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AB79653

Anti-HMW Kininogen antibody

3

(1 Review)

|

(3 Publications)

Rabbit Polyclonal Kininogen antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human KNG1.

View Alternative Names

BDK, KNG, KNG1, Kininogen-1, Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, High molecular weight kininogen, Williams-Fitzgerald-Flaujeac factor, HMWK

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-HMW Kininogen antibody (AB79653)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HMW Kininogen antibody (AB79653)

ICC/IF image of ab79653 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79653, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Native Full Length Protein corresponding to Human KNG1.

P01042

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

High molecular weight kininogen also known as HMW kininogen or HK is a glycoprotein with a molecular mass of about 120 kDa. It is synthesized in the liver and circulates in the bloodstream. It serves as a precursor to bradykinin a peptide involved in inflammation blood pressure regulation and pain. HMW kininogen also acts as a cofactor for kallikrein activation playing a significant role in the intrinsic pathway of blood coagulation. Additionally it exists as part of a quaternary structure with other plasma proteins functioning within the circulatory system.
Biological function summary

This kininogen is essential for the kallikrein-kinin system and interacts with various cell surface receptors to trigger diverse cellular responses. It is part of a complex that includes prekallikrein and factor XII facilitating the assembly and activation of these components on negatively charged surfaces. This interaction supports the generation of bradykinin which mediates vasodilation and increases vascular permeability critical processes in inflammatory responses. The multifunctional nature of HMW kininogen allows it to contribute broadly to the regulation of hemostasis and innate immunity.

Pathways

HMW kininogen is linked notably to both the contact activation pathway and the renin-angiotensin system. In the contact activation pathway it associates closely with prekallikrein and factor XII promoting the conversion of prekallikrein to kallikrein and subsequent steps in coagulation. In the renin-angiotensin system bradykinin produced from HMW kininogen modulates blood pressure through interactions with ACE (angiotensin-converting enzyme) showcasing the enzyme's relationship with these pathways. These pathways position HMW kininogen at a critical junction of cardiovascular and immune system regulation.

HMW kininogen associates with conditions such as hereditary angioedema and cardiovascular disorders. Hereditary angioedema results from bradykinin overproduction implicating HMW kininogen in its pathophysiology with links to defects in C1 inhibitor another regulatory protein. Cardiovascular disorders may involve aberrant bradykinin production and metabolism where HMW kininogen plays a role through interaction with the renin-angiotensin system. Aberrations in its function or expression can contribute toward the pathogenesis of these conditions highlighting the need for further research into its clinical implications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Kininogens are inhibitors of thiol proteases. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes. LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.. Bradykinin. The active peptide bradykinin is a potent vasodilatator that is released from HMW-kininogen shows a variety of physiological effects : (A) influence in smooth muscle contraction, (B) induction of hypotension, (C) natriuresis and diuresis, (D) decrease in blood glucose level, (E) it is a mediator of inflammation and causes (E1) increase in vascular permeability, (E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action).
See full target information KNG1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Parasites & vectors 11:182 PubMed29540224

2018

The blood fluke Schistosoma mansoni cleaves the coagulation protein high molecular weight kininogen (HK) but does not generate the vasodilator bradykinin.

Applications

WB

Species

Unspecified reactive species

Qiang Wang,Akram A Da'dara,Patrick J Skelly

The FEBS journal 283:459-71 PubMed26565144

2015

Plasma biomarkers for neuronal ceroid lipofuscinosis.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha L Hersrud,Ryan D Geraets,Krystal L Weber,Chun-Hung Chan,David A Pearce

Journal of immunology (Baltimore, Md. : 1950) 192:1220-30 PubMed24367026

2013

Generation of multiple fluid-phase C3b:plasma protein complexes during complement activation: possible implications in C3 glomerulopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Mahalakshmi Ramadass,Berhane Ghebrehiwet,Richard J Smith,Richard R Kew
View all publications
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