Rabbit Recombinant Monoclonal HNF-4-alpha antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Flow Cytometry (Intracellular) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (AB231167), expandable thumbnail](https://content.abcam.com/products/images/hnf-4-alpha-antibody-epr16885-99-bsa-and-azide-free-ab231167--flow-cytometry-intracellular-img27109.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (AB231167)")
![Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (AB231167), expandable thumbnail](https://content.abcam.com/products/images/hnf-4-alpha-antibody-epr16885-99-bsa-and-azide-free-ab231167--immunocytochemistry-immunofluorescence-img40644.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (AB231167)")
![Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (AB231167), expandable thumbnail](https://content.abcam.com/products/images/hnf-4-alpha-antibody-epr16885-99-bsa-and-azide-free-ab231167--immunocytochemistry-immunofluorescence-img14934.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (AB231167)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (AB231167), expandable thumbnail](https://content.abcam.com/products/images/hnf-4-alpha-antibody-epr16885-99-bsa-and-azide-free-ab231167--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29068.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (AB231167)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (AB231167), expandable thumbnail](https://content.abcam.com/products/images/hnf-4-alpha-antibody-epr16885-99-bsa-and-azide-free-ab231167--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img20368.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (AB231167)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ChIC/CUT&RUN-seq | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected | Expected | Tested |
| Rat | Expected | Expected | Expected | Expected | Tested |
ChIC/CUT&RUN-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes (PubMed:30597922). Activates the transcription of CYP2C38 (By similarity). Represses the CLOCK-BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells (PubMed:30530698).
Alternative names
HNF4, NR2A1, TCF14, HNF4A, Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, Transcription factor HNF-4, TCF-14
Recommended products
Rabbit Recombinant Monoclonal HNF-4-alpha antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR16885-99
- Purification technique
- Affinity purification Protein A
- Specificity
Mouse species are recommended based on IHC result. This antibody is unsuitable to be used in WB on mouse.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab231167 is the carrier-free version of Anti-HNF-4-alpha antibody [EPR16885-99] ab201460.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HNF-4-alpha also known as hepatocyte nuclear factor 4-alpha (HNF4A) is a nuclear protein that functions as a transcription factor. It plays an important role in regulating gene expression in the liver kidney and intestine. HNF-4-alpha has a molecular mass of about 54 kDa and is expressed in tissues where it maintains cellular differentiation and metabolic homeostasis. In cellular assays such as ELISA and CHIP detection of HNF-4-alpha provides insights into its expression levels and activity in various biological contexts.
- Biological function summary
HNF-4-alpha influences the expression of genes involved in liver and pancreatic function. It acts as a transcriptional regulator that controls fatty acid oxidation gluconeogenesis and insulin secretion. HNF-4-alpha interacts with other proteins to form transcriptional complexes that fine-tune the expression of metabolic enzymes and transport proteins. This protein is key to ensuring that metabolic genes are expressed in a coordinated manner which is essential for maintaining normal metabolic processes.
- Pathways
HNF-4-alpha participates in the insulin signaling and lipid metabolism pathways. Through these pathways it interacts with other transcription factors such as HNF-1-alpha and PPAR-alpha which coordinate the regulation of genes involved in glucose metabolism and fatty acid oxidation. HNF-4-alpha plays a critical role by modulating the expression of enzymes like gluconeogenic enzymes in response to metabolic needs which connects it to key metabolic pathways influencing overall energy homeostasis.
- Associated diseases and disorders
HNF-4-alpha is linked to maturity-onset diabetes of the young (MODY1) and certain types of liver diseases. Mutations or dysregulation in HNF-4-alpha can affect the expression of genes essential for insulin production and glucose metabolism leading to disorders like diabetes. Additionally HNF-4-alpha interacts with other proteins and transcription factors implicated in these conditions such as HNF-1-alpha. Understanding the role of HNF-4-alpha in these diseases can provide insights into potential therapeutic targets and intervention strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Flow Cytometry (Intracellular) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167), expandable thumbnail](https://content.abcam.com/products/images/hnf-4-alpha-antibody-epr16885-99-bsa-and-azide-free-ab231167--flow-cytometry-intracellular-img27109.jpg "Flow Cytometry (Intracellular) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167)")
Flow Cytometry (Intracellular) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167)
Intracellular Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) labelling CDKN2A/p16INK4a with purified Anti-HNF-4-alpha antibody [EPR16885-99] ab201460 at 1/1000 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HNF-4-alpha antibody [EPR16885-99] ab201460).
![Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167), expandable thumbnail](https://content.abcam.com/products/images/hnf-4-alpha-antibody-epr16885-99-bsa-and-azide-free-ab231167--immunocytochemistry-immunofluorescence-img40644.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167)")
Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton-X100 permeabilized HT-29 (Human colorectal adenocarcinoma cells) cells labeling HNF-4-alpha with Anti-HNF-4-alpha antibody [EPR16885-99] ab201460 at 1/2000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/500 dilution (green).
Confocal image showing nuclear staining on HT-29 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is stained with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/500 dilution (red).
-ve control 1: Anti-HNF-4-alpha antibody [EPR16885-99] ab201460 at 1/2000 dilution followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/500 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HNF-4-alpha antibody [EPR16885-99] ab201460).
![Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167), expandable thumbnail](https://content.abcam.com/products/images/hnf-4-alpha-antibody-epr16885-99-bsa-and-azide-free-ab231167--immunocytochemistry-immunofluorescence-img14934.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167)")
Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton-X100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling HNF-4-alpha with Anti-HNF-4-alpha antibody [EPR16885-99] ab201460 at 1/2000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/500 dilution (green).
Confocal image showing nuclear staining on HT-29 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is stained with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/500 dilution (red).
-ve control 1: Anti-HNF-4-alpha antibody [EPR16885-99] ab201460 at 1/2000 dilution followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/500 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HNF-4-alpha antibody [EPR16885-99] ab201460).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167), expandable thumbnail](https://content.abcam.com/products/images/hnf-4-alpha-antibody-epr16885-99-bsa-and-azide-free-ab231167--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29068.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167)
This IHC data was generated using the same anti-HNF4 antibody clone, EPR16885-99, in a different buffer formulation (cat# Anti-HNF-4-alpha antibody [EPR16885-99] ab201460).
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HNF-4-alpha with Anti-HNF-4-alpha antibody [EPR16885-99] ab201460 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on Human colon tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167), expandable thumbnail](https://content.abcam.com/products/images/hnf-4-alpha-antibody-epr16885-99-bsa-and-azide-free-ab231167--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img20368.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HNF-4-alpha with Anti-HNF-4-alpha antibody [EPR16885-99] ab201460 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on Human liver tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HNF-4-alpha antibody [EPR16885-99] ab201460).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167), expandable thumbnail](https://content.abcam.com/products/images/hnf-4-alpha-antibody-epr16885-99-bsa-and-azide-free-ab231167--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61126.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling HNF-4-alpha with Anti-HNF-4-alpha antibody [EPR16885-99] ab201460 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on mouse liver tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HNF-4-alpha antibody [EPR16885-99] ab201460).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167), expandable thumbnail](https://content.abcam.com/products/images/hnf-4-alpha-antibody-epr16885-99-bsa-and-azide-free-ab231167--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96267.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167)
This IHC data was generated using the same anti-HNF4 antibody clone, EPR16885-99, in a different buffer formulation (cat# Anti-HNF-4-alpha antibody [EPR16885-99] ab201460).
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling HNF-4-alpha with Anti-HNF-4-alpha antibody [EPR16885-99] ab201460 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on rat colon tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![ChIC/CUT&RUN sequencing - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167), expandable thumbnail](https://content.abcam.com/products/images/hnf-4-alpha-antibody-epr16885-99-bsa-and-azide-free-ab231167--chic-cut-run-sequencing-img420240.jpg "ChIC/CUT&RUN sequencing - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167)")
ChIC/CUT&RUN sequencing - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167)
ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 HepG2 (Human liver hepatocellular carcinoma cell line) cells and 5 µg of Anti-HNF-4-alpha antibody [EPR16885-99] ab201460 [EPR16885-99)]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
Additional screenshots of mapped reads can be found in the Protocol booklet in the Support and downloads section.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HNF-4-alpha antibody [EPR16885-99] ab201460).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167), expandable thumbnail](https://content.abcam.com/products/images/hnf-4-alpha-antibody-epr16885-99-bsa-and-azide-free-ab231167--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61126.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167), expandable thumbnail](https://content.abcam.com/products/images/hnf-4-alpha-antibody-epr16885-99-bsa-and-azide-free-ab231167--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96267.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167)")
![ChIC/CUT&RUN sequencing - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167), expandable thumbnail](https://content.abcam.com/products/images/hnf-4-alpha-antibody-epr16885-99-bsa-and-azide-free-ab231167--chic-cut-run-sequencing-img420240.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-HNF-4-alpha antibody [EPR16885-99] - BSA and Azide free (ab231167)")
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