AB236759

Anti-HNF-1B antibody [CL0374]

Name: Anti-HNF-1B antibody [CL0374] (ab236759) | Abcam
Brand: Abcam Limited
SKU: AB236759

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(5 Publications)

Mouse Monoclonal HNF-1B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HNF1B aa 1-150.

View Alternative Names

TCF2, HNF1B, Hepatocyte nuclear factor 1-beta, HNF-1-beta, HNF-1B, Homeoprotein LFB3, Transcription factor 2, Variant hepatic nuclear factor 1, TCF-2, vHNF1

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-HNF-1B antibody [CL0374] (AB236759)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HNF-1B antibody [CL0374] (AB236759)

PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling HNF-1B (green) using ab236759 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Paraffin-embedded human tonsil tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis. Negative control.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Paraffin-embedded human renal cancer tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Paraffin-embedded human colorectal cancer tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Paraffin-embedded human colon tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Paraffin-embedded human kidney tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-HNF-1B antibody [CL0374] (AB236759)

All lanes:

Western blot - Anti-HNF-1B antibody [CL0374] (ab236759) at 1 µg/mL

Lane 1:

Caco-2 (human colorectal adenocarcinoma cell line) cell lysate

Lane 2:

A431 (human epidermoid carcinoma cell line) cell lysate

Predicted band size: 61 kDa

false

Supplier Data

Western blot - Anti-HNF-1B antibody [CL0374] (AB236759)

All lanes:

Western blot - Anti-HNF-1B antibody [CL0374] (ab236759) at 1 µg/mL

Lane 1:

Negative control, vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

HNF-1B over-expression (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK-293T cells) cell lysate

Predicted band size: 61 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0374

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human HNF1B aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P35680

Epitope

Binds to an epitope located within the peptide sequence TPPILKELQA as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HNF-1B also known as hepatocyte nuclear factor 1-beta is a transcription factor with a molecular mass of approximately 56 kDa. This protein regulates gene expression by binding to specific DNA sequences influencing the transcriptional activity of target genes. HNF-1B is highly expressed in tissues such as the kidney liver pancreas and lung. It plays a critical role in the development and function of these organs exerting control over the expression of genes involved in their respective physiological processes.

Biological function summary

HNF-1B functions are integral to organ development and tissue-specific gene expression. It is part of the HNF family of transcription factors and forms complexes with other members like HNF-1A. HNF-1B regulates genes that are essential for epithelial cell differentiation and organ morphogenesis. This regulation ensures proper functioning of multiple organs highlighting its significance in maintaining organ health and homeostasis.

Pathways

HNF-1B is deeply integrated into the Wnt signaling pathway and renal-specific pathways. It modulates the expression of key components within these pathways such as promoting renal tubule development and function. HNF-1B interacts with other proteins like TCF7L2 and β-catenin facilitating its role in Wnt signaling. These interactions guide precise cellular responses allowing HNF-1B to coordinate complex biological processes like organogenesis and cellular differentiation.

Mutations in the gene encoding HNF-1B contribute to renal cysts and diabetes syndrome (RCAD) and maturity-onset diabetes of the young type 5 (MODY5). These genetic variations disrupt HNF-1B function leading to impaired organ development and function. In RCAD HNF-1B abnormalities link closely to kidney malformations and diabetic symptoms interacting with genes like PAX2 involved in kidney formation highlighting the molecular complexity associated with these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed : 17924661, PubMed : 7900999). Binds to the FPC element in the cAMP regulatory unit of the PLAU gene (By similarity). Transcriptional activity is increased by coactivator PCBD1 (PubMed : 24204001).

See full target information HNF1B

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Lupus science & medicine 11: PubMed39608815

2024

HNF-1β alleviates podocyte injury in lupus nephritis by maintaining endoplasmic reticulum homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Mei Zou,Jie Yu,Yuan-Yuan Ruan,Ying Xie,Xiao-Min An,Pei-Lei Chen,Ying-Qin Luo,Ming-Jun Shi,Miao Liu,Li-Fen Xu,Jun Liu,Bing Guo,Fan Zhang

JCI insight 7: PubMed34874920

2022

Angiotensin-(1-7)/MasR axis promotes migration of monocytes/macrophages with a regulatory phenotype to perform phagocytosis and efferocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Isabella Zaidan,Luciana P Tavares,Michelle A Sugimoto,Kátia M Lima,Graziele L Negreiros-Lima,Lívia Cr Teixeira,Thais C Miranda,Bruno Vs Valiate,Allysson Cramer,Juliana Priscila Vago,Gabriel H Campolina-Silva,Jéssica Am Souza,Laís C Grossi,Vanessa Pinho,Maria Jose Campagnole-Santos,Robson As Santos,Mauro M Teixeira,Izabela Galvão,Lirlândia P Sousa

Oncology letters 23:9 PubMed34820008

2021

Role of Lamin A and emerin in maintaining nuclear morphology in different subtypes of ovarian epithelial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shiori Watabe,Sayaka Kobayashi,Mizuho Hatori,Yoshimi Nishijima,Naoki Inoue,Hayato Ikota,Akira Iwase,Hideaki Yokoo,Masanao Saio

Cell stem cell 28:1105-1124.e19 PubMed33915078

2021

Modeling plasticity and dysplasia of pancreatic ductal organoids derived from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Markus Breunig,Jessica Merkle,Martin Wagner,Michael K Melzer,Thomas F E Barth,Thomas Engleitner,Johannes Krumm,Sandra Wiedenmann,Christian M Cohrs,Lukas Perkhofer,Gaurav Jain,Jana Krüger,Patrick C Hermann,Maximilian Schmid,Tamara Madácsy,Árpád Varga,Joscha Griger,Ninel Azoitei,Martin Müller,Oliver Wessely,Pamela G Robey,Sandra Heller,Zahra Dantes,Maximilian Reichert,Cagatay Günes,Christian Bolenz,Florian Kuhn,József Maléth,Stephan Speier,Stefan Liebau,Bence Sipos,Bernhard Kuster,Thomas Seufferlein,Roland Rad,Matthias Meier,Meike Hohwieler,Alexander Kleger

Oncology letters 20:80 PubMed32863913

2020

miR-375-3p inhibits the progression of laryngeal squamous cell carcinoma by targeting hepatocyte nuclear factor-1β.

Applications

Unspecified application

Species

Unspecified reactive species

Kunpeng Chang,Zhenxing Wei,Hua Cao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-HNF-1B antibody [CL0374]

Immunocytochemistry/ Immunofluorescence - Anti-HNF-1B antibody [CL0374] (AB236759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Western blot - Anti-HNF-1B antibody [CL0374] (AB236759)

Western blot - Anti-HNF-1B antibody [CL0374] (AB236759)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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