AB251538

Anti-HNF-1B antibody [EPR18644-37] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal HNF-1B antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Human samples.

View Alternative Names

TCF2, HNF1B, Hepatocyte nuclear factor 1-beta, HNF-1-beta, HNF-1B, Homeoprotein LFB3, Transcription factor 2, Variant hepatic nuclear factor 1, TCF-2, vHNF1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-37] - BSA and Azide free (AB251538)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-37] - BSA and Azide free (AB251538)

This data was developed using ab213150, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human ovarian clear cell carcinoma tissue labeling HNF-1B with ab213150 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Nuclear staining on human ovarian clear cell carcinoma is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-37] - BSA and Azide free (AB251538)

This data was developed using ab213150, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling HNF-1B with ab213150 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Nuclear staining on human liver bile duct is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-37] - BSA and Azide free (AB251538)

This data was developed using ab213150, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human serous ovarian carcinoma tissue labeling HNF-1B with ab213150 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Negative staining on human serous ovarian carcinoma.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-37] - BSA and Azide free (AB251538)

This data was developed using ab213150, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling HNF-1B with ab213150 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Negative staining on human hepatocellular carcinoma.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-37] - BSA and Azide free (AB251538)

This data was developed using ab213150, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver bile duct carcinoma tissue labeling HNF-1B with ab213150 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Nuclear staining on human liver bile duct carcinoma is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-37] - BSA and Azide free (AB251538)

This data was developed using ab213150, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling HNF-1B with ab213150 at 1/2000 dilution followed by LeicaDS9800 (Bond ™ Polymer Refine Detection). Nuclear staining on mouse liver bile duct is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Unconjugated

Anti-HNF-1B antibody [EPR18644-37]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18644-37

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognizes a different epitope of HNF-1B compared to ab213149.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

ab251538 is the carrier-free version of ab213150.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HNF-1B also known as hepatocyte nuclear factor 1-beta is a transcription factor with a molecular mass of approximately 56 kDa. This protein regulates gene expression by binding to specific DNA sequences influencing the transcriptional activity of target genes. HNF-1B is highly expressed in tissues such as the kidney liver pancreas and lung. It plays a critical role in the development and function of these organs exerting control over the expression of genes involved in their respective physiological processes.

Biological function summary

HNF-1B functions are integral to organ development and tissue-specific gene expression. It is part of the HNF family of transcription factors and forms complexes with other members like HNF-1A. HNF-1B regulates genes that are essential for epithelial cell differentiation and organ morphogenesis. This regulation ensures proper functioning of multiple organs highlighting its significance in maintaining organ health and homeostasis.

Pathways

HNF-1B is deeply integrated into the Wnt signaling pathway and renal-specific pathways. It modulates the expression of key components within these pathways such as promoting renal tubule development and function. HNF-1B interacts with other proteins like TCF7L2 and β-catenin facilitating its role in Wnt signaling. These interactions guide precise cellular responses allowing HNF-1B to coordinate complex biological processes like organogenesis and cellular differentiation.

Mutations in the gene encoding HNF-1B contribute to renal cysts and diabetes syndrome (RCAD) and maturity-onset diabetes of the young type 5 (MODY5). These genetic variations disrupt HNF-1B function leading to impaired organ development and function. In RCAD HNF-1B abnormalities link closely to kidney malformations and diabetic symptoms interacting with genes like PAX2 involved in kidney formation highlighting the molecular complexity associated with these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed : 17924661, PubMed : 7900999). Binds to the FPC element in the cAMP regulatory unit of the PLAU gene (By similarity). Transcriptional activity is increased by coactivator PCBD1 (PubMed : 24204001).

See full target information HNF1B

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HNF-1B antibody [EPR18644-37] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-37] - BSA and Azide free (AB251538)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-37] - BSA and Azide free (AB251538)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-37] - BSA and Azide free (AB251538)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-37] - BSA and Azide free (AB251538)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-37] - BSA and Azide free (AB251538)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644-37] - BSA and Azide free (AB251538)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise