Rabbit Polyclonal HNF1 alpha antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 15 publications.
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- Osteoarthritis and cartilage 31:507-5182022Hypoxia and Wnt signaling inversely regulate expression of chondroprotective molecule ANP32A in articular cartilage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ Quintiens,A De Roover,F M F Cornelis,A Escribano-Núñez,A Sermon,S Pazmino,S Monteagudo,R J LoriesPubMed 36370958
- Nature communications 12:31332021Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBlaise Su Jun Low,Chang Siang Lim,Shirley Suet Lee Ding,Yaw Sing Tan,Natasha Hui Jin Ng,Vidhya Gomathi Krishnan,Su Fen Ang,Claire Wen Ying Neo,Chandra S Verma,Shawn Hoon,Su Chi Lim,E Shyong Tai,Adrian Kee Keong TeoPubMed 34035238
- Frontiers in pharmacology 11:6059672021The Role of Bone Morphogenetic Protein 9 in Nonalcoholic Fatty Liver Disease in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin-Juan Sun,Ling-Yan Cai,Jie Jian,Ya-Lu Cui,Chen-Kai Huang,Shu-Qing Liu,Jin-Lai Lu,Wei Wang,Xin Zeng,Lan ZhongPubMed 33603666
- OncoTargets and therapy 13:7641-76522020HNF1A-Induced lncRNA HCG18 Facilitates Gastric Cancer Progression by Upregulating DNAJB12 via miR-152-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPei Ma et. al.PubMed 32801777
- Cell reports 31:1076552020Generation and Profiling of 2,135 Human ESC Lines for the Systematic Analyses of Cell States Perturbed by Inducing Single Transcription Factors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuhki Nakatake,Shigeru B H Ko,Alexei A Sharov,Shunichi Wakabayashi,Miyako Murakami,Miki Sakota,Nana Chikazawa,Chiaki Ookura,Saeko Sato,Noriko Ito,Madoka Ishikawa-Hirayama,Siu Shan Mak,Lars Martin Jakt,Tomoo Ueno,Ken Hiratsuka,Misako Matsushita,Sravan Kumar Goparaju,Tomohiko Akiyama,Kei-Ichiro Ishiguro,Mayumi Oda,Norio Gouda,Akihiro Umezawa,Hidenori Akutsu,Kunihiro Nishimura,Ryo Matoba,Osamu Ohara,Minoru S H KoPubMed 32433964
- Diabetes, metabolic syndrome and obesity : targets 13:627-6392020Loss of HNF1α Function Contributes to Hepatocyte Proliferation and Abnormal Cholesterol Metabolism via Downregulating miR-122: A Novel Mechanism of MODY3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengdie Hu et. al.PubMed 32184642
- Medical science monitor : international medical jo 26:e9185282020Romidepsin (FK228) in a Mouse Model of Lipopolysaccharide-Induced Acute Kidney Injury is Associated with Down-Regulation of the CYP2E1 Gene.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShulin Cheng et. al.PubMed 31954012
- EBioMedicine 44:403-4182019HNF1A inhibition induces the resistance of pancreatic cancer cells to gemcitabine by targeting ABCB1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanan Lu et. al.PubMed 31103629
- Journal of diabetes research 2019:54839462019HNF1 Controls Liver Lipid Metabolism and Insulin Resistance via Negatively Regulating the SOCS-3-STAT3 Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaorong Tan et. al.PubMed 31223625
- Scientific reports 9:9132019Induction of human pluripotent stem cells into kidney tissues by synthetic mRNAs encoding transcription factors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKen Hiratsuka et. al.PubMed 30696889
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Expected |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes Lysates should be made freshly and used in WB immediately to minimize protein degradation. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig, Cow | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes Recommend using PFA fixation instead of Methanol fixation. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig, Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver (By similarity). Binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed:10966642, PubMed:12453420). Activates the transcription of CYP1A2, CYP2E1 and CYP3A11 (By similarity). (Microbial infection) Plays a crucial role for hepatitis B virus gene transcription and DNA replication. Mechanistically, synergistically cooperates with NR5A2 to up-regulate the activity of one of the critical cis-elements in the hepatitis B virus genome enhancer II (ENII).
Alternative names
TCF1, HNF1A, Hepatocyte nuclear factor 1-alpha, HNF-1-alpha, HNF-1A, Liver-specific transcription factor LF-B1, Transcription factor 1, LFB1, TCF-1
Recommended products
Rabbit Polyclonal HNF1 alpha antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 15 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified by antigen-affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The HNF1 alpha also known as hepatocyte nuclear factor 1 alpha is a transcription factor that has an important role in regulating gene expression. It has a mass of approximately 67 kDa. Predominantly HNF1 alpha appears in the liver kidney intestines and pancreas. It binds to enhancer elements of target genes affecting the transcriptional activity. The protein is also vital for cellular differentiation and maintenance in tissues where it is expressed.
- Biological function summary
The HNF1 alpha functions as part of the dimeric HNF1 protein complex often partnering with HNF1 beta. It governs a wide array of genes including those involved in carbohydrate and lipid metabolism. By influencing the gene expression HNF1 alpha assists in maintaining normal metabolic processes. Its activity is critical for proper liver function and insulin secretion from pancreatic beta cells.
- Pathways
HNF1 alpha actively integrates into key metabolic pathways such as the gluconeogenesis and the cholesterol biosynthesis pathways. In these pathways it works alongside other proteins like HNF4 alpha and PGC-1 alpha contributing significantly to glucose and lipid homeostasis. The transcription factor impacts the metabolic pathway reinforcing its integral role in maintaining energy balance and metabolic health.
- Associated diseases and disorders
HNF1 alpha is importantly linked to maturity-onset diabetes of the young (MODY) and certain forms of liver disease. Mutations or dysregulation in HNF1 alpha can cause MODY3 a subtype of diabetes characterized by an autosomal dominant pattern of inheritance. Additionally the abnormal activity of HNF1 alpha may contribute to liver dysfunction often in conjunction with HNF4 alpha disruptions. These associations highlight the importance of HNF1 alpha in both metabolic pathway integrity and disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-HNF1 alpha antibody (ab96777)
7.5% SDS PAGE
Blocking: 5% non-fat milk in TBST, room temperature, 60 minutes.
Lysates should be made freshly and used in WB immediately to minimize protein degradation. The molecular weight observed is consistent with what has been described in the literature (PMID:31145732).
Negative control: HeLa (PMID: 12488960). Also, 293T and A431.
All lanes: Western blot - Anti-HNF1 alpha antibody (ab96777) at 1/5000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 67 kDa
Observed band size: 81 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody (ab96777)
ab96777, at a 1/100 dilution, staining HNF1 alpha in paraffin embedded A549 Xenograft by Immunohistochemistry.
Western blot - Anti-HNF1 alpha antibody (ab96777)
Western blot: Anti-HNF1A antibody (ab96777) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab96777 was shown to bind specifically to HNF1A. A band was observed at 75-80 kDa in wild-type A549 cell lysates with no signal observed at this size in HNF1A knockout cell line. To generate this image, wild-type and HNF1A knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-HNF1 alpha antibody (ab96777) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: HNF1A knockout A549 cell lysate at 20 µg
Lane 3: HepG2 cell lysate at 20 µg
Lane 4: U-2 OS cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 75 kDa, 80 kDa
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