Anti-HNF-4-alpha antibody [EPR16885-99]
- RabMAb
- Advanced Validation
- Recombinant
- What is this?
4
(2 Reviews)
|
(37 Publications)
Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460) is a rabbit monoclonal antibody detecting HNF-4-alpha in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
View Alternative Names
HNF4, NR2A1, TCF14, HNF4A, Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, Transcription factor HNF-4, TCF-14
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] (AB201460)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HNF-4-alpha with ab201460 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on Human colon tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16885-99] (AB201460)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton-X100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling HNF-4-alpha with ab201460 at 1/2000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution (green).
Confocal image showing nuclear staining on HT-29 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is stained with ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution (red).
-ve control 1 : ab201460 at 1/2000 dilution followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution.
-ve control 2 : ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution.
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-HNF-4-alpha antibody [EPR16885-99] (AB201460)
Intracellular Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) labelling CDKN2A/p16INK4a with purified ab201460 at 1/1000 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] (AB201460)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HNF-4-alpha with ab201460 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on Human liver tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16885-99] (AB201460)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton-X100 permeabilized HT-29 (Human colorectal adenocarcinoma cells) cells labeling HNF-4-alpha with ab201460 at 1/2000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution (green).
Confocal image showing nuclear staining on HT-29 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is stained with ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution (red).
-ve control 1 : ab201460 at 1/2000 dilution followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution.
-ve control 2 : ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] (AB201460)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling HNF-4-alpha with ab201460 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on mouse liver tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] (AB201460)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling HNF-4-alpha with ab201460 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on rat colon tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Supplier Data
Western blot - Anti-HNF-4-alpha antibody [EPR16885-99] (AB201460)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460) at 1/1000 dilution
All lanes:
Caco-2 (Human colorectal adenocarcinoma cells) whole cell lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
false
Exposure time: 15s
- WB
Unknown
Western blot - Anti-HNF-4-alpha antibody [EPR16885-99] (AB201460)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460) at 1/1000 dilution
Lane 1:
Human colon lysate at 10 µg
Lane 2:
Human fetal kidney lysate at 10 µg
Secondary
All lanes:
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
false
Exposure time: 1min
- WB
Supplier Data
Western blot - Anti-HNF-4-alpha antibody [EPR16885-99] (AB201460)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460) at 1/10000 dilution
All lanes:
HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
false
- ChIC/CUT&RUN-seq
Supplier Data
ChIC/CUT&RUN sequencing - Anti-HNF-4-alpha antibody [EPR16885-99] (AB201460)
ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 HepG2 (Human liver hepatocellular carcinoma cell line) cells and 5 µg of ab201460 [EPR16885-99)]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. Additional screenshots of mapped reads can be found in the Protocol booklet in the Product Protocol section. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
- WB
Supplier Data
Western blot - Anti-HNF-4-alpha antibody [EPR16885-99] (AB201460)
Blocking/Dilution buffer : 5% NFDM/TBST.
Based on sequence analysis of the immunogen, ab201460 shares 72% homology with HNF4 gamma. The levels of XR were tested in the accompanying recombinant protein, WB experiment.
All lanes:
Western blot - Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460) at 1/1000 dilution
All lanes:
Full length mouse HNF-4-gamma recombinant protein at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa
false
Exposure time: 3min
Related conjugates and formulations (5)
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Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free
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565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-HNF-4-alpha antibody [EPR16885-99]
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617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-HNF-4-alpha antibody [EPR16885-99]
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-HNF-4-alpha antibody [EPR16885-99]
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-HNF-4-alpha antibody [EPR16885-99]
Reactivity data
Product details
What is this antibody validated in?
Anti-HNF-4-alpha antibody [EPR16885-99] (ab201460) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of HNF-4-alpha?
Anti-HNF-4-alpha [EPR16885-99] (ab201460) specifically detects a band for HNF-4-alpha (UniProt: P49698) at a molecular weight of 53kDa.
Trusted by the scientific community
Anti-HNF-4-alpha [EPR16885-99] (ab201460) was first used in a scientific publication in 2015 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR16885-99] also available for your convenience: ab201460, Carrier free - ab231167, Alexa Fluor® 555 - ab320111, Alexa Fluor® 594 - ab320112, Alexa Fluor® 647 - ab320113, Alexa Fluor® 488 - ab320698
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HNF-4-alpha influences the expression of genes involved in liver and pancreatic function. It acts as a transcriptional regulator that controls fatty acid oxidation gluconeogenesis and insulin secretion. HNF-4-alpha interacts with other proteins to form transcriptional complexes that fine-tune the expression of metabolic enzymes and transport proteins. This protein is key to ensuring that metabolic genes are expressed in a coordinated manner which is essential for maintaining normal metabolic processes.
Pathways
HNF-4-alpha participates in the insulin signaling and lipid metabolism pathways. Through these pathways it interacts with other transcription factors such as HNF-1-alpha and PPAR-alpha which coordinate the regulation of genes involved in glucose metabolism and fatty acid oxidation. HNF-4-alpha plays a critical role by modulating the expression of enzymes like gluconeogenic enzymes in response to metabolic needs which connects it to key metabolic pathways influencing overall energy homeostasis.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download chicCutRunSequencingBooklet|en
Target data
Publications (37)
Recent publications for all applications. Explore the full list and refine your search
Nature communications 16:7455 PubMed40796541
2025
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Hepatology communications 9: PubMed40366121
2025
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Cell death & disease 16:361 PubMed40325003
2025
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JCI insight 10: PubMed40327401
2025
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iScience 28:111671 PubMed39868030
2025
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Hepatology communications 8: PubMed39761008
2025
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Disease models & mechanisms 17: PubMed39397390
2024
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Scientific reports 14:22927 PubMed39358473
2024
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Cancers 16: PubMed38791872
2024
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Nature communications 15:4034 PubMed38740814
2024
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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