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AB249350

Anti-HMT antibody [EPR8311] - BSA and Azide free

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Rabbit Recombinant Monoclonal HMT antibody. Carrier free. Suitable for WB and reacts with Human samples.
1 Images
Western blot - Anti-HMT antibody [EPR8311] - BSA and Azide free (AB249350)
  • WB

Unknown

Western blot - Anti-HMT antibody [EPR8311] - BSA and Azide free (AB249350)

This data was developed using ab166609, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HMT antibody [EPR8311] - BSA and Azide free (ab249350) at 1/1000 dilution

Lane 1:

Fetal liver lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8311

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab249350 is the carrier-free version of ab166609.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HMT also known as HMT-H8 or Histone Methyltransferase 8 is an enzyme involved in the transfer of methyl groups to histone proteins an essential step in chromatin modification. It has a molecular mass of approximately 55 kDa. HMT expresses in various tissues with a notable presence in the liver brain and heart tissues. The enzyme's distribution suggests a widespread role in regulating gene expression across different biological systems.
Biological function summary

HMT functions within the context of chromatin remodeling influencing how tightly DNA coiled around histones is therefore impacting gene accessibility. HMT often partners with other proteins to form complexes that precisely regulate methylation status in active transcription sites. By adding methyl groups HMT plays a role in activating or repressing genes affecting a broad range of cellular processes.

Pathways

The enzyme HMT takes part in significant epigenetic pathways such as the histone modification pathway and the gene silencing pathway. It actively interacts with proteins like HDACs (histone deacetylases) within these pathways affecting deacetylation and methylation in chromatin dynamics. HMT's activity often intersects with proteins like DNMTs (DNA methyltransferases) which collaborate to maintain cellular identity through stable gene expression patterns.

Abnormal changes in HMT's activity have been linked to cancer and neurodegenerative diseases. Elevated HMT activity often correlates with certain cancer types where increased methylation leads to the silencing of tumor suppressor genes. In neurodegenerative contexts HMT dysfunction can affect neuronal gene expression contributing to conditions like Huntington's disease. Proteins like p53 and HDAC are often implicated in these disorders alongside HMT as their interactions shift cellular behavior in disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine.
See full target information Histamine N-methyltransferase

Alternative Names

Histamine N-methyltransferase, HMT, HNMT
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com