AB174653

Anti-HNF1 alpha antibody

Name: Anti-HNF1 alpha antibody (ab174653) | Abcam
Brand: Abcam Limited
SKU: AB174653
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal HNF1 alpha antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human HNF1A aa 550-600.

View Alternative Names

TCF1, HNF1A, Hepatocyte nuclear factor 1-alpha, HNF-1-alpha, HNF-1A, Liver-specific transcription factor LF-B1, Transcription factor 1, LFB1, TCF-1

2 Images

Supplier Data

Immunoprecipitation - Anti-HNF1 alpha antibody (AB174653)

Detection of HNF1 by Western Blot of Immunprecipitate.
ab174653 at 1μg/ml labeling HNF1 in HepG2 whole cell lysate immunoprecipitated using ab174653 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

All lanes:

Immunoprecipitation - Anti-HNF1 alpha antibody (ab174653)

Predicted band size: 67 kDa

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Exposure time: 3s

Supplier Data

Western blot - Anti-HNF1 alpha antibody (AB174653)

All lanes:

Western blot - Anti-HNF1 alpha antibody (ab174653) at 0.1 µg/mL

All lanes:

HepG2 whole cell lysate at 50 µg

Predicted band size: 67 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human HNF1A aa 550-600. The exact immunogen used to generate this antibody is proprietary information.

P20823

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab174653 is affinity purified using an epitope specific to HNF1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HNF1 alpha also known as hepatocyte nuclear factor 1 alpha is a transcription factor that has an important role in regulating gene expression. It has a mass of approximately 67 kDa. Predominantly HNF1 alpha appears in the liver kidney intestines and pancreas. It binds to enhancer elements of target genes affecting the transcriptional activity. The protein is also vital for cellular differentiation and maintenance in tissues where it is expressed.

Biological function summary

The HNF1 alpha functions as part of the dimeric HNF1 protein complex often partnering with HNF1 beta. It governs a wide array of genes including those involved in carbohydrate and lipid metabolism. By influencing the gene expression HNF1 alpha assists in maintaining normal metabolic processes. Its activity is critical for proper liver function and insulin secretion from pancreatic beta cells.

Pathways

HNF1 alpha actively integrates into key metabolic pathways such as the gluconeogenesis and the cholesterol biosynthesis pathways. In these pathways it works alongside other proteins like HNF4 alpha and PGC-1 alpha contributing significantly to glucose and lipid homeostasis. The transcription factor impacts the metabolic pathway reinforcing its integral role in maintaining energy balance and metabolic health.

HNF1 alpha is importantly linked to maturity-onset diabetes of the young (MODY) and certain forms of liver disease. Mutations or dysregulation in HNF1 alpha can cause MODY3 a subtype of diabetes characterized by an autosomal dominant pattern of inheritance. Additionally the abnormal activity of HNF1 alpha may contribute to liver dysfunction often in conjunction with HNF4 alpha disruptions. These associations highlight the importance of HNF1 alpha in both metabolic pathway integrity and disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver (By similarity). Binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed : 10966642, PubMed : 12453420). Activates the transcription of CYP1A2, CYP2E1 and CYP3A11 (By similarity).. (Microbial infection) Plays a crucial role for hepatitis B virus gene transcription and DNA replication. Mechanistically, synergistically cooperates with NR5A2 to up-regulate the activity of one of the critical cis-elements in the hepatitis B virus genome enhancer II (ENII).

See full target information HNF1A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Drug metabolism and disposition: the biological fate of chemicals 49:361-368 PubMed33674270

2021

The Long Noncoding RNA Hepatocyte Nuclear Factor 4 Antisense RNA 1 Negatively Regulates Cytochrome P450 Enzymes in Huh7 Cells via Histone Modifications.

Applications

Unspecified application

Species

Unspecified reactive species

Pei Wang,Shitong Chen,Yiting Wang,Xiaofei Wang,Liang Yan,Kun Yang,Xiao-Bo Zhong,Shengna Han,Lirong Zhang

The Journal of pharmacology and experimental thera 368:353-362 PubMed30602592

2019

The HNF1-Regulated LncRNA HNF1-AS1 Is Involved in the Regulation of Cytochrome P450 Expression in Human Liver Tissues and Huh7 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yiting Wang,Liang Yan,Jingyang Liu,Shitong Chen,Guangming Liu,Yali Nie,Pei Wang,Weihong Yang,Liming Chen,Xiaobo Zhong,Shengna Han,Lirong Zhang

Drug metabolism and disposition: the biological fa 45:1372-1378 PubMed29025858

2017

Histone Modifications Regulate the Developmental Expression of Human Hepatic UDP-Glucuronosyltransferase 1A1.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Li Nie,Xiang-Guang Meng,Jing-Yang Liu,Liang Yan,Pei Wang,Hong-Zheng Bi,Quan-Cheng Kan,Li-Rong Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HNF1 alpha antibody

Immunoprecipitation - Anti-HNF1 alpha antibody (AB174653)

Western blot - Anti-HNF1 alpha antibody (AB174653)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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