Rabbit Recombinant Monoclonal hnRNP A1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 12 publications.
Images
![Western blot - Anti-hnRNP A1 antibody [EPR12768] (AB177152), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-antibody-epr12768-ab177152--western-blot-img136285.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-hnRNP A1 antibody [EPR12768] (AB177152)")
![Western blot - Anti-hnRNP A1 antibody [EPR12768] (AB177152), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-antibody-epr12768-ab177152--western-blot-img102810.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-hnRNP A1 antibody [EPR12768] (AB177152)")
Publications
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- Heliyon 9:e211702023AGEs induce high expression of Dll4 via endoplasmic reticulum stress PERK signaling-mediated internal ribosomal entry site mechanism in macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanpeng Ma,Shixiang Zheng,Xiqiang Wang,Ling Zhu,Junkui Wang,Shuo Pan,Yong Zhang,Zhongwei LiuPubMed 37886757
- Frontiers in pharmacology 12:7918892022LncRNA LINC01134 Contributes to Radioresistance in Hepatocellular Carcinoma by Regulating DNA Damage Response MAPK Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiyi Wang,Xinxing Wang,Zhonghou Rong,Longfei Dai,Chengkun Qin,Shikang Wang,Wenmao GengPubMed 35173610
- Scientific reports 11:244442021Identification of an mRNA isoform switch for HNRNPA1 in breast cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMurat Erdem,İbrahim Ozgul,Didem Naz Dioken,Irmak Gurcuoglu,Sezen Guntekin Ergun,Rengul Cetin-Atalay,Tolga Can,Ayse Elif Erson-BensanPubMed 34961772
- Journal of translational medicine 19:4922021CircSERPINA3 regulates SERPINA3-mediated apoptosis, autophagy and aerobic glycolysis of prostate cancer cells by competitively binding to MiR-653-5p and recruiting BUD13.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZengshu Xing,Sailian Li,Zhenxiang Liu,Chong Zhang,Zhiming BaiPubMed 34861864
- Cell death & disease 12:2552021HNRNPA1-mediated exosomal sorting of miR-483-5p out of renal tubular epithelial cells promotes the progression of diabetic nephropathy-induced renal interstitial fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongWei Liu,FengXun Liu,ZhengYong Li,ShaoKang Pan,JunWei Xie,ZiHao Zhao,ZhenJie Liu,JiaHui Zhang,ZhangSuo LiuPubMed 33692334
- Scientific reports 10:221552020Identification of hnRNP-A1 as a pharmacodynamic biomarker of type I PRMT inhibition in blood and tumor tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaul B Noto et. al.PubMed 33335114
- Neuropathology : official journal of the Japanese Society of Neuropathology 39:358-3672019Expanded polyglutamine impairs normal nuclear distribution of fused in sarcoma and poly (rC)-binding protein 1 in Huntington's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShinichiro Mori,Hiroyuki Honda,Takashi Ishii,Motoi Yoshimura,Naokazu Sasagasako,Satoshi O Suzuki,Takayuki Taniwaki,Toru IwakiPubMed 31599037
- Aging 11:4407-44372019HNRNPA1-mediated 3' UTR length changes of contributes to cancer- and senescence-associated phenotypes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Jia,Hongbo Nie,Peng Yu,Baiyun Xie,Chenji Wang,Fu Yang,Gang Wei,Ting NiPubMed 31257225
- International journal of biological macromolecules 129:140-1512019Prognostic value and oncogene function of heterogeneous nuclear ribonucleoprotein A1 overexpression in HBV-related hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui-Sheng Ke et. al.PubMed 30731163
- Oncology letters 16:3746-37562018Prognostic value of heterogeneous ribonucleoprotein A1 expression and inflammatory indicators for patients with surgically resected hepatocellular carcinoma: Perspectives from a high occurrence area of hepatocellular carcinoma in China.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuisheng Ke,Lizhi Lv,Jiayan Li,Xiaojin Zhang,Fang Yang,Kun Zhang,Yi JiangPubMed 30127985
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested | Tested |
| Mouse | Predicted | Not recommended | Predicted | Predicted | Predicted |
| Rat | Predicted | Not recommended | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
Target data
Function
Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and modulation of splice site selection (PubMed:17371836). Plays a role in the splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808). Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1 (PubMed:31498791). May bind to specific miRNA hairpins (PubMed:28431233). (Microbial infection) May play a role in HCV RNA replication. (Microbial infection) Cleavage by Enterovirus 71 protease 3C results in increased translation of apoptosis protease activating factor APAF1, leading to apoptosis.
Alternative names
HNRPA1, HNRNPA1, Heterogeneous nuclear ribonucleoprotein A1, hnRNP A1, Helix-destabilizing protein, Single-strand RNA-binding protein, hnRNP core protein A1
Recommended products
Rabbit Recombinant Monoclonal hnRNP A1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 12 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12768
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) also known just as hnRNP A1 is a multifunctional protein that plays important roles in RNA processing mechanisms. It is a member of the hnRNP protein family involved in the packaging of nascent pre-mRNA. hnRNP A1 has a molecular mass of approximately 34-38 kDa and shows a widespread expression in various cells and tissues. This protein is recognized for its ability to bind to RNA molecules contributing to their proper processing splicing and transport within the cellular context.
- Biological function summary
HnRNP A1 is involved in RNA binding and splicing contributing to the formation of spliceosomes the complexes responsible for pre-mRNA splicing. The protein aids in alternative splicing influencing mRNA diversity and stability. hnRNP A1 interacts with other proteins in the hnRNP family remodeling ribonucleoprotein complexes and affecting their functions. These interactions ensure proper mRNA maturation impacting gene expression regulation within the cell.
- Pathways
HnRNP A1 is integral to critical cellular mechanisms such as the regulation of mRNA transport and splicing. It influences the DNA damage response pathway by modulating gene expression required for cellular repair and stability. hnRNP A1 also cooperates with other RNA binding proteins like the splicing factors SR proteins to orchestrate the splicing machinery. These interactions are pivotal in maintaining cellular homeostasis and response to stress.
- Associated diseases and disorders
HnRNP A1 is notably related to neurodegenerative diseases like Amyotrophic Lateral Sclerosis (ALS) and certain types of cancer. Abnormalities in its expression or mutations can lead to disrupted cellular processes contributing to disease pathology. In ALS dysregulation of hnRNP A1 affects neuronal RNA metabolism and is associated with the TDP-43 protein a marker of neurodegeneration. In cancer hnRNP A1 influences tumor development through its role in alternative splicing and gene expression modification linking it to oncogenic signaling pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-antibody-epr12768-ab177152--western-blot-img136285.jpg "Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152)")
Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
Lanes 1-3: Merged signal (red and green). Green - ab177152 observed at 37 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.ab177152 Anti-hnRNP A1 antibody [EPR12768] was shown to specifically react with hnRNP A1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human HNRNPA1 knockout HEK-293T cell line ab266193 (knockout cell lysate Human HNRNPA1 knockout HEK-293T cell lysate ab256942) was used. Wild-type and hnRNP A1 knockout samples were subjected to SDS-PAGE. ab177152 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152) at 1/1000 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: HNRNPA1 knockout HEK293T cell lysate at 20 µg
Lane 2: Western blot - Human HNRNPA1 knockout HEK-293T cell line (Human HNRNPA1 knockout HEK-293T cell line ab266193)
Lane 3: HeLa cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 23 kDa, 25 kDa, 33 kDa, 38 kDa, 53 kDa, 72 kDa
Observed band size: 25 kDa, 27 kDa, 37 kDa, 38 kDa, 58 kDa, 72 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-antibody-epr12768-ab177152--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136743.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling hnRNP A1 with ab177152 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-antibody-epr12768-ab177152--western-blot-img102810.jpg "Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152)")
Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
All lanes: Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152) at 1/1000 dilution
Lane 1: MCF7 cell lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: K562 cell lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
Lane 5: Jurkat cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 38 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody [EPR12768] (ab177152), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-antibody-epr12768-ab177152--immunocytochemistry-immunofluorescence-img1413.gif "Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody [EPR12768] (ab177152)")
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
Immunofluorescent analysis of HepG2 cells labeling hnRNP A1 with ab177152 at 1/100 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-antibody-epr12768-ab177152--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img64451.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma tissue labeling hnRNP A1 with ab177152 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-hnRNP A1 antibody [EPR12768] (ab177152), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-antibody-epr12768-ab177152--flow-cytometry-intracellular-img50580.jpg "Flow Cytometry (Intracellular) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)")
Flow Cytometry (Intracellular) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
Intracellular flow cytometric analysis of permeabilized Jurkatcells labeling hnRNP A1with ab177152 at 1/10 dilution (red), or a rabbit IgG (negative) (green).
![Flow Cytometry (Intracellular) - Anti-hnRNP A1 antibody [EPR12768] (ab177152), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-antibody-epr12768-ab177152--flow-cytometry-intracellular-img50580.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)")
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