Rabbit Polyclonal HN1L/L11 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human JPT2 aa 1-100.
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Publications
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- Cell biology international 46:83-952021HN1L promotes stem cell-like properties by regulating TGF-β signaling pathway through targeting FOXP2 in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaojun Nong,Zhiwei Wang,Zhongqing Wei,Limin Ma,Yangbo Guan,Jian NiPubMed 34519127
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human JPT2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H910
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Nicotinic acid adenine dinucleotide phosphate (NAADP) binding protein required for NAADP-evoked intracellular calcium release (PubMed:33758061, PubMed:33758062). Confers NAADP-sensitivity to the two pore channels (TPCs) complex (PubMed:33758061). Enables NAADP to activate Ca(2+) release from the endoplasmic reticulum through ryanodine receptors (PubMed:33758062). (Microbial infection) Involved in the endolysosomal trafficking of human coronavirus SARS-CoV-2.
Alternative names
C16orf34, HN1L, L11, JPT2, Jupiter microtubule associated homolog 2, Hematological and neurological expressed 1-like protein, HN1-like protein
Recommended products
Rabbit Polyclonal HN1L/L11 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human JPT2 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human JPT2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H910
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The HN1L/L11 protein also known as Hematological and Neurological Expressed 1-Like has a molecular weight of approximately 19 kDa. It is expressed in various tissues including the liver and brain. Mechanically HN1L/L11 is involved in the regulation of cellular growth and proliferation although the exact mechanisms remain under investigation. Researchers often focus on its potential role in modulating gene expression and supporting cellular stress responses.
- Biological function summary
HN1L/L11 plays a part in critical cellular functions that support homeostasis. This protein operates within cellular complexes that regulate transcription processes and it may interact with molecular machinery involved in stress adaptation. HN1L/L11's involvement in these essential cellular activities indicates its potential broader impact on maintaining cell survival and integrity in fluctuating environments.
- Pathways
HN1L/L11 plays a role in signaling pathways linked to stress response and apoptosis regulation. Notably it is associated with the MAPK and the JAK/STAT pathways which are pivotal in cell survival and differentiation. Through these pathways HN1L/L11 shows interaction with other proteins such as JAK2 and STAT5 all contributing to the orchestration of cellular defense mechanisms under stress conditions.
- Associated diseases and disorders
Researchers have linked HN1L/L11 to various cancer types and neurodegenerative disorders. In cancer the protein's regulation of cell growth pathways implicates its potential role in tumor development and progression often in connection with proteins like p53. In neurodegenerative conditions aberrant expression or function of HN1L/L11 may contribute to neuronal stress and degeneration suggesting it could serve as a biomarker or therapeutic target in such diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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4 product images
Immunocytochemistry/ Immunofluorescence - Anti-HN1L/L11 antibody (ab247030)
PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling HN1L/L11 using ab247030 at 4 μg/ml (green) in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HN1L/L11 antibody (ab247030)
Paraffin-embedded human rectum tissue stained for HN1L/L11 using ab247030 at 1/1000 dilution in immunohistochemical analysis.
Western blot - Anti-HN1L/L11 antibody (ab247030)
All lanes: Western blot - Anti-HN1L/L11 antibody (ab247030) at 0.4 µg/mL
Lane 1: RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 2: U-251 MG (Human brain glioma cell line) cell lysate
Predicted band size: 20 kDa
Western blot - Anti-HN1L/L11 antibody (ab247030)
All lanes: Western blot - Anti-HN1L/L11 antibody (ab247030) at 0.4 µg/mL
Lane 1: NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2: NBT-II (Rat cell line) cell lysate
Predicted band size: 20 kDa
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