AB177152

Anti-hnRNP A1 antibody [EPR12768]

RabMAb
Recombinant
KO Validated
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(14 Publications)

Rabbit Recombinant Monoclonal hnRNP A1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 14 publications.

View Alternative Names

HNRPA1, HNRNPA1, Heterogeneous nuclear ribonucleoprotein A1, hnRNP A1, Helix-destabilizing protein, Single-strand RNA-binding protein, hnRNP core protein A1

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody [EPR12768] (AB177152)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody [EPR12768] (AB177152)

Immunofluorescent analysis of HepG2 cells labeling hnRNP A1 with ab177152 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (AB177152)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (AB177152)

Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma tissue labeling hnRNP A1 with ab177152 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-hnRNP A1 antibody [EPR12768] (AB177152)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-hnRNP A1 antibody [EPR12768] (AB177152)

Intracellular flow cytometric analysis of permeabilized Jurkatcells labeling hnRNP A1with ab177152 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (AB177152)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling hnRNP A1 with ab177152 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Lab

Western blot - Anti-hnRNP A1 antibody [EPR12768] (AB177152)

Lanes 1-3 : Merged signal (red and green). Green - ab177152 observed at 37 kDa. Red - loading control ab8245 observed at 36 kDa.

ab177152 Anti-hnRNP A1 antibody [EPR12768] was shown to specifically react with hnRNP A1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266193 (knockout cell lysate ab256942) was used. Wild-type and hnRNP A1 knockout samples were subjected to SDS-PAGE. ab177152 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

HNRNPA1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human HNRNPA1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-hnrnpa1-knockout-hek-293t-cell-line-ab266193'>ab266193</a>)

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 23 kDa,25 kDa,33 kDa,38 kDa,53 kDa,72 kDa

Observed band size: 25 kDa,27 kDa,37 kDa,38 kDa,58 kDa,72 kDa

false

Supplier Data

Western blot - Anti-hnRNP A1 antibody [EPR12768] (AB177152)

All lanes:

Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Lane 5:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 38 kDa

false

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-hnRNP A1 antibody [EPR12768]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-hnRNP A1 antibody [EPR12768]
Carrier free

Anti-hnRNP A1 antibody [EPR12768] - BSA and Azide free
660 APC

APC Anti-hnRNP A1 antibody [EPR12768]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-hnRNP A1 antibody [EPR12768]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-hnRNP A1 antibody [EPR12768]
578 PE

PE Anti-hnRNP A1 antibody [EPR12768]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-hnRNP A1 antibody [EPR12768]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12768

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) also known just as hnRNP A1 is a multifunctional protein that plays important roles in RNA processing mechanisms. It is a member of the hnRNP protein family involved in the packaging of nascent pre-mRNA. hnRNP A1 has a molecular mass of approximately 34-38 kDa and shows a widespread expression in various cells and tissues. This protein is recognized for its ability to bind to RNA molecules contributing to their proper processing splicing and transport within the cellular context.

Biological function summary

HnRNP A1 is involved in RNA binding and splicing contributing to the formation of spliceosomes the complexes responsible for pre-mRNA splicing. The protein aids in alternative splicing influencing mRNA diversity and stability. hnRNP A1 interacts with other proteins in the hnRNP family remodeling ribonucleoprotein complexes and affecting their functions. These interactions ensure proper mRNA maturation impacting gene expression regulation within the cell.

Pathways

HnRNP A1 is integral to critical cellular mechanisms such as the regulation of mRNA transport and splicing. It influences the DNA damage response pathway by modulating gene expression required for cellular repair and stability. hnRNP A1 also cooperates with other RNA binding proteins like the splicing factors SR proteins to orchestrate the splicing machinery. These interactions are pivotal in maintaining cellular homeostasis and response to stress.

HnRNP A1 is notably related to neurodegenerative diseases like Amyotrophic Lateral Sclerosis (ALS) and certain types of cancer. Abnormalities in its expression or mutations can lead to disrupted cellular processes contributing to disease pathology. In ALS dysregulation of hnRNP A1 affects neuronal RNA metabolism and is associated with the TDP-43 protein a marker of neurodegeneration. In cancer hnRNP A1 influences tumor development through its role in alternative splicing and gene expression modification linking it to oncogenic signaling pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and modulation of splice site selection (PubMed : 17371836). Plays a role in the splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808). Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1 (PubMed : 31498791). May bind to specific miRNA hairpins (PubMed : 28431233).. (Microbial infection) May play a role in HCV RNA replication.. (Microbial infection) Cleavage by Enterovirus 71 protease 3C results in increased translation of apoptosis protease activating factor APAF1, leading to apoptosis.

See full target information HNRNPA1

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109603 PubMed38638569

2024

RNA molecules display distinctive organization at nuclear speckles.

Applications

Unspecified application

Species

Unspecified reactive species

Sneha Paul,Mauricio A Arias,Li Wen,Susan E Liao,Jiacheng Zhang,Xiaoshu Wang,Oded Regev,Jingyi Fei

Heliyon 9:e21170 PubMed37886757

2023

AGEs induce high expression of Dll4 via endoplasmic reticulum stress PERK signaling-mediated internal ribosomal entry site mechanism in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Yanpeng Ma,Shixiang Zheng,Xiqiang Wang,Ling Zhu,Junkui Wang,Shuo Pan,Yong Zhang,Zhongwei Liu

Frontiers in pharmacology 12:791889 PubMed35173610

2022

LncRNA LINC01134 Contributes to Radioresistance in Hepatocellular Carcinoma by Regulating DNA Damage Response MAPK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyi Wang,Xinxing Wang,Zhonghou Rong,Longfei Dai,Chengkun Qin,Shikang Wang,Wenmao Geng

Scientific reports 11:24444 PubMed34961772

2021

Identification of an mRNA isoform switch for HNRNPA1 in breast cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Murat Erdem,İbrahim Ozgul,Didem Naz Dioken,Irmak Gurcuoglu,Sezen Guntekin Ergun,Rengul Cetin-Atalay,Tolga Can,Ayse Elif Erson-Bensan

Virulence 12:3125-3136 PubMed34923914

2021

Seneca Valley virus 3C degrades heterogeneous nuclear ribonucleoprotein A1 to facilitate viral replication.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangwei Song,Dan Wang,Rong Quan,Jue Liu

Journal of translational medicine 19:492 PubMed34861864

2021

CircSERPINA3 regulates SERPINA3-mediated apoptosis, autophagy and aerobic glycolysis of prostate cancer cells by competitively binding to MiR-653-5p and recruiting BUD13.

Applications

Unspecified application

Species

Unspecified reactive species

Zengshu Xing,Sailian Li,Zhenxiang Liu,Chong Zhang,Zhiming Bai

Cell death & disease 12:255 PubMed33692334

2021

HNRNPA1-mediated exosomal sorting of miR-483-5p out of renal tubular epithelial cells promotes the progression of diabetic nephropathy-induced renal interstitial fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

DongWei Liu,FengXun Liu,ZhengYong Li,ShaoKang Pan,JunWei Xie,ZiHao Zhao,ZhenJie Liu,JiaHui Zhang,ZhangSuo Liu

Scientific reports 10:22155 PubMed33335114

2020

Identification of hnRNP-A1 as a pharmacodynamic biomarker of type I PRMT inhibition in blood and tumor tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Paul B Noto,Timothy W Sikorski,Francesca Zappacosta,Craig D Wagner,Rocio Montes de Oca,Matthew E Szapacs,Roland S Annan,Yan Liu,Charles F McHugh,Helai P Mohammad,Steven P Piccoli,Caretha L Creasy

Neuropathology : official journal of the Japanese Society of Neuropathology 39:358-367 PubMed31599037

2019

Expanded polyglutamine impairs normal nuclear distribution of fused in sarcoma and poly (rC)-binding protein 1 in Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Shinichiro Mori,Hiroyuki Honda,Takashi Ishii,Motoi Yoshimura,Naokazu Sasagasako,Satoshi O Suzuki,Takayuki Taniwaki,Toru Iwaki

Aging 11:4407-4437 PubMed31257225

2019

HNRNPA1-mediated 3' UTR length changes of contributes to cancer- and senescence-associated phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Jia,Hongbo Nie,Peng Yu,Baiyun Xie,Chenji Wang,Fu Yang,Gang Wei,Ting Ni

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-hnRNP A1 antibody [EPR12768]

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody [EPR12768] (AB177152)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (AB177152)

Flow Cytometry (Intracellular) - Anti-hnRNP A1 antibody [EPR12768] (AB177152)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (AB177152)

Western blot - Anti-hnRNP A1 antibody [EPR12768] (AB177152)

Western blot - Anti-hnRNP A1 antibody [EPR12768] (AB177152)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise