AB204306

Anti-HNF1 alpha antibody

Name: Anti-HNF1 alpha antibody (ab204306) | Abcam
Brand: Abcam Limited
SKU: AB204306
Rating: 5 (1 reviews)

(1 Review)

(8 Publications)

Rabbit Polyclonal HNF1 alpha antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HNF1A aa 250-350.

View Alternative Names

TCF1, HNF1A, Hepatocyte nuclear factor 1-alpha, HNF-1-alpha, HNF-1A, Liver-specific transcription factor LF-B1, Transcription factor 1, LFB1, TCF-1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-HNF1 alpha antibody (AB204306)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HNF1 alpha antibody (AB204306)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized CACO-2 cells labeling HNF1 with ab204306 at 4 μg/mL (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody (AB204306)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody (AB204306)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling HNF1 with ab204306 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody (AB204306)

Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling HNF1 with ab204306 at a 1/200 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human HNF1A aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

P20823

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HNF1 alpha also known as hepatocyte nuclear factor 1 alpha is a transcription factor that has an important role in regulating gene expression. It has a mass of approximately 67 kDa. Predominantly HNF1 alpha appears in the liver kidney intestines and pancreas. It binds to enhancer elements of target genes affecting the transcriptional activity. The protein is also vital for cellular differentiation and maintenance in tissues where it is expressed.

Biological function summary

The HNF1 alpha functions as part of the dimeric HNF1 protein complex often partnering with HNF1 beta. It governs a wide array of genes including those involved in carbohydrate and lipid metabolism. By influencing the gene expression HNF1 alpha assists in maintaining normal metabolic processes. Its activity is critical for proper liver function and insulin secretion from pancreatic beta cells.

Pathways

HNF1 alpha actively integrates into key metabolic pathways such as the gluconeogenesis and the cholesterol biosynthesis pathways. In these pathways it works alongside other proteins like HNF4 alpha and PGC-1 alpha contributing significantly to glucose and lipid homeostasis. The transcription factor impacts the metabolic pathway reinforcing its integral role in maintaining energy balance and metabolic health.

HNF1 alpha is importantly linked to maturity-onset diabetes of the young (MODY) and certain forms of liver disease. Mutations or dysregulation in HNF1 alpha can cause MODY3 a subtype of diabetes characterized by an autosomal dominant pattern of inheritance. Additionally the abnormal activity of HNF1 alpha may contribute to liver dysfunction often in conjunction with HNF4 alpha disruptions. These associations highlight the importance of HNF1 alpha in both metabolic pathway integrity and disease pathology.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver (By similarity). Binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed : 10966642, PubMed : 12453420). Activates the transcription of CYP1A2, CYP2E1 and CYP3A11 (By similarity).. (Microbial infection) Plays a crucial role for hepatitis B virus gene transcription and DNA replication. Mechanistically, synergistically cooperates with NR5A2 to up-regulate the activity of one of the critical cis-elements in the hepatitis B virus genome enhancer II (ENII).

See full target information HNF1A

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

The International journal of developmental biology 68:223-230 PubMed38270262

2024

The GLI code controls HNF1A levels during foregut differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Lucas Unger,Andreas F Mathisen,Simona Chera,Thomas Aga Legøy,Luiza Ghila

Nature communications 14:5400 PubMed37669939

2023

Single cell multiomic analysis reveals diabetes-associated β-cell heterogeneity driven by HNF1A.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Weng,Anniya Gu,Shanshan Zhang,Leina Lu,Luxin Ke,Peidong Gao,Xiaoxiao Liu,Yuntong Wang,Peinan Hu,Dylan Plummer,Elise MacDonald,Saixian Zhang,Jiajia Xi,Sisi Lai,Konstantin Leskov,Kyle Yuan,Fulai Jin,Yan Li

Nature communications 12:3133 PubMed34035238

2021

Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells.

Applications

Unspecified application

Species

Unspecified reactive species

Blaise Su Jun Low,Chang Siang Lim,Shirley Suet Lee Ding,Yaw Sing Tan,Natasha Hui Jin Ng,Vidhya Gomathi Krishnan,Su Fen Ang,Claire Wen Ying Neo,Chandra S Verma,Shawn Hoon,Su Chi Lim,E Shyong Tai,Adrian Kee Keong Teo

Scientific reports 11:10363 PubMed33990627

2021

HNF1A regulates colorectal cancer progression and drug resistance as a downstream of POU5F1.

Applications

Unspecified application

Species

Unspecified reactive species

Shiki Fujino,Norikatsu Miyoshi,Aya Ito,Masayoshi Yasui,Chu Matsuda,Masayuki Ohue,Mamoru Uemura,Tsunekazu Mizushima,Yuichiro Doki,Hidetoshi Eguchi

The EMBO journal 39:e102808 PubMed32154941

2020

HNF1A recruits KDM6A to activate differentiated acinar cell programs that suppress pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mark Kalisz,Edgar Bernardo,Anthony Beucher,Miguel Angel Maestro,Natalia Del Pozo,Irene Millán,Lena Haeberle,Martin Schlensog,Sami Alexander Safi,Wolfram Trudo Knoefel,Vanessa Grau,Matías de Vas,Karl B Shpargel,Eva Vaquero,Terry Magnuson,Sagrario Ortega,Irene Esposito,Francisco X Real,Jorge Ferrer

Journal of Cancer 11:940-948 PubMed31949497

2020

LNC00673 suppresses proliferation and metastasis of pancreatic cancer via target miR-504/ HNF1A.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Gong,Hai-Su Dai,Jun-Jie Shu,Wei Liu,Ping Bie,Lei-da Zhang

Journal of cellular biochemistry 120:2816-2827 PubMed28865121

2017

HNF1A/CASC2 regulates pancreatic cancer cell proliferation through PTEN/Akt signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqun Yu,Shuai Liang,Yingqiong Zhou,Shuqun Li,Yixiong Li,Weijia Liao

Oncotarget 7:38440-38450 PubMed27469031

2016

Validation of a multi-omics strategy for prioritizing personalized candidate driver genes

Applications

Unspecified application

Species

Unspecified reactive species

Li Liang,Liting Song,Yi Yang,Ling Tian,Xiaoyuan Li,Songfeng Wu,Wenxun Huang,Hong Ren,Ni Tang,Keyue Ding

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HNF1 alpha antibody

Immunocytochemistry/ Immunofluorescence - Anti-HNF1 alpha antibody (AB204306)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody (AB204306)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody (AB204306)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise