Rabbit Recombinant Monoclonal hnRNP A1 citrulline R122 antibody. Suitable for IP, Dot, WB and reacts with Human, Synthetic peptide, Transfected cell lysate - Human samples.
Images
![Western blot - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] (AB208029), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-citrulline-r122-antibody-epr20177-ab208029--western-blot-img21227.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] (AB208029)")
![Immunoprecipitation - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] (AB208029), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-citrulline-r122-antibody-epr20177-ab208029--immunoprecipitation-img756.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] (AB208029)")
![Dot Blot - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] (AB208029), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-citrulline-r122-antibody-epr20177-ab208029--dot-blot-img4435.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] (AB208029)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Dot | WB | |
|---|---|---|---|
| Human | Tested | Expected | Expected |
| Synthetic peptide | Not recommended | Tested | Not recommended |
| Transfected cell lysate - Human | Not recommended | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/40 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide, Transfected cell lysate - Human | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and modulation of splice site selection (PubMed:17371836). Plays a role in the splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808). Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1 (PubMed:31498791). May bind to specific miRNA hairpins (PubMed:28431233). (Microbial infection) May play a role in HCV RNA replication. (Microbial infection) Cleavage by Enterovirus 71 protease 3C results in increased translation of apoptosis protease activating factor APAF1, leading to apoptosis.
Alternative names
HNRPA1, HNRNPA1, Heterogeneous nuclear ribonucleoprotein A1, hnRNP A1, Helix-destabilizing protein, Single-strand RNA-binding protein, hnRNP core protein A1
Recommended products
Rabbit Recombinant Monoclonal hnRNP A1 citrulline R122 antibody. Suitable for IP, Dot, WB and reacts with Human, Synthetic peptide, Transfected cell lysate - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR20177
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) also known just as hnRNP A1 is a multifunctional protein that plays important roles in RNA processing mechanisms. It is a member of the hnRNP protein family involved in the packaging of nascent pre-mRNA. hnRNP A1 has a molecular mass of approximately 34-38 kDa and shows a widespread expression in various cells and tissues. This protein is recognized for its ability to bind to RNA molecules contributing to their proper processing splicing and transport within the cellular context.
- Biological function summary
HnRNP A1 is involved in RNA binding and splicing contributing to the formation of spliceosomes the complexes responsible for pre-mRNA splicing. The protein aids in alternative splicing influencing mRNA diversity and stability. hnRNP A1 interacts with other proteins in the hnRNP family remodeling ribonucleoprotein complexes and affecting their functions. These interactions ensure proper mRNA maturation impacting gene expression regulation within the cell.
- Pathways
HnRNP A1 is integral to critical cellular mechanisms such as the regulation of mRNA transport and splicing. It influences the DNA damage response pathway by modulating gene expression required for cellular repair and stability. hnRNP A1 also cooperates with other RNA binding proteins like the splicing factors SR proteins to orchestrate the splicing machinery. These interactions are pivotal in maintaining cellular homeostasis and response to stress.
- Associated diseases and disorders
HnRNP A1 is notably related to neurodegenerative diseases like Amyotrophic Lateral Sclerosis (ALS) and certain types of cancer. Abnormalities in its expression or mutations can lead to disrupted cellular processes contributing to disease pathology. In ALS dysregulation of hnRNP A1 affects neuronal RNA metabolism and is associated with the TDP-43 protein a marker of neurodegeneration. In cancer hnRNP A1 influences tumor development through its role in alternative splicing and gene expression modification linking it to oncogenic signaling pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] (ab208029), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-citrulline-r122-antibody-epr20177-ab208029--western-blot-img21227.jpg "Western blot - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] (ab208029)")
Western blot - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] (ab208029)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] (ab208029) at 1/2000 dilution
Lanes 1 and 3: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a control vector containing GFP tag, treated with 10 mM calcium chloride and 10 at 20 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI2 (WT) expression vector, treated with 10 mM calcium chloride and at 20 µg
Lane 4: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 38 kDa
Exposure time: 8s
![Immunoprecipitation - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] (ab208029), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-citrulline-r122-antibody-epr20177-ab208029--immunoprecipitation-img756.jpg "Immunoprecipitation - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] (ab208029)")
Immunoprecipitation - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] (ab208029)
hnRNP A1 (citrulline R122) was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 μM Ionomycin for 2 hours whole cell lysate with ab208029 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab208029 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: HEK-293T transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 μM Ionomycin for 2 hours whole cell lysate 10 μg (Input).
Lane 2: ab208029 IP in HEK-293T transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 μM Ionomycin for 2 hours whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab208029 in HEK-293T transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 μM Ionomycin for 2 hours whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
All lanes: Immunoprecipitation - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] (ab208029)
Predicted band size: 38 kDa
![Dot Blot - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] (ab208029), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-citrulline-r122-antibody-epr20177-ab208029--dot-blot-img4435.jpg "Dot Blot - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] (ab208029)")
Dot Blot - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] (ab208029)
Dot blot analysis of hnRNP A1 (citrulline R122) labeled with ab208029 at 1/1000 dilution.
Lane 1: hnRNP A1 (citrulline R122) peptide;
Lane 2: hnRNP A1 non-citrulline peptide;
Lane 3: hnRNP A2B1 (citrulline R129) peptide.
Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Based on sequence homology this antibody cross reacts with hnRNP A2B1 (citrulline R129) and hnRNP A1L2 (citrulline R122).
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