Anti-HNF1 alpha antibody [EPR23054-142]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [EPR23054-142] (AB242140)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling HNF1A with ab242140 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human pancreas (PMID : 25793983) is observed. The section was incubated with ab242140 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [EPR23054-142] (AB242140)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HNF1A with ab242140 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human liver (PMID : 26169608) is observed. The section was incubated with ab242140 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• WB

Lab

Western blot - Anti-HNF1 alpha antibody [EPR23054-142] (AB242140)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates should be made freshly and used in WB immediately to minimize protein degradation. The molecular weight observed is consistent with what has been described in the literature (PMID : 31145732). Exposure time : 6 seconds

All lanes:

Western blot - Anti-HNF1 alpha antibody [EPR23054-142] (ab242140) at 1/1000 dilution

All lanes:

Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 67 kDa

Observed band size: 81 kDa

false

• WB

Lab

Western blot - Anti-HNF1 alpha antibody [EPR23054-142] (AB242140)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Lysates should be made freshly and used in WB immediately to minimize protein degradation. The molecular weight observed is consistent with what has been described in the literature (PMID : 31145732).

Negative control : HeLa (PMID : 12488960)

Exposure time : 5.5 seconds

All lanes:

Western blot - Anti-HNF1 alpha antibody [EPR23054-142] (ab242140) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 67 kDa

Observed band size: 81 kDa

false

• ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Anti-HNF1 alpha antibody [EPR23054-142] (AB242140)

ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 HepG2 (Human liver hepatocellular carcinoma cell line) cells and 5µg of ab242140 [EPR23054-142]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. Additional screenshots of mapped reads can be found in the Protocol booklet in the Product Protocol section. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.