit Recombinant Monoclonal HMT antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Histamine N-methyltransferase, HMT, HNMT

1 Images
Western blot - Anti-HMT antibody [EPR8311] (AB166609)
  • WB

Unknown

Western blot - Anti-HMT antibody [EPR8311] (AB166609)

All lanes:

Western blot - Anti-HMT antibody [EPR8311] (ab166609) at 1/1000 dilution

Lane 1:

Fetal liver lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8311

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HMT also known as HMT-H8 or Histone Methyltransferase 8 is an enzyme involved in the transfer of methyl groups to histone proteins an essential step in chromatin modification. It has a molecular mass of approximately 55 kDa. HMT expresses in various tissues with a notable presence in the liver brain and heart tissues. The enzyme's distribution suggests a widespread role in regulating gene expression across different biological systems.
Biological function summary

HMT functions within the context of chromatin remodeling influencing how tightly DNA coiled around histones is therefore impacting gene accessibility. HMT often partners with other proteins to form complexes that precisely regulate methylation status in active transcription sites. By adding methyl groups HMT plays a role in activating or repressing genes affecting a broad range of cellular processes.

Pathways

The enzyme HMT takes part in significant epigenetic pathways such as the histone modification pathway and the gene silencing pathway. It actively interacts with proteins like HDACs (histone deacetylases) within these pathways affecting deacetylation and methylation in chromatin dynamics. HMT's activity often intersects with proteins like DNMTs (DNA methyltransferases) which collaborate to maintain cellular identity through stable gene expression patterns.

Abnormal changes in HMT's activity have been linked to cancer and neurodegenerative diseases. Elevated HMT activity often correlates with certain cancer types where increased methylation leads to the silencing of tumor suppressor genes. In neurodegenerative contexts HMT dysfunction can affect neuronal gene expression contributing to conditions like Huntington's disease. Proteins like p53 and HDAC are often implicated in these disorders alongside HMT as their interactions shift cellular behavior in disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine.
See full target information Histamine N-methyltransferase
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in aging neuroscience 17:1549770 PubMed40365352

2025

Dysregulated calcium signaling in the aged macaque entorhinal cortex associated with tau hyperphosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Shveta Bathla,Dibyadeep Datta,Dinara Bolat,Elizabeth Woo,Alvaro Duque,Jon I Arellano,Amy F T Arnsten,Angus C Nairn

Investigative radiology 52:725-733 PubMed28678084

2017

Magnetic Resonance Lymphography at 9.4 T Using a Gadolinium-Based Nanoparticle in Rats: Investigations in Healthy Animals and in a Hindlimb Lymphedema Model.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Müller,Peter Fries,Bijan Jelvani,François Lux,Claudia E Rübe,Stephanie Kremp,Pietro Giovanoli,Arno Buecker,Michael D Menger,Matthias W Laschke,Florian S Frueh
View all publications
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Product promise

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