AB128912

Anti-HNF-1B antibody [EPR6334(2)]

BOND RX™ Validated
RabMAb
Recombinant
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(7 Publications)

Rabbit Recombinant Monoclonal HNF-1B antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

View Alternative Names

TCF2, HNF1B, Hepatocyte nuclear factor 1-beta, HNF-1-beta, HNF-1B, Homeoprotein LFB3, Transcription factor 2, Variant hepatic nuclear factor 1, TCF-2, vHNF1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR6334(2)] (AB128912)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR6334(2)] (AB128912)

Immunohistochemistry analysis of paraffin-embedded sections of human liver tissue staining HNF-1B wth ab128912 with 1/100 dilution (4.6 μg/mL concentration) and Ready-to-use secondary; counterstained with Hematoxylin. The section was incubated with ab128912 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR6334(2)] (AB128912)

Immunohistochemistry analysis of paraffin-embedded sections of rat liver tissue staining HNF-1B wth ab128912 with 1/2000 dilution (0.23 μg/mL concentration) and Ready-to-use secondary; counterstained with Hematoxylin. The section was incubated with ab128912 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR6334(2)] (AB128912)

Immunohistochemistry analysis of paraffin-embedded sections of mouse liver tissue staining HNF-1B wth ab128912 with 1/2000 dilution (0.23 μg/mL concentration) and Ready-to-use secondary; counterstained with Hematoxylin. The section was incubated with ab128912 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Unknown

Western blot - Anti-HNF-1B antibody [EPR6334(2)] (AB128912)

All lanes:

Western blot - Anti-HNF-1B antibody [EPR6334(2)] (ab128912) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

Caco 2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 61 kDa

false

Carrier free

Anti-HNF-1B antibody [EPR6334(2)] - BSA and Azide free
578 PE

PE Anti-HNF-1B antibody [EPR6334(2)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-HNF-1B antibody [EPR6334(2)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-HNF-1B antibody [EPR6334(2)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-HNF-1B antibody [EPR6334(2)]
660 APC

APC Anti-HNF-1B antibody [EPR6334(2)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-HNF-1B antibody [EPR6334(2)]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-HNF-1B antibody [EPR6334(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6334(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HNF-1B also known as hepatocyte nuclear factor 1-beta is a transcription factor with a molecular mass of approximately 56 kDa. This protein regulates gene expression by binding to specific DNA sequences influencing the transcriptional activity of target genes. HNF-1B is highly expressed in tissues such as the kidney liver pancreas and lung. It plays a critical role in the development and function of these organs exerting control over the expression of genes involved in their respective physiological processes.

Biological function summary

HNF-1B functions are integral to organ development and tissue-specific gene expression. It is part of the HNF family of transcription factors and forms complexes with other members like HNF-1A. HNF-1B regulates genes that are essential for epithelial cell differentiation and organ morphogenesis. This regulation ensures proper functioning of multiple organs highlighting its significance in maintaining organ health and homeostasis.

Pathways

HNF-1B is deeply integrated into the Wnt signaling pathway and renal-specific pathways. It modulates the expression of key components within these pathways such as promoting renal tubule development and function. HNF-1B interacts with other proteins like TCF7L2 and β-catenin facilitating its role in Wnt signaling. These interactions guide precise cellular responses allowing HNF-1B to coordinate complex biological processes like organogenesis and cellular differentiation.

Mutations in the gene encoding HNF-1B contribute to renal cysts and diabetes syndrome (RCAD) and maturity-onset diabetes of the young type 5 (MODY5). These genetic variations disrupt HNF-1B function leading to impaired organ development and function. In RCAD HNF-1B abnormalities link closely to kidney malformations and diabetic symptoms interacting with genes like PAX2 involved in kidney formation highlighting the molecular complexity associated with these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed : 17924661, PubMed : 7900999). Binds to the FPC element in the cAMP regulatory unit of the PLAU gene (By similarity). Transcriptional activity is increased by coactivator PCBD1 (PubMed : 24204001).

See full target information HNF1B

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Hepatology communications 9: PubMed40366121

2025

Single-cell transcription reveals hepatocyte-to-cholangiocyte reprogramming and biliary gene profile in biliary atresia.

Applications

Unspecified application

Species

Unspecified reactive species

Lingdu Meng,Min Du,Haodong Li,Fanyang Kong,Jiajian Yang,Rui Dong,Shan Zheng,Gong Chen,Zhen Shen,Junfeng Wang

Scientific reports 14:22751 PubMed39349582

2024

Protective effects of cilostazol via the HNF1α/FXR signalling pathway and anti-apoptotic mechanisms in a rat model of estrogen-induced intrahepatic cholestasis.

Applications

Unspecified application

Species

Unspecified reactive species

Marwa Hassan,Maha B Salem,Olfat A Hammam,Mohamed ElZallat

Cell regeneration (London, England) 13:19 PubMed39347883

2024

Expression levels and stoichiometry of Hnf1β, Emx2, Pax8 and Hnf4 influence direct reprogramming of induced renal tubular epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xueli Hu,Jianjian Sun,Meng Wan,Bianhong Zhang,Linhui Wang,Tao P Zhong

Physiological genomics 55:565-577 PubMed37720991

2023

Polycystin-2-dependent transcriptome reveals early response of autosomal dominant polycystic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun Jun Jung,Eryn E Dixon,Richard Coleman,Terry Watnick,Jeremy F Reiter,Patricia Outeda,Valeriu Cebotaru,Owen M Woodward,Paul A Welling

Cell cycle (Georgetown, Tex.) 21:1233-1248 PubMed35230916

2022

LncRNA4474 inhibits renal fibrosis by regulating hepatocyte nuclear factor-1β through miR-615 modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Zhu,Zhenyu Wang,Zuohui Liang,Shuangyan Xu,Yirong Teng,Xiaolan Li,Yong Zeng

The Journal of biological chemistry 295:17560-17572 PubMed33453998

2021

Hepatocyte nuclear factor 1β suppresses canonical Wnt signaling through transcriptional repression of lymphoid enhancer-binding factor 1.

Applications

Unspecified application

Species

Unspecified reactive species

Siu Chiu Chan,Sachin S Hajarnis,Sophia M Vrba,Vishal Patel,Peter Igarashi

Scientific reports 8:12960 PubMed30154523

2018

Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Liu,Kevin Van Bortle,Yue Zhang,Ming-Tao Zhao,Joe Z Zhang,Benjamin S Geller,Joshua J Gruber,Chao Jiang,Joseph C Wu,Michael P Snyder

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HNF-1B antibody [EPR6334(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR6334(2)] (AB128912)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR6334(2)] (AB128912)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR6334(2)] (AB128912)

Western blot - Anti-HNF-1B antibody [EPR6334(2)] (AB128912)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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