Rabbit Recombinant Monoclonal hnRNP A1 citrulline R140 antibody. Carrier free. Suitable for Dot, WB and reacts with Recombinant full length protein, Recombinant fragment - Human samples.
Images
![Dot Blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (AB251488), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-citrulline-r140-antibody-epr20178-bsa-and-azide-free-ab251488--dot-blot-img1495.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (AB251488)")
![Western blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (AB251488), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-citrulline-r140-antibody-epr20178-bsa-and-azide-free-ab251488--western-blot-img12236.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (AB251488)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | |
|---|---|---|
| Recombinant fragment - Human | Not recommended | Tested |
| Recombinant full length protein | Tested | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
Target data
Function
Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and modulation of splice site selection (PubMed:17371836). Plays a role in the splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808). Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1 (PubMed:31498791). May bind to specific miRNA hairpins (PubMed:28431233). (Microbial infection) May play a role in HCV RNA replication. (Microbial infection) Cleavage by Enterovirus 71 protease 3C results in increased translation of apoptosis protease activating factor APAF1, leading to apoptosis.
Alternative names
HNRPA1, HNRNPA1, Heterogeneous nuclear ribonucleoprotein A1, hnRNP A1, Helix-destabilizing protein, Single-strand RNA-binding protein, hnRNP core protein A1
Recommended products
Rabbit Recombinant Monoclonal hnRNP A1 citrulline R140 antibody. Carrier free. Suitable for Dot, WB and reacts with Recombinant full length protein, Recombinant fragment - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20178
- Specificity
Based on sequence homology, we predict that this antibody may react with HNRNPA1L2.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251488 is the carrier-free version of Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] ab208030.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) also known just as hnRNP A1 is a multifunctional protein that plays important roles in RNA processing mechanisms. It is a member of the hnRNP protein family involved in the packaging of nascent pre-mRNA. hnRNP A1 has a molecular mass of approximately 34-38 kDa and shows a widespread expression in various cells and tissues. This protein is recognized for its ability to bind to RNA molecules contributing to their proper processing splicing and transport within the cellular context.
- Biological function summary
HnRNP A1 is involved in RNA binding and splicing contributing to the formation of spliceosomes the complexes responsible for pre-mRNA splicing. The protein aids in alternative splicing influencing mRNA diversity and stability. hnRNP A1 interacts with other proteins in the hnRNP family remodeling ribonucleoprotein complexes and affecting their functions. These interactions ensure proper mRNA maturation impacting gene expression regulation within the cell.
- Pathways
HnRNP A1 is integral to critical cellular mechanisms such as the regulation of mRNA transport and splicing. It influences the DNA damage response pathway by modulating gene expression required for cellular repair and stability. hnRNP A1 also cooperates with other RNA binding proteins like the splicing factors SR proteins to orchestrate the splicing machinery. These interactions are pivotal in maintaining cellular homeostasis and response to stress.
- Associated diseases and disorders
HnRNP A1 is notably related to neurodegenerative diseases like Amyotrophic Lateral Sclerosis (ALS) and certain types of cancer. Abnormalities in its expression or mutations can lead to disrupted cellular processes contributing to disease pathology. In ALS dysregulation of hnRNP A1 affects neuronal RNA metabolism and is associated with the TDP-43 protein a marker of neurodegeneration. In cancer hnRNP A1 influences tumor development through its role in alternative splicing and gene expression modification linking it to oncogenic signaling pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Dot Blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-citrulline-r140-antibody-epr20178-bsa-and-azide-free-ab251488--dot-blot-img1495.jpg "Dot Blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)")
Dot Blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)
This data was developed using Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] ab208030, the same antibody clone in a different buffer formulation.Dot blot analysis of hnRNP A1 (citrulline R140) labeled with Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] ab208030 at 1/1000 dilution.Lane 1: hnRNP A1 (citrulline R140) peptide.Lane 2: hnRNP A1 (citrulline R140) noncitrulline peptide.
Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody. Blocking and dilution buffer: 5% NFDM/TBST.Exposure time : 3 minutes.![Western blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-a1-citrulline-r140-antibody-epr20178-bsa-and-azide-free-ab251488--western-blot-img12236.jpg "Western blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)")
Western blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] - BSA and Azide free (ab251488)
This data was developed using Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] ab208030, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] (Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] ab208030) at 1/1000 dilution
Lane 1: Empty vector (vector control) transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
Lane 2: HEK-293T whole cell lysate transfected with GFP tagged PADI2 and treated with 10mM CaCl2 for 2h, then 10uM Ionomycin for 2h at 10 µg
Lane 3: Empty vector (vector control) transfected HEK-293T whole cell lysate at 10 µg
Lane 4: HEK-293T whole cell lysate transfected with GFP tagged PADI4 and treated with 10mM CaCl2 for 2h, then 10uM Ionomycin for 2h at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 38 kDa
Observed band size: 34 kDa
Exposure time: 5s
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