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Mouse Monoclonal hnRNP A1 antibody. Suitable for IHC-P, IP, Flow Cyt, ELISA, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 58 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human HNRNPA1.


Images

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

Preservative: 0.1% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Native Full Length Protein corresponding to Human HNRNPA1. Database link P09651
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytELISAWBICC/IF
Human
Tested
Tested
Tested
Expected
Tested
Expected
Mouse
Predicted
Predicted
Predicted
Expected
Predicted
Expected

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

This antibody does not IP the hnRNP complex.

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1 µg for 106 Cells

Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

-

Notes

See Abreview (April 2, 2007).

Species

Human

Dilution info

-

Notes

See Abreview (April 2, 2007).

Target data

Function

Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection (PubMed:17371836). May bind to specific miRNA hairpins (PubMed:28431233). Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1 (PubMed:31498791).(Microbial infection) May play a role in HCV RNA replication.(Microbial infection) Cleavage by Enterovirus 71 protease 3C results in increased translation of apoptosis protease activating factor APAF1, leading to apoptosis.

Alternative names

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Mouse Monoclonal hnRNP A1 antibody. Suitable for IHC-P, IP, Flow Cyt, ELISA, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 58 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human HNRNPA1.

Alternative names

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Native Full Length Protein corresponding to Human HNRNPA1. Database link P09651
Clone number

9H10

Purification technique

Affinity purification Protein A

Concentration
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Purification notes

Purified from tissue culture supernatant.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

HnRNP A1 is involved in RNA binding and splicing contributing to the formation of spliceosomes the complexes responsible for pre-mRNA splicing. The protein aids in alternative splicing influencing mRNA diversity and stability. hnRNP A1 interacts with other proteins in the hnRNP family remodeling ribonucleoprotein complexes and affecting their functions. These interactions ensure proper mRNA maturation impacting gene expression regulation within the cell.

Activity summary

Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) also known just as hnRNP A1 is a multifunctional protein that plays important roles in RNA processing mechanisms. It is a member of the hnRNP protein family involved in the packaging of nascent pre-mRNA. hnRNP A1 has a molecular mass of approximately 34-38 kDa and shows a widespread expression in various cells and tissues. This protein is recognized for its ability to bind to RNA molecules contributing to their proper processing splicing and transport within the cellular context.

Pathways

HnRNP A1 is integral to critical cellular mechanisms such as the regulation of mRNA transport and splicing. It influences the DNA damage response pathway by modulating gene expression required for cellular repair and stability. hnRNP A1 also cooperates with other RNA binding proteins like the splicing factors SR proteins to orchestrate the splicing machinery. These interactions are pivotal in maintaining cellular homeostasis and response to stress.

Associated diseases and disorders

HnRNP A1 is notably related to neurodegenerative diseases like Amyotrophic Lateral Sclerosis (ALS) and certain types of cancer. Abnormalities in its expression or mutations can lead to disrupted cellular processes contributing to disease pathology. In ALS dysregulation of hnRNP A1 affects neuronal RNA metabolism and is associated with the TDP-43 protein a marker of neurodegeneration. In cancer hnRNP A1 influences tumor development through its role in alternative splicing and gene expression modification linking it to oncogenic signaling pathways.

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5 product images

  • Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832), expandable thumbnail

    Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832)

    All lanes: Western blot - Anti-hnRNP A1 antibody [9H10] (AB5832) at 1 µg/mL

    Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Lane 3: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 4: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (AB97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa

    Observed band size: 37 kDa, 43 kDa

    Exposure time: 30s

  • Flow Cytometry - Anti-hnRNP A1 antibody [9H10] (ab5832), expandable thumbnail

    Flow Cytometry - Anti-hnRNP A1 antibody [9H10] (ab5832)

    Overlay histogram showing Jurkat cells stained with ab5832 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5832, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [9H10] (ab5832), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [9H10] (ab5832)

    Ab5832 staining human lung. Staining is localized to the nucleus.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, citrate pH 6.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • Immunoprecipitation - Anti-hnRNP A1 antibody [9H10] (ab5832), expandable thumbnail

    Immunoprecipitation - Anti-hnRNP A1 antibody [9H10] (ab5832)

    hnRNP A1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to hnRNP A1 (ab5832) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab5832.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 37kDa: hnRNP A1; 42kDa:We are unsure as to the identity of this extra band.

    All lanes: Immunoprecipitation - Anti-hnRNP A1 antibody [9H10] (AB5832)

    Predicted band size: 38 kDa

  • Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832)

    Knockdown for 72 hours.

    All lanes: Western blot - Anti-hnRNP A1 antibody [9H10] (AB5832) at 1/1000 dilution

    All lanes: Mouse NSC34 whole cell lysate at 10 µg

    Secondary

    All lanes: IRDye® 700DX-conjugated Donkey anti-Mouse IgG at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 38 kDa

    Exposure time: 1min

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Product protocols

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