Anti-hnRNP A1 antibody [9H10] (ab5832) is a mouse monoclonal antibody detecting hnRNP A1 in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF, ELISA. Suitable for Human, Mouse.
- Over 50 publications
- Trusted since 2003
Images
![Western blot - Anti-hnRNP A1 antibody [9H10] (AB5832), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-antibody-9h10-ab5832--western-blot-img95144.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-hnRNP A1 antibody [9H10] (AB5832)")
![Flow Cytometry - Anti-hnRNP A1 antibody [9H10] (AB5832), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-antibody-9h10-ab5832--flow-cytometry-img122147.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-hnRNP A1 antibody [9H10] (AB5832)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [9H10] (AB5832), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-antibody-9h10-ab5832--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80434.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [9H10] (AB5832)")
![Immunoprecipitation - Anti-hnRNP A1 antibody [9H10] (AB5832), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-antibody-9h10-ab5832--immunoprecipitation-img9670.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-hnRNP A1 antibody [9H10] (AB5832)")
![Western blot - Anti-hnRNP A1 antibody [9H10] (AB5832), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-antibody-9h10-ab5832--western-blot-img138702.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-hnRNP A1 antibody [9H10] (AB5832)")
Publications
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- International journal of nanomedicine 18:5943-59602023M2 Macrophage-Derived Exosomal lncRNA MIR4435-2HG Promotes Progression of Infantile Hemangiomas by Targeting HNRNPA1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiyu Li,Zhongying Cao,Nanxi Li,Luying Wang,Cong Fu,Ran Huo,Guangqi Xu,Chonglin Tian,Jianhai BiPubMed 37881607
- Cell reports 41:1116752022Dynamic quality control machinery that operates across compartmental borders mediates the degradation of mammalian nuclear membrane proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPei-Ling Tsai,Christopher J F Cameron,Maria Fernanda Forni,Renee R Wasko,Brigitte S Naughton,Valerie Horsley,Mark B Gerstein,Christian SchliekerPubMed 36417855
- Journal of immunology research 2022:19175852022Asiaticoside Suppresses Gastric Cancer Progression and Induces Endoplasmic Reticulum Stress through the miR-635/HMGA1 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Zhang,Xiaolin Ji,Zhenguang Chen,Zhichao YaoPubMed 35692504
- The Journal of clinical investigation 132:2022KRAS mutant-driven SUMOylation controls extracellular vesicle transmission to trigger lymphangiogenesis in pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuming Luo,Zhihua Li,Yao Kong,Wang He,Hanhao Zheng,Mingjie An,Yan Lin,Dingwen Zhang,Jiabin Yang,Yue Zhao,Changhao Chen,Rufu ChenPubMed 35579947
- International journal of molecular sciences 23:2022p97/UBXD1 Generate Ubiquitylated Proteins That Are Sequestered into Nuclear Envelope Herniations in Torsin-Deficient Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah M Prophet,Brigitte S Naughton,Christian SchliekerPubMed 35563018
- Neurology. Genetics 7:e6322021Dominant Distal Myopathy 3 (MPD3) Caused by a Deletion in the Gene.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeter Hackman,Salla M Rusanen,Mridul Johari,Anna Vihola,Per Harald Jonson,Jaakko Sarparanta,Kati Donner,Päivi Lahermo,Sampo Koivunen,Helena Luque,Merja Soininen,Ibrahim Mahjneh,Mari Auranen,Meharji Arumilli,Marco Savarese,Bjarne UddPubMed 34722876
- International journal of molecular sciences 22:2021Identification of Abundant and Functional dodecaRNAs (doRNAs) Derived from Ribosomal RNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarine Lambert,Abderrahim Benmoussa,Idrissa Diallo,Katheryn Ouellet-Boutin,Véronique Dorval,Nathalie Majeau,Charles Joly-Beauparlant,Arnaud Droit,Alain Bergeron,Bernard Têtu,Yves Fradet,Frédéric Pouliot,Patrick ProvostPubMed 34575920
- eLife 10:2021PRMT5 regulates ovarian follicle development by facilitating translation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Chen,Fangfang Dong,Min Chen,Zhiming Shen,Haowei Wu,Changhuo Cen,Xiuhong Cui,Shilai Bao,Fei GaoPubMed 34448450
- Microbiology spectrum 9:e00364212021Prognostic Performance of Peripheral Blood Biomarkers in Identifying Seropositive Individuals at Risk of Developing Clinically Symptomatic Chagas Cardiomyopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSubhadip Choudhuri,Suresh K Bhavnani,Weibin Zhang,Valentina Botelli,Natalia Barrientos,Facundo Iñiguez,Maria Paola Zago,Nisha Jain GargPubMed 34479416
- The American journal of drug and alcohol abuse 47:581-5892021Differential expression and transcription factor binding associated with genotype at a pharmacogenetic variant in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesRichard C Crist,Gabriella Arauco-Shapiro,Alexander Zhang,Benjamin C Reiner,Wade H Berrettini,Glenn A DoylePubMed 34407719
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Native Full Length Protein corresponding to Human HNRNPA1. Database link P09651
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | ELISA | WB | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected | Tested | Expected |
| Mouse | Predicted | Predicted | Predicted | Expected | Predicted | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes This antibody does not IP the hnRNP complex. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes See Abreview (April 2, 2007). |
Species Human | Dilution info - | Notes See Abreview (April 2, 2007). |
Associated Products
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Target data
Function
Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and modulation of splice site selection (PubMed:17371836). Plays a role in the splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808). Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1 (PubMed:31498791). May bind to specific miRNA hairpins (PubMed:28431233). (Microbial infection) May play a role in HCV RNA replication. (Microbial infection) Cleavage by Enterovirus 71 protease 3C results in increased translation of apoptosis protease activating factor APAF1, leading to apoptosis.
Alternative names
HNRPA1, HNRNPA1, Heterogeneous nuclear ribonucleoprotein A1, hnRNP A1, Helix-destabilizing protein, Single-strand RNA-binding protein, hnRNP core protein A1
Recommended products
Anti-hnRNP A1 antibody [9H10] (ab5832) is a mouse monoclonal antibody detecting hnRNP A1 in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF, ELISA. Suitable for Human, Mouse.
- Over 50 publications
- Trusted since 2003
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Native Full Length Protein corresponding to Human HNRNPA1. Database link P09651
- Clone number
- 9H10
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Purified from tissue culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-hnRNP A1 antibody [9H10] (ab5832) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human, Mouse samples.
What is the molecular weight of hnRNP A1?
Anti-hnRNP A1 [9H10] (ab5832) specifically detects a band for hnRNP A1 (UniProt: P09651) at a molecular weight of 38kDa.
Trusted by the scientific community
Anti-hnRNP A1 [9H10] (ab5832) was first used in a scientific publication in 2003 and has been cited over 50 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) also known just as hnRNP A1 is a multifunctional protein that plays important roles in RNA processing mechanisms. It is a member of the hnRNP protein family involved in the packaging of nascent pre-mRNA. hnRNP A1 has a molecular mass of approximately 34-38 kDa and shows a widespread expression in various cells and tissues. This protein is recognized for its ability to bind to RNA molecules contributing to their proper processing splicing and transport within the cellular context.
- Biological function summary
HnRNP A1 is involved in RNA binding and splicing contributing to the formation of spliceosomes the complexes responsible for pre-mRNA splicing. The protein aids in alternative splicing influencing mRNA diversity and stability. hnRNP A1 interacts with other proteins in the hnRNP family remodeling ribonucleoprotein complexes and affecting their functions. These interactions ensure proper mRNA maturation impacting gene expression regulation within the cell.
- Pathways
HnRNP A1 is integral to critical cellular mechanisms such as the regulation of mRNA transport and splicing. It influences the DNA damage response pathway by modulating gene expression required for cellular repair and stability. hnRNP A1 also cooperates with other RNA binding proteins like the splicing factors SR proteins to orchestrate the splicing machinery. These interactions are pivotal in maintaining cellular homeostasis and response to stress.
- Associated diseases and disorders
HnRNP A1 is notably related to neurodegenerative diseases like Amyotrophic Lateral Sclerosis (ALS) and certain types of cancer. Abnormalities in its expression or mutations can lead to disrupted cellular processes contributing to disease pathology. In ALS dysregulation of hnRNP A1 affects neuronal RNA metabolism and is associated with the TDP-43 protein a marker of neurodegeneration. In cancer hnRNP A1 influences tumor development through its role in alternative splicing and gene expression modification linking it to oncogenic signaling pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
![Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-antibody-9h10-ab5832--western-blot-img95144.jpg "Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832)")
Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832)
All lanes: Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 3: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 37 kDa, 43 kDa
Exposure time: 30s
![Flow Cytometry - Anti-hnRNP A1 antibody [9H10] (ab5832), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-antibody-9h10-ab5832--flow-cytometry-img122147.jpg "Flow Cytometry - Anti-hnRNP A1 antibody [9H10] (ab5832)")
Flow Cytometry - Anti-hnRNP A1 antibody [9H10] (ab5832)
Overlay histogram showing Jurkat cells stained with ab5832 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5832, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (Mouse IgG2b [PLPV219] - Isotype Control ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [9H10] (ab5832), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-antibody-9h10-ab5832--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80434.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [9H10] (ab5832)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [9H10] (ab5832)
ab5832 staining human lung. Staining is localized to the nucleus.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, citrate pH 6.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.![Immunoprecipitation - Anti-hnRNP A1 antibody [9H10] (ab5832), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-antibody-9h10-ab5832--immunoprecipitation-img9670.jpg "Immunoprecipitation - Anti-hnRNP A1 antibody [9H10] (ab5832)")
Immunoprecipitation - Anti-hnRNP A1 antibody [9H10] (ab5832)
hnRNP A1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to hnRNP A1 (ab5832) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab5832.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 37kDa: hnRNP A1; 42kDa:We are unsure as to the identity of this extra band.All lanes: Immunoprecipitation - Anti-hnRNP A1 antibody [9H10] (ab5832)
Predicted band size: 38 kDa
![Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-antibody-9h10-ab5832--western-blot-img138702.jpg "Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832)")
This image is courtesy of an anonymous customer review.Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832)
Knockdown for 72 hours.
All lanes: Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832) at 1/1000 dilution
Lane 1: Mouse NSC34 whole cell lysate at 10 µg with control siRNA
Lane 2: Mouse NSC34 whole cell lysate at 10 µg with hnRNP A1 siRNA
SecondaryAll lanes: IRDye® 700DX-conjugated Donkey anti-Mouse IgG at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 38 kDa
Exposure time: 1min
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