AB137780

Anti-hnRNP A1 antibody

Name: Anti-hnRNP A1 antibody (ab137780) | Abcam
Brand: Abcam Limited
SKU: AB137780
Price: 545 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal hnRNP A1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HNRNPA1 aa 1-250.

View Alternative Names

HNRPA1, HNRNPA1, Heterogeneous nuclear ribonucleoprotein A1, hnRNP A1, Helix-destabilizing protein, Single-strand RNA-binding protein, hnRNP core protein A1

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody (AB137780)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody (AB137780)

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labeling hnRNP A1 with ab137780 at a 1/200 dilution. The lower image (labeled '2') was merged with DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody (AB137780)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody (AB137780)

Immunohistochemical analysis of paraffin-embedded mouse heart tissue stained for hnRNP A1 protein at nucleus with ab137780 at a 1/500 dilution.

Antigen retrieval : EDTA based buffer, (pH 8.0), 15min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody (AB137780)

Immunohistochemical analysis of paraffin-embedded mouse heart tissue stained for hnRNP A1 protein at nucleus with ab137780 at a 1/500 dilution.

Antigen retrieval : EDTA based buffer, (pH 8.0), 15min

Lab

Western blot - Anti-hnRNP A1 antibody (AB137780)

All lanes:

Western blot - Anti-hnRNP A1 antibody (ab137780) at 1/3000 dilution

Lane 1:

WT 293T cell extracts at 30 µg

Lane 2:

hnRNP A1 Knockout 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 38 kDa

false

Unknown

Western blot - Anti-hnRNP A1 antibody (AB137780)

12% SDS PAGE

All lanes:

Western blot - Anti-hnRNP A1 antibody (ab137780) at 1/500 dilution

Lane 1:

H1299 whole cell lysate at 30 µg

Lane 2:

HelaS3 whole cell lysate at 30 µg

Predicted band size: 38 kDa

false

Supplier Data

Western blot - Anti-hnRNP A1 antibody (AB137780)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-hnRNP A1 antibody (ab137780) at 1/1000 dilution

All lanes:

PC-12 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 39 kDa

false

Unknown

Western blot - Anti-hnRNP A1 antibody (AB137780)

10% SDS PAGE

All lanes:

Western blot - Anti-hnRNP A1 antibody (ab137780) at 1/1000 dilution

All lanes:

Mouse brain cell lysate at 50 µg

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human HNRNPA1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P09651

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) also known just as hnRNP A1 is a multifunctional protein that plays important roles in RNA processing mechanisms. It is a member of the hnRNP protein family involved in the packaging of nascent pre-mRNA. hnRNP A1 has a molecular mass of approximately 34-38 kDa and shows a widespread expression in various cells and tissues. This protein is recognized for its ability to bind to RNA molecules contributing to their proper processing splicing and transport within the cellular context.

Biological function summary

HnRNP A1 is involved in RNA binding and splicing contributing to the formation of spliceosomes the complexes responsible for pre-mRNA splicing. The protein aids in alternative splicing influencing mRNA diversity and stability. hnRNP A1 interacts with other proteins in the hnRNP family remodeling ribonucleoprotein complexes and affecting their functions. These interactions ensure proper mRNA maturation impacting gene expression regulation within the cell.

Pathways

HnRNP A1 is integral to critical cellular mechanisms such as the regulation of mRNA transport and splicing. It influences the DNA damage response pathway by modulating gene expression required for cellular repair and stability. hnRNP A1 also cooperates with other RNA binding proteins like the splicing factors SR proteins to orchestrate the splicing machinery. These interactions are pivotal in maintaining cellular homeostasis and response to stress.

HnRNP A1 is notably related to neurodegenerative diseases like Amyotrophic Lateral Sclerosis (ALS) and certain types of cancer. Abnormalities in its expression or mutations can lead to disrupted cellular processes contributing to disease pathology. In ALS dysregulation of hnRNP A1 affects neuronal RNA metabolism and is associated with the TDP-43 protein a marker of neurodegeneration. In cancer hnRNP A1 influences tumor development through its role in alternative splicing and gene expression modification linking it to oncogenic signaling pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and modulation of splice site selection (PubMed : 17371836). Plays a role in the splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808). Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1 (PubMed : 31498791). May bind to specific miRNA hairpins (PubMed : 28431233).. (Microbial infection) May play a role in HCV RNA replication.. (Microbial infection) Cleavage by Enterovirus 71 protease 3C results in increased translation of apoptosis protease activating factor APAF1, leading to apoptosis.

See full target information HNRNPA1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10925 PubMed39738171

2024

NS1 binding protein regulates stress granule dynamics and clearance by inhibiting p62 ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Pureum Jeon,Hyun-Ji Ham,Haneul Choi,Semin Park,Jae-Woo Jang,Sang-Won Park,Dong-Hyung Cho,Hyun-Jeong Lee,Hyun Kyu Song,Masaaki Komatsu,Dohyun Han,Deok-Jin Jang,Jin-A Lee

Kidney international 95:1373-1388 PubMed30979564

2019

Mechanisms of GLP-1 receptor-independent renoprotective effects of the dipeptidyl peptidase type 4 inhibitor linagliptin in GLP-1 receptor knockout mice with 5/6 nephrectomy.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed A Hasan,Karoline von Websky,Christoph Reichetzeder,Oleg Tsuprykov,Mohamed M S Gaballa,Jingli Guo,Shufei Zeng,Denis Delić,Harald Tammen,Thomas Klein,Burkhard Kleuser,Berthold Hocher

BioMedicine 5:10 PubMed26048696

2015

P-coumaric acid regulates exon 12 splicing of the ATP7B gene by modulating hnRNP A1 protein expressions.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Ju Lin,Tsung-Jung Ho,Ting-Hsu Lin,Wei-Yi Hsu,Shao-Mei Huang,Chiu-Chu Liao,Chih-Ho Lai,Xiang Liu,Hsinyi Tsang,Chien-Chen Lai,Fuu-Jen Tsai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-hnRNP A1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody (AB137780)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody (AB137780)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody (AB137780)

Western blot - Anti-hnRNP A1 antibody (AB137780)

Western blot - Anti-hnRNP A1 antibody (AB137780)

Western blot - Anti-hnRNP A1 antibody (AB137780)

Western blot - Anti-hnRNP A1 antibody (AB137780)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

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Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise