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AB247030

Anti-HN1L/L11 antibody

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(2 Publications)

Rabbit Polyclonal HN1L/L11 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human JPT2 aa 1-100.

View Alternative Names

C16orf34, HN1L, L11, JPT2, Jupiter microtubule associated homolog 2, Hematological and neurological expressed 1-like protein, HN1-like protein

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-HN1L/L11 antibody (AB247030)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HN1L/L11 antibody (AB247030)

PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling HN1L/L11 using ab247030 at 4 μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HN1L/L11 antibody (AB247030)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HN1L/L11 antibody (AB247030)

Paraffin-embedded human rectum tissue stained for HN1L/L11 using ab247030 at 1/1000 dilution in immunohistochemical analysis.

Western blot - Anti-HN1L/L11 antibody (AB247030)
  • WB

Unknown

Western blot - Anti-HN1L/L11 antibody (AB247030)

All lanes:

Western blot - Anti-HN1L/L11 antibody (ab247030) at 0.4 µg/mL

Lane 1:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (Rat cell line) cell lysate

Predicted band size: 20 kDa

false

Western blot - Anti-HN1L/L11 antibody (AB247030)
  • WB

Supplier Data

Western blot - Anti-HN1L/L11 antibody (AB247030)

All lanes:

Western blot - Anti-HN1L/L11 antibody (ab247030) at 0.4 µg/mL

Lane 1:

RT4 (Human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (Human brain glioma cell line) cell lysate

Predicted band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human JPT2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9H910

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HN1L/L11 protein also known as Hematological and Neurological Expressed 1-Like has a molecular weight of approximately 19 kDa. It is expressed in various tissues including the liver and brain. Mechanically HN1L/L11 is involved in the regulation of cellular growth and proliferation although the exact mechanisms remain under investigation. Researchers often focus on its potential role in modulating gene expression and supporting cellular stress responses.
Biological function summary

HN1L/L11 plays a part in critical cellular functions that support homeostasis. This protein operates within cellular complexes that regulate transcription processes and it may interact with molecular machinery involved in stress adaptation. HN1L/L11's involvement in these essential cellular activities indicates its potential broader impact on maintaining cell survival and integrity in fluctuating environments.

Pathways

HN1L/L11 plays a role in signaling pathways linked to stress response and apoptosis regulation. Notably it is associated with the MAPK and the JAK/STAT pathways which are pivotal in cell survival and differentiation. Through these pathways HN1L/L11 shows interaction with other proteins such as JAK2 and STAT5 all contributing to the orchestration of cellular defense mechanisms under stress conditions.

Researchers have linked HN1L/L11 to various cancer types and neurodegenerative disorders. In cancer the protein's regulation of cell growth pathways implicates its potential role in tumor development and progression often in connection with proteins like p53. In neurodegenerative conditions aberrant expression or function of HN1L/L11 may contribute to neuronal stress and degeneration suggesting it could serve as a biomarker or therapeutic target in such diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nicotinic acid adenine dinucleotide phosphate (NAADP) binding protein required for NAADP-evoked intracellular calcium release (PubMed : 33758061, PubMed : 33758062). Confers NAADP-sensitivity to the two pore channels (TPCs) complex (PubMed : 33758061). Enables NAADP to activate Ca(2+) release from the endoplasmic reticulum through ryanodine receptors (PubMed : 33758062).. (Microbial infection) Involved in the endolysosomal trafficking of human coronavirus SARS-CoV-2.
See full target information JPT2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Science (New York, N.Y.) 374:eabk0410 PubMed34882480

2021

PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation.

Applications

Unspecified application

Species

Unspecified reactive species

Federico Gulluni,Lorenzo Prever,Huayi Li,Petra Krafcikova,Ilaria Corrado,Wen-Ting Lo,Jean Piero Margaria,Anlu Chen,Maria Chiara De Santis,Sophie J Cnudde,Joseph Fogerty,Alex Yuan,Alberto Massarotti,Nasrin Torabi Sarijalo,Oscar Vadas,Roger L Williams,Marcus Thelen,David R Powell,Markus Schueler,Michael S Wiesener,Tamas Balla,Hagit N Baris,Dov Tiosano,Brian M McDermott,Brian D Perkins,Alessandra Ghigo,Miriam Martini,Volker Haucke,Evzen Boura,Giorgio Roberto Merlo,David A Buchner,Emilio Hirsch

Cell biology international 46:83-95 PubMed34519127

2021

HN1L promotes stem cell-like properties by regulating TGF-β signaling pathway through targeting FOXP2 in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shaojun Nong,Zhiwei Wang,Zhongqing Wei,Limin Ma,Yangbo Guan,Jian Ni
View all publications
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Product promise

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