Rabbit Polyclonal HNF1 alpha antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human HNF1A aa 550-600.
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- Drug metabolism and disposition: the biological fate of chemicals 49:361-3682021The Long Noncoding RNA Hepatocyte Nuclear Factor 4 Antisense RNA 1 Negatively Regulates Cytochrome P450 Enzymes in Huh7 Cells via Histone Modifications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPei Wang,Shitong Chen,Yiting Wang,Xiaofei Wang,Liang Yan,Kun Yang,Xiao-Bo Zhong,Shengna Han,Lirong ZhangPubMed 33674270
- The Journal of pharmacology and experimental thera 368:353-3622019The HNF1-Regulated LncRNA HNF1-AS1 Is Involved in the Regulation of Cytochrome P450 Expression in Human Liver Tissues and Huh7 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiting Wang et. al.PubMed 30602592
- Drug metabolism and disposition: the biological fa 45:1372-13782017Histone Modifications Regulate the Developmental Expression of Human Hepatic UDP-Glucuronosyltransferase 1A1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYa-Li Nie et. al.PubMed 29025858
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human HNF1A aa 550-600. The exact immunogen used to generate this antibody is proprietary information. Database link P20823
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Associated Products
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Target data
Function
Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver (By similarity). Binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed:10966642, PubMed:12453420). Activates the transcription of CYP1A2, CYP2E1 and CYP3A11 (By similarity). (Microbial infection) Plays a crucial role for hepatitis B virus gene transcription and DNA replication. Mechanistically, synergistically cooperates with NR5A2 to up-regulate the activity of one of the critical cis-elements in the hepatitis B virus genome enhancer II (ENII).
Alternative names
TCF1, HNF1A, Hepatocyte nuclear factor 1-alpha, HNF-1-alpha, HNF-1A, Liver-specific transcription factor LF-B1, Transcription factor 1, LFB1, TCF-1
Recommended products
Rabbit Polyclonal HNF1 alpha antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human HNF1A aa 550-600.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human HNF1A aa 550-600. The exact immunogen used to generate this antibody is proprietary information. Database link P20823
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab174653 is affinity purified using an epitope specific to HNF1 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The HNF1 alpha also known as hepatocyte nuclear factor 1 alpha is a transcription factor that has an important role in regulating gene expression. It has a mass of approximately 67 kDa. Predominantly HNF1 alpha appears in the liver kidney intestines and pancreas. It binds to enhancer elements of target genes affecting the transcriptional activity. The protein is also vital for cellular differentiation and maintenance in tissues where it is expressed.
- Biological function summary
The HNF1 alpha functions as part of the dimeric HNF1 protein complex often partnering with HNF1 beta. It governs a wide array of genes including those involved in carbohydrate and lipid metabolism. By influencing the gene expression HNF1 alpha assists in maintaining normal metabolic processes. Its activity is critical for proper liver function and insulin secretion from pancreatic beta cells.
- Pathways
HNF1 alpha actively integrates into key metabolic pathways such as the gluconeogenesis and the cholesterol biosynthesis pathways. In these pathways it works alongside other proteins like HNF4 alpha and PGC-1 alpha contributing significantly to glucose and lipid homeostasis. The transcription factor impacts the metabolic pathway reinforcing its integral role in maintaining energy balance and metabolic health.
- Associated diseases and disorders
HNF1 alpha is importantly linked to maturity-onset diabetes of the young (MODY) and certain forms of liver disease. Mutations or dysregulation in HNF1 alpha can cause MODY3 a subtype of diabetes characterized by an autosomal dominant pattern of inheritance. Additionally the abnormal activity of HNF1 alpha may contribute to liver dysfunction often in conjunction with HNF4 alpha disruptions. These associations highlight the importance of HNF1 alpha in both metabolic pathway integrity and disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunoprecipitation - Anti-HNF1 alpha antibody (ab174653)
Detection of HNF1 by Western Blot of Immunprecipitate.
ab174653 at 1μg/ml labeling HNF1 in HepG2 whole cell lysate immunoprecipitated using ab174653 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).All lanes: Immunoprecipitation - Anti-HNF1 alpha antibody (ab174653)
Developed using the ECL technique.
Predicted band size: 67 kDa
Exposure time: 3s
Western blot - Anti-HNF1 alpha antibody (ab174653)
All lanes: Western blot - Anti-HNF1 alpha antibody (ab174653) at 0.1 µg/mL
All lanes: HepG2 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 67 kDa
Exposure time: 30s
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