Anti-HNF1 alpha antibody [HNF1A/2087] - BSA and Azide free
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Mouse Monoclonal HNF1 alpha antibody. Carrier free. Suitable for Protein Array, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HNF1A aa 200-350.
View Alternative Names
TCF1, HNF1A, Hepatocyte nuclear factor 1-alpha, HNF-1-alpha, HNF-1A, Liver-specific transcription factor LF-B1, Transcription factor 1, LFB1, TCF-1
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [HNF1A/2087] - BSA and Azide free (AB268239)
Formalin-fixed, paraffin-embedded human small intestine tissue stained for HNF1 alpha using ab268116 at 2 μg/ml in immunohistochemical analysis.
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268116).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [HNF1A/2087] - BSA and Azide free (AB268239)
Formalin-fixed, paraffin-embedded human pancreas tissue stained for HNF1 alpha using ab268116 at 2 μg/ml in immunohistochemical analysis.
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268116).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [HNF1A/2087] - BSA and Azide free (AB268239)
Formalin-fixed, paraffin-embedded human pancreas tissue stained for HNF1 alpha using ab268116 at 2 μg/ml in immunohistochemical analysis.
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268116).
- Protein Array
Unknown
Protein Array - Anti-HNF1 alpha antibody [HNF1A/2087] - BSA and Azide free (AB268239)
Analysis of a Protein Array containing more than 19,000 full-length human proteins using ab268116.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268116).
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab2682116)
Reactivity data
Product details
ab268239 is the carrier-free version of ab268116.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The HNF1 alpha functions as part of the dimeric HNF1 protein complex often partnering with HNF1 beta. It governs a wide array of genes including those involved in carbohydrate and lipid metabolism. By influencing the gene expression HNF1 alpha assists in maintaining normal metabolic processes. Its activity is critical for proper liver function and insulin secretion from pancreatic beta cells.
Pathways
HNF1 alpha actively integrates into key metabolic pathways such as the gluconeogenesis and the cholesterol biosynthesis pathways. In these pathways it works alongside other proteins like HNF4 alpha and PGC-1 alpha contributing significantly to glucose and lipid homeostasis. The transcription factor impacts the metabolic pathway reinforcing its integral role in maintaining energy balance and metabolic health.
Product protocols
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Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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