Rabbit Recombinant Monoclonal HNF-1B antibody. Carrier free. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (AB238980), expandable thumbnail](https://content.abcam.com/products/images/hnf-1b-antibody-epr18644-13-bsa-and-azide-free-ab238980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115019.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (AB238980)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (AB238980), expandable thumbnail](https://content.abcam.com/products/images/hnf-1b-antibody-epr18644-13-bsa-and-azide-free-ab238980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56825.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (AB238980)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (AB238980), expandable thumbnail](https://content.abcam.com/products/images/hnf-1b-antibody-epr18644-13-bsa-and-azide-free-ab238980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113797.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (AB238980)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (AB238980), expandable thumbnail](https://content.abcam.com/products/images/hnf-1b-antibody-epr18644-13-bsa-and-azide-free-ab238980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81002.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (AB238980)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (AB238980), expandable thumbnail](https://content.abcam.com/products/images/hnf-1b-antibody-epr18644-13-bsa-and-azide-free-ab238980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129896.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (AB238980)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
3 products for Alternative Product
1 product for Alternative Version
Target data
Function
Transcription factor that binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed:17924661, PubMed:7900999). Binds to the FPC element in the cAMP regulatory unit of the PLAU gene (By similarity). Transcriptional activity is increased by coactivator PCBD1 (PubMed:24204001).
Alternative names
TCF2, HNF1B, Hepatocyte nuclear factor 1-beta, HNF-1-beta, HNF-1B, Homeoprotein LFB3, Transcription factor 2, Variant hepatic nuclear factor 1, TCF-2, vHNF1
Recommended products
Rabbit Recombinant Monoclonal HNF-1B antibody. Carrier free. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18644-13
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab238980 is the carrier-free version of Anti-HNF-1B antibody [EPR18644-13] ab213149.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HNF-1B also known as hepatocyte nuclear factor 1-beta is a transcription factor with a molecular mass of approximately 56 kDa. This protein regulates gene expression by binding to specific DNA sequences influencing the transcriptional activity of target genes. HNF-1B is highly expressed in tissues such as the kidney liver pancreas and lung. It plays a critical role in the development and function of these organs exerting control over the expression of genes involved in their respective physiological processes.
- Biological function summary
HNF-1B functions are integral to organ development and tissue-specific gene expression. It is part of the HNF family of transcription factors and forms complexes with other members like HNF-1A. HNF-1B regulates genes that are essential for epithelial cell differentiation and organ morphogenesis. This regulation ensures proper functioning of multiple organs highlighting its significance in maintaining organ health and homeostasis.
- Pathways
HNF-1B is deeply integrated into the Wnt signaling pathway and renal-specific pathways. It modulates the expression of key components within these pathways such as promoting renal tubule development and function. HNF-1B interacts with other proteins like TCF7L2 and β-catenin facilitating its role in Wnt signaling. These interactions guide precise cellular responses allowing HNF-1B to coordinate complex biological processes like organogenesis and cellular differentiation.
- Associated diseases and disorders
Mutations in the gene encoding HNF-1B contribute to renal cysts and diabetes syndrome (RCAD) and maturity-onset diabetes of the young type 5 (MODY5). These genetic variations disrupt HNF-1B function leading to impaired organ development and function. In RCAD HNF-1B abnormalities link closely to kidney malformations and diabetic symptoms interacting with genes like PAX2 involved in kidney formation highlighting the molecular complexity associated with these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980), expandable thumbnail](https://content.abcam.com/products/images/hnf-1b-antibody-epr18644-13-bsa-and-azide-free-ab238980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115019.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980)
This data was developed using Anti-HNF-1B antibody [EPR18644-13] ab213149, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling HNF-1B with Anti-HNF-1B antibody [EPR18644-13] ab213149 at 1/4000 dilution, followed by LeicaDS9800 (Bond ™ Polymer Refine Detection). Nuclear staining on mouse liver bile duct is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980), expandable thumbnail](https://content.abcam.com/products/images/hnf-1b-antibody-epr18644-13-bsa-and-azide-free-ab238980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56825.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980)
This data was developed using Anti-HNF-1B antibody [EPR18644-13] ab213149, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling HNF-1B with Anti-HNF-1B antibody [EPR18644-13] ab213149 at 1/4000 dilution, followed by LeicaDS9800 (Bond ™ Polymer Refine Detection). Nuclear staining on rat liver bile duct is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980), expandable thumbnail](https://content.abcam.com/products/images/hnf-1b-antibody-epr18644-13-bsa-and-azide-free-ab238980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113797.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling HNF-1B with Anti-HNF-1B antibody [EPR18644-13] ab213149 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Nuclear staining on human liver bile duct is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HNF-1B antibody [EPR18644-13] ab213149).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980), expandable thumbnail](https://content.abcam.com/products/images/hnf-1b-antibody-epr18644-13-bsa-and-azide-free-ab238980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81002.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980)
Immunohistochemical analysis of paraffin-embedded human liver bile duct carcinoma tissue labeling HNF-1B with Anti-HNF-1B antibody [EPR18644-13] ab213149 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Nuclear staining on human liver bile duct carcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HNF-1B antibody [EPR18644-13] ab213149).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980), expandable thumbnail](https://content.abcam.com/products/images/hnf-1b-antibody-epr18644-13-bsa-and-azide-free-ab238980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129896.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980)
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling HNF-1B with Anti-HNF-1B antibody [EPR18644-13] ab213149 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Negative staining on human hepatocellular carcinoma. Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HNF-1B antibody [EPR18644-13] ab213149).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980), expandable thumbnail](https://content.abcam.com/products/images/hnf-1b-antibody-epr18644-13-bsa-and-azide-free-ab238980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72175.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980)
Immunohistochemical analysis of paraffin-embedded human ovarian clear cell carcinoma tissue labeling HNF-1B with Anti-HNF-1B antibody [EPR18644-13] ab213149 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Nuclear staining on human ovarian clear cell carcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HNF-1B antibody [EPR18644-13] ab213149).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980), expandable thumbnail](https://content.abcam.com/products/images/hnf-1b-antibody-epr18644-13-bsa-and-azide-free-ab238980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107439.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980)
Immunohistochemical analysis of paraffin-embedded human serous ovarian carcinoma tissue labeling HNF-1B with Anti-HNF-1B antibody [EPR18644-13] ab213149 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Negative staining on human serous ovarian carcinoma. Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HNF-1B antibody [EPR18644-13] ab213149).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980), expandable thumbnail](https://content.abcam.com/products/images/hnf-1b-antibody-epr18644-13-bsa-and-azide-free-ab238980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49280.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980)
Immunohistochemical analysis of paraffin-embedded human endometrioid ovary adenocarcinoma tissue labeling HNF-1B with Anti-HNF-1B antibody [EPR18644-13] ab213149 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Negative staining on human endometrioid ovary adenocarcinoma. Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HNF-1B antibody [EPR18644-13] ab213149).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980), expandable thumbnail](https://content.abcam.com/products/images/hnf-1b-antibody-epr18644-13-bsa-and-azide-free-ab238980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72175.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980), expandable thumbnail](https://content.abcam.com/products/images/hnf-1b-antibody-epr18644-13-bsa-and-azide-free-ab238980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107439.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980), expandable thumbnail](https://content.abcam.com/products/images/hnf-1b-antibody-epr18644-13-bsa-and-azide-free-ab238980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49280.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-13] - BSA and Azide free (ab238980)")
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