Rabbit Polyclonal HNF1 alpha antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HNF1A aa 250-350.
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- Nature communications 14:54002023Single cell multiomic analysis reveals diabetes-associated β-cell heterogeneity driven by HNF1A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Weng,Anniya Gu,Shanshan Zhang,Leina Lu,Luxin Ke,Peidong Gao,Xiaoxiao Liu,Yuntong Wang,Peinan Hu,Dylan Plummer,Elise MacDonald,Saixian Zhang,Jiajia Xi,Sisi Lai,Konstantin Leskov,Kyle Yuan,Fulai Jin,Yan LiPubMed 37669939
- Nature communications 12:31332021Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBlaise Su Jun Low,Chang Siang Lim,Shirley Suet Lee Ding,Yaw Sing Tan,Natasha Hui Jin Ng,Vidhya Gomathi Krishnan,Su Fen Ang,Claire Wen Ying Neo,Chandra S Verma,Shawn Hoon,Su Chi Lim,E Shyong Tai,Adrian Kee Keong TeoPubMed 34035238
- Scientific reports 11:103632021HNF1A regulates colorectal cancer progression and drug resistance as a downstream of POU5F1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiki Fujino,Norikatsu Miyoshi,Aya Ito,Masayoshi Yasui,Chu Matsuda,Masayuki Ohue,Mamoru Uemura,Tsunekazu Mizushima,Yuichiro Doki,Hidetoshi EguchiPubMed 33990627
- The EMBO journal 39:e1028082020HNF1A recruits KDM6A to activate differentiated acinar cell programs that suppress pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMark Kalisz et. al.PubMed 32154941
- Journal of Cancer 11:940-9482020LNC00673 suppresses proliferation and metastasis of pancreatic cancer via target miR-504/ HNF1A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Gong et. al.PubMed 31949497
- Journal of cellular biochemistry 120:2816-28272017HNF1A/CASC2 regulates pancreatic cancer cell proliferation through PTEN/Akt signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaqun Yu,Shuai Liang,Yingqiong Zhou,Shuqun Li,Yixiong Li,Weijia LiaoPubMed 28865121
- Oncotarget 7:38440-384502016Validation of a multi-omics strategy for prioritizing personalized candidate driver genesApplications:Unspecified applicationReactive species:Unspecified reactive speciesLi Liang,Liting Song,Yi Yang,Ling Tian,Xiaoyuan Li,Songfeng Wu,Wenxun Huang,Hong Ren,Ni Tang,Keyue DingPubMed 27469031
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HNF1A aa 250-350. The exact immunogen used to generate this antibody is proprietary information. Database link P20823
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Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 - 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25-2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver (By similarity). Binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed:10966642, PubMed:12453420). Activates the transcription of CYP1A2, CYP2E1 and CYP3A11 (By similarity). (Microbial infection) Plays a crucial role for hepatitis B virus gene transcription and DNA replication. Mechanistically, synergistically cooperates with NR5A2 to up-regulate the activity of one of the critical cis-elements in the hepatitis B virus genome enhancer II (ENII).
Alternative names
TCF1, HNF1A, Hepatocyte nuclear factor 1-alpha, HNF-1-alpha, HNF-1A, Liver-specific transcription factor LF-B1, Transcription factor 1, LFB1, TCF-1
Recommended products
Rabbit Polyclonal HNF1 alpha antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HNF1A aa 250-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HNF1A aa 250-350. The exact immunogen used to generate this antibody is proprietary information. Database link P20823
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The HNF1 alpha also known as hepatocyte nuclear factor 1 alpha is a transcription factor that has an important role in regulating gene expression. It has a mass of approximately 67 kDa. Predominantly HNF1 alpha appears in the liver kidney intestines and pancreas. It binds to enhancer elements of target genes affecting the transcriptional activity. The protein is also vital for cellular differentiation and maintenance in tissues where it is expressed.
- Biological function summary
The HNF1 alpha functions as part of the dimeric HNF1 protein complex often partnering with HNF1 beta. It governs a wide array of genes including those involved in carbohydrate and lipid metabolism. By influencing the gene expression HNF1 alpha assists in maintaining normal metabolic processes. Its activity is critical for proper liver function and insulin secretion from pancreatic beta cells.
- Pathways
HNF1 alpha actively integrates into key metabolic pathways such as the gluconeogenesis and the cholesterol biosynthesis pathways. In these pathways it works alongside other proteins like HNF4 alpha and PGC-1 alpha contributing significantly to glucose and lipid homeostasis. The transcription factor impacts the metabolic pathway reinforcing its integral role in maintaining energy balance and metabolic health.
- Associated diseases and disorders
HNF1 alpha is importantly linked to maturity-onset diabetes of the young (MODY) and certain forms of liver disease. Mutations or dysregulation in HNF1 alpha can cause MODY3 a subtype of diabetes characterized by an autosomal dominant pattern of inheritance. Additionally the abnormal activity of HNF1 alpha may contribute to liver dysfunction often in conjunction with HNF4 alpha disruptions. These associations highlight the importance of HNF1 alpha in both metabolic pathway integrity and disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody (ab204306)
Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling HNF1 with ab204306 at a 1/200 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-HNF1 alpha antibody (ab204306)
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized CACO-2 cells labeling HNF1 with ab204306 at 4 μg/mL (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody (ab204306)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling HNF1 with ab204306 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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