AB248138

Anti-HN1 antibody [EPR7364] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal HN1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ARM2, HN1, JPT1, Jupiter microtubule associated homolog 1, Androgen-regulated protein 2, Hematological and neurological expressed 1 protein

2 Images

Flow Cytometry (Intracellular) - Anti-HN1 antibody [EPR7364] - BSA and Azide free (AB248138)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-HN1 antibody [EPR7364] - BSA and Azide free (AB248138)

This data was developed using ab126705, the same antibody clone in a different buffer formulation.

ab126705 at 1/10 dilution staining HN1 in permeabilized HeLa cells by intracellular flow cytometry (red) compared to a rabbit IgG negative control (green).

Unknown

Western blot - Anti-HN1 antibody [EPR7364] - BSA and Azide free (AB248138)

This data was developed using ab126705, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HN1 antibody [EPR7364] (<a href='/en-us/products/primary-antibodies/hn1-antibody-epr7364-ab126705'>ab126705</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Human testis lysate at 10 µg

Lane 4:

Human fetal muscle lysate at 10 µg

Lane 5:

Human thymus lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 16 kDa

false

Unconjugated

Anti-HN1 antibody [EPR7364]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7364

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab248138 is the carrier-free version of ab126705.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HN1 also known as Hematological and Neurological Expressed 1 protein is a small protein with a mass of approximately 10 kDa. It expresses in various tissues mainly in hematopoietic and neurological cells. Researchers also identified its expression in some cancerous tissues which suggests its potential role in tumor biology. HN1 exhibits a notable presence in cytoplasmic and nuclear compartments indicating its involvement in multiple cellular processes.

Biological function summary

Experts believe HN1 participates in cellular development and differentiation. It does not function alone but interacts with other proteins within a larger complex affecting intracellular signaling. This interaction hints at the protein's role in supporting the regulation of cellular growth and hematopoiesis. In addition HN1 influences cellular responses to stress which further supports its relevance in development and disease.

Pathways

HN1 integrates into the Jak-STAT signaling pathway and possibly the Notch signaling pathway. This association with significant signaling pathways implies its role in modulating gene expression linked to cell proliferation and differentiation. HN1 also shows interactions with other proteins in these pathways such as STAT3 suggesting collaborative involvement in regulating cellular responses to external stimuli.

Scientists have linked HN1 to cancer and neurodegenerative diseases. Its upregulation in various cancer types like breast cancer indicates a potential role in tumor cell proliferation and survival. Additionally in neurodegenerative conditions changes in HN1 expression suggest it might contribute to neuronal dysfunction. Connections to related proteins like BCL2 involved in cell survival highlight HN1’s implications in both cancer progression and neurodegenerative pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Modulates negatively AKT-mediated GSK3B signaling (PubMed : 21323578, PubMed : 22155408). Induces CTNNB1 'Ser-33' phosphorylation and degradation through the suppression of the inhibitory 'Ser-9' phosphorylation of GSK3B, which represses the function of the APC : CTNNB1 : GSK3B complex and the interaction with CDH1/E-cadherin in adherent junctions (PubMed : 25169422). Plays a role in the regulation of cell cycle and cell adhesion (PubMed : 25169422, PubMed : 25450365). Has an inhibitory role on AR-signaling pathway through the induction of receptor proteasomal degradation (PubMed : 22155408).

See full target information JPT1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:5945 PubMed36209205

2022

Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxu Ge,Jun Tan,Xianling Dai,Qin Kuang,Shaoyu Zhong,Lili Lai,Chao Yi,Yan Sun,Jing Luo,Chufeng Zhang,Liancai Zhu,Bochu Wang,Minxuan Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HN1 antibody [EPR7364] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-HN1 antibody [EPR7364] - BSA and Azide free (AB248138)

Western blot - Anti-HN1 antibody [EPR7364] - BSA and Azide free (AB248138)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Secondary Antibodies

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Product promise