Anti-HN1 antibody [2C8]

• WB

Unknown

Western blot - Anti-HN1 antibody [2C8] (AB57274)

Western blot against tagged recombinant protein immunogen using ab57274 HN1 antibody at 1ug/ml. Predicted band size of immunogen is 43 kDa

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-HN1 antibody [2C8] (ab57274)

Predicted band size: 16 kDa

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• WB

Lab

Western blot - Anti-HN1 antibody [2C8] (AB57274)

Western blot : Anti-HN1 antibody [2C8] ab57274 staining at 1 µg/mL, shown in green; Rabbit anti GAPDH ab181602 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 25 kDa in Wild-type A549 cell lysates with no signal observed at this size in JPT1 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse HRP (H+L) and Goat anti-Rabbit 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-HN1 antibody [2C8] (ab57274) at 1 µg/mL

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

JPT1 knockout A549 at 20 µg

Lane 3:

HeLa at 20 µg

Lane 4:

A549 Membrane at 20 µg

Secondary

All lanes:

Goat anti-Mouse HRP (H+L) & Goat anti-Rabbit 680RD at 1/20000 dilution

Predicted band size: 16 kDa

Observed band size: 25 kDa,37 kDa

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Exposure time: 10s