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AB209666

Anti-HNF-1B antibody [EPR18644]

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(2 Publications)

Rabbit Recombinant Monoclonal HNF-1B antibody. Suitable for IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

TCF2, HNF1B, Hepatocyte nuclear factor 1-beta, HNF-1-beta, HNF-1B, Homeoprotein LFB3, Transcription factor 2, Variant hepatic nuclear factor 1, TCF-2, vHNF1

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644] (AB209666)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644] (AB209666)

Immunohistochemical analysis of paraffin-embedded Human serous ovarian cancer tissue labeling HNF1 beta with ab209666 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human ovarian serous carcinoma. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644] (AB209666)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644] (AB209666)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HNF1 beta with ab209666 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human colon is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644] (AB209666)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644] (AB209666)

Immunohistochemical analysis of paraffin-embedded Human ovarian clear cell carcinoma tissue labeling HNF1 beta with ab209666 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human ovarian clear cell carcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644] (AB209666)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644] (AB209666)

Immunohistochemical analysis of paraffin-embedded Human ovarian serosity adenocarcinoma tissue labeling HNF1 beta with ab209666 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human ovarian serosity adenocarcinoma. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644] (AB209666)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644] (AB209666)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling HNF1 beta with ab209666 at 1/4000 dilution. Counter stained with Hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644] (AB209666)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644] (AB209666)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling HNF1 beta with ab209666 at 1/4000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Nucleus staining on bile ducts of rat liver is observed. Counter stained with Hematoxylin

Secondary antibody only control : Used PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644] (AB209666)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR18644] (AB209666)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling HNF1 beta with ab209666 at 1/4000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Nucleus staining on bile ducts of mouse liver is observed. Counter stained with Hematoxylin

Secondary antibody only control : Used PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • 421 Alexa Fluor® 405

    Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed

  • 665 Alexa Fluor® 647

    Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18644

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (AB76013)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HNF-1B also known as hepatocyte nuclear factor 1-beta is a transcription factor with a molecular mass of approximately 56 kDa. This protein regulates gene expression by binding to specific DNA sequences influencing the transcriptional activity of target genes. HNF-1B is highly expressed in tissues such as the kidney liver pancreas and lung. It plays a critical role in the development and function of these organs exerting control over the expression of genes involved in their respective physiological processes.
Biological function summary

HNF-1B functions are integral to organ development and tissue-specific gene expression. It is part of the HNF family of transcription factors and forms complexes with other members like HNF-1A. HNF-1B regulates genes that are essential for epithelial cell differentiation and organ morphogenesis. This regulation ensures proper functioning of multiple organs highlighting its significance in maintaining organ health and homeostasis.

Pathways

HNF-1B is deeply integrated into the Wnt signaling pathway and renal-specific pathways. It modulates the expression of key components within these pathways such as promoting renal tubule development and function. HNF-1B interacts with other proteins like TCF7L2 and β-catenin facilitating its role in Wnt signaling. These interactions guide precise cellular responses allowing HNF-1B to coordinate complex biological processes like organogenesis and cellular differentiation.

Mutations in the gene encoding HNF-1B contribute to renal cysts and diabetes syndrome (RCAD) and maturity-onset diabetes of the young type 5 (MODY5). These genetic variations disrupt HNF-1B function leading to impaired organ development and function. In RCAD HNF-1B abnormalities link closely to kidney malformations and diabetic symptoms interacting with genes like PAX2 involved in kidney formation highlighting the molecular complexity associated with these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed : 17924661, PubMed : 7900999). Binds to the FPC element in the cAMP regulatory unit of the PLAU gene (By similarity). Transcriptional activity is increased by coactivator PCBD1 (PubMed : 24204001).
See full target information HNF1B
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:510 PubMed41013592

2025

Culture of pluripotent stem cells in microscale droplets modulates differentiation and tissue patterning towards organoids on chip.

Applications

Unspecified application

Species

Unspecified reactive species

Nadia Vertti-Quintero,Clara Delahousse,Andrey Aristov,Tatiana Traboulsi,Jack-Christophe Cossec,Charles N Baroud,Sébastien Sart

Nature biomedical engineering 9:836-864 PubMed39658630

2024

Heterogeneity-driven phenotypic plasticity and treatment response in branched-organoid models of pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Aristeidis Papargyriou,Mulham Najajreh,David P Cook,Carlo H Maurer,Stefanie Bärthel,Hendrik A Messal,Sakthi K Ravichandran,Till Richter,Moritz Knolle,Thomas Metzler,Akul R Shastri,Rupert Öllinger,Jacob Jasper,Laura Schmidleitner,Surui Wang,Christian Schneeweis,Hellen Ishikawa-Ankerhold,Thomas Engleitner,Laura Mataite,Mariia Semina,Hussein Trabulssi,Sebastian Lange,Aashreya Ravichandra,Maximilian Schuster,Sebastian Mueller,Katja Peschke,Arlett Schäfer,Sophie Dobiasch,Stephanie E Combs,Roland M Schmid,Andreas R Bausch,Rickmer Braren,Irina Heid,Christina H Scheel,Günter Schneider,Anja Zeigerer,Malte D Luecken,Katja Steiger,Georgios Kaissis,Jacco van Rheenen,Fabian J Theis,Dieter Saur,Roland Rad,Maximilian Reichert
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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