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AB240965

Anti-HNF6 antibody [EPR14976] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal HNF6 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

HNF6, HNF6A, ONECUT1, Hepatocyte nuclear factor 6, HNF-6, One cut domain family member 1, One cut homeobox 1

1 Images
Western blot - Anti-HNF6 antibody [EPR14976] - BSA and Azide free (AB240965)
  • WB

Supplier Data

Western blot - Anti-HNF6 antibody [EPR14976] - BSA and Azide free (AB240965)

All lanes:

Western blot - Anti-HNF6 antibody [EPR14976] - BSA and Azide free (ab240965) at 1/1000 dilution

Lane 1:

Human fetal liver cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14976

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab240965 is the carrier-free version of ab186743.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hepatocyte nuclear factor 6 (HNF6) also known as Onecut1 is a transcription factor that functions to regulate gene expression. It has a molecular mass of approximately 55 kDa. HNF6 is expressed in the liver pancreas and several other tissues influencing the development and function of these organs. It plays a significant role in regulating genes involved in various cellular processes by binding to DNA sequences and controlling transcription.
Biological function summary

This transcription factor controls the development and maturation of pancreatic islets and hepatocytes. It does not usually function as part of a larger protein complex but it directly influences genes that are vital for these processes. HNF6 ensures proper liver cell differentiation and has an important role in managing glucose metabolism and bile acid homeostasis in tissues where it is expressed.

Pathways

Hepatocyte nuclear factor 6 is integral to the glucose metabolism and bile acid biosynthesis pathways. It interacts with other transcription factors such as HNF4α and FOXA2 forming a regulatory network. HNF6 affects gene expression involved in maintaining metabolic balance and liver detoxification processes illustrating its critical influence on metabolic function within these pathways.

HNF6 links to diabetes and liver diseases. Its modulation of pancreatic development and function implicates it in both type 1 and type 2 diabetes often involving its interaction with other transcription factors like PDX1 and MafA. Similarly changes in HNF6 expression affect liver diseases such as cholestasis where it impacts bile acid regulation and liver cell function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.
See full target information ONECUT1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 21:2209-2219 PubMed32186759

2020

PDGFD induces ibrutinib resistance of diffuse large B‑cell lymphoma through activation of EGFR.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Jin,Leiping Wang,Zhonghua Tao,Jian Zhang,Fangfang Lv,Junning Cao,Xichun Hu
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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