Anti-HNF1 alpha antibody [RM1094] (ab316870)

Rabbit Recombinant Multiclonal HNF1 alpha antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), ChIC/CUT&RUN-seq, IP and reacts with Human, Mouse, Rat samples.

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Images

ChIC/CUT&RUN sequencing - Anti-HNF1 alpha antibody [RM1094] (AB316870), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Multiclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF	Flow Cyt (Intra)	ChIC/CUT&RUN-seq	IP
Human	Tested	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Not recommended	Not recommended	Not recommended	Expected	Expected
Rat	Tested	Not recommended	Expected	Expected	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 20 µg for 10⁶ Cells	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information HNF1A

Function

Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver (By similarity). Binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed:10966642, PubMed:12453420). Activates the transcription of CYP1A2, CYP2E1 and CYP3A11 (By similarity).(Microbial infection) Plays a crucial role for hepatitis B virus gene transcription and DNA replication. Mechanistically, synergistically cooperates with NR5A2 to up-regulate the activity of one of the critical cis-elements in the hepatitis B virus genome enhancer II (ENII).

Alternative names

TCF1, HNF1A, Hepatocyte nuclear factor 1-alpha, HNF-1-alpha, HNF-1A, Liver-specific transcription factor LF-B1, Transcription factor 1, LFB1, TCF-1

Recommended products

Rabbit Recombinant Multiclonal HNF1 alpha antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), ChIC/CUT&RUN-seq, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Multiclonal

Immunogens

The exact immunogen used to generate this antibody is proprietary information.

Clone number

RM1094

Purification technique

Affinity purification Protein A

Specificity

Unsuitable for mouse and rat IHC.

Unsuitable for mouse ICC and mouse FC.

Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

This product is a recombinant multiclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary: The HNF1 alpha also known as hepatocyte nuclear factor 1 alpha is a transcription factor that has an important role in regulating gene expression. It has a mass of approximately 67 kDa. Predominantly HNF1 alpha appears in the liver kidney intestines and pancreas. It binds to enhancer elements of target genes affecting the transcriptional activity. The protein is also vital for cellular differentiation and maintenance in tissues where it is expressed.
Biological function summary: The HNF1 alpha functions as part of the dimeric HNF1 protein complex often partnering with HNF1 beta. It governs a wide array of genes including those involved in carbohydrate and lipid metabolism. By influencing the gene expression HNF1 alpha assists in maintaining normal metabolic processes. Its activity is critical for proper liver function and insulin secretion from pancreatic beta cells.
Pathways: HNF1 alpha actively integrates into key metabolic pathways such as the gluconeogenesis and the cholesterol biosynthesis pathways. In these pathways it works alongside other proteins like HNF4 alpha and PGC-1 alpha contributing significantly to glucose and lipid homeostasis. The transcription factor impacts the metabolic pathway reinforcing its integral role in maintaining energy balance and metabolic health.
Associated diseases and disorders: HNF1 alpha is importantly linked to maturity-onset diabetes of the young (MODY) and certain forms of liver disease. Mutations or dysregulation in HNF1 alpha can cause MODY3 a subtype of diabetes characterized by an autosomal dominant pattern of inheritance. Additionally the abnormal activity of HNF1 alpha may contribute to liver dysfunction often in conjunction with HNF4 alpha disruptions. These associations highlight the importance of HNF1 alpha in both metabolic pathway integrity and disease pathology.

Product promise

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11 product images

ChIC/CUT&RUN sequencing - Anti-HNF1 alpha antibody [RM1094] (ab316870)
ChIC/CUT&RUN sequencing - Anti-HNF1 alpha antibody [RM1094] (ab316870)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HepG2 (Human liver hepatocellular carcinoma cell line) cells and 5 µg of ab316870 [RM1094]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
ChIC/CUT&RUN sequencing - Anti-HNF1 alpha antibody [RM1094] (ab316870)
ChIC/CUT&RUN sequencing - Anti-HNF1 alpha antibody [RM1094] (ab316870)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HepG2 (Human liver hepatocellular carcinoma cell line) cells and 5 µg of ab316870 [RM1094]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
ChIC/CUT&RUN sequencing - Anti-HNF1 alpha antibody [RM1094] (ab316870)
ChIC/CUT&RUN sequencing - Anti-HNF1 alpha antibody [RM1094] (ab316870)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HepG2 (Human liver hepatocellular carcinoma cell line) cells and 5 µg of ab316870 [RM1094]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
Western blot - Anti-HNF1 alpha antibody [RM1094] (ab316870)
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-HNF1 alpha antibody [RM1094] (ab316870) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 81 kDa, 36 kDa
Exposure time: 37s
Western blot - Anti-HNF1 alpha antibody [RM1094] (ab316870)
Negative control: Hela.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
The molecular weight observed is consistent with what has been described in the literature (PMID: 31145732).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-HNF1 alpha antibody [RM1094] (ab316870) at 1/1000 dilution
Lane 1: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: Hela (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 35 kDa, 81 kDa, 36 kDa
Exposure time: 37s
Immunoprecipitation - Anti-HNF1 alpha antibody [RM1094] (ab316870)
HNF1 alpha was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab316870 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316870 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

Lane 2: ab316870 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab316870 in HepG2 whole cell lysate
Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.
All lanes: Immunoprecipitation - Anti-HNF1 alpha antibody [RM1094] (ab316870) at 1/30 dilution
All lanes: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 67s
Flow Cytometry (Intracellular) - Anti-HNF1 alpha antibody [RM1094] (ab316870)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell, Left) / HepG2 (human hepatocellular carcinoma epithelial cell, Right) cells labelling HNF1 alpha with ab316870 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control: HeLa.
Immunocytochemistry/ Immunofluorescence - Anti-HNF1 alpha antibody [RM1094] (ab316870)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling HNF1 alpha with ab316870 at 1/500 (1.04 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing nuclear staining in HepG2 cell line.

Negative control: HeLa.

Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [RM1094] (ab316870)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling HNF1 alpha with ab316870 at 1/4000 (0.129 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Negative control: no staining on human skeletal msucle. The section was incubated with ab316870 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [RM1094] (ab316870)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HNF1 alpha with ab316870 at 1/4000 (0.129 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human colon. The section was incubated with ab316870 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [RM1094] (ab316870)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling HNF1 alpha with ab316870 at 1/4000 (0.129 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human pancreas. The section was incubated with ab316870 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins