AB208027

Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal hnRNP A1 citrulline R88 + R92 antibody. Suitable for IP, Dot, WB, Flow Cyt (Intra) and reacts with Human, Synthetic peptide samples. Cited in 2 publications.

View Alternative Names

HNRPA1, HNRNPA1, Heterogeneous nuclear ribonucleoprotein A1, hnRNP A1, Helix-destabilizing protein, Single-strand RNA-binding protein, hnRNP core protein A1

4 Images

Flow Cytometry (Intracellular) - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] (AB208027)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] (AB208027)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with PADI4 expression vector, then treated with 10 mM CaCl2 and 10 μM Ionomycin for 2 hours, labeling hnRNP A1 with ab208027 at 1/5000 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 647) (ab150079) at 1/2000 dilution was used as the secondary antibody.

The CaCl2 and Ionomycin treated GFP positive cell population gives a positive signal. (Right panel, Q2).

Supplier Data

Immunoprecipitation - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] (AB208027)

hnRNP A1 was immunoprecipitated from 0.35 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) with ab208027 at 1/30 dilution, whole cell lysate. Western blot was performed from the immunoprecipitate using ab208027 at 1/10000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HEK-293T transfected with PADI4 expression vector, then treated with 10 mM CaCl₂ and 10μM Ionomycin for 2 hours whole cell lysate 10 μg (Input).

Lane 2 : ab208027 IP in HEK-293T transfected with PADI4 expression vector, then treated with 10 mM CaCl₂ and 10 μM Ionomycin for 2 hours whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab208027 in HEK-293T transfected with PADI4 expression vector, then treated with 10 mM CaCl₂ and 10 μM Ionomycin for 2 hours whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] (ab208027)

Predicted band size: 38 kDa

Observed band size: 31 kDa,34 kDa,37 kDa

false

Supplier Data

Western blot - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] (AB208027)

hnRNP A1(Uniprot P09651), isoform A1-A (34kD) is twenty times more abundant than isoform A1-B (39kD). The molecular weights of hnRNP A1L2 (Q32P51), hnRNP A0 (Q13151) and hnRNP A2B1 (P22626) are 34kD, 31kD and 37kD respectively.

PAD enzymes (including PADI2 and PADI4) hydrolyze arginine to form citrulline, Ca²⁺ and ionomycin are also required for this catalytic reaction (PMID : 26360112).

Blocking/Dilution buffer : 5% NFDM/TBST

Exposure times : Lane 1-2 : 1 second; Lane 3-4 : 2 seconds.

All lanes:

Western blot - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] (ab208027) at 1/5000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with empty vector (control) then treated with 10 mM CaCl2 and 10 μM Ionomycin for 2 hours, whole cell lysate at 20 µg

Lane 2:

HEK-293T transfected with a PADI4 expression vector then treated with 10 mM CaCl2 and 10 μM Ionomycin for 2 hours, whole cell lysate at 20 µg

Lane 3:

HEK-293T transfected with an empty vector (control) then treated with 10 mM CaCl2 and 10 μM Ionomycin for 2 hours, whole cell lysate at 20 µg

Lane 4:

HEK-293T transfected with a PADI2 expression vector then treated with 10 mM CaCl2 and 10 μM Ionomycin for 2 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 38 kDa

Observed band size: 31 kDa,37 kDa,39 kDa

true

Supplier Data

Dot Blot - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] (AB208027)

Dot Blot analysis of hnRNP A1 (citrulline R88 + R92) labeled with ab208027 at 1/1000 dilution.

Lane 1 : hnRNP A1 (citrulline R88/92) peptide.

Lane 2 : hnRNP A3 (citrulline R109/113) peptide.

Lane 3 : hnRNP A0 (citrulline R81/85) peptide.

Lane 4 : hnRNP A2B1 (citrulline R95/99) peptide.

Lane 5 : CCDC51 (citrulline R142) peptide.

Lane 6 : hnRNP A1 non-citrulline peptide.

Lane 7 : hnRNP A1 (citrulline R88) peptide.

Lane 8 : hnRNP A1 (citrulline R92) peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 30 seconds.

Carrier free

Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20175

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), Dot, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody cross reacts with hnRNP A1L2 (citrulline R88/92), hnRNP A0 (citrulline R81/85) and hnRNP A2B1 (citrulline R95/99).

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) also known just as hnRNP A1 is a multifunctional protein that plays important roles in RNA processing mechanisms. It is a member of the hnRNP protein family involved in the packaging of nascent pre-mRNA. hnRNP A1 has a molecular mass of approximately 34-38 kDa and shows a widespread expression in various cells and tissues. This protein is recognized for its ability to bind to RNA molecules contributing to their proper processing splicing and transport within the cellular context.

Biological function summary

HnRNP A1 is involved in RNA binding and splicing contributing to the formation of spliceosomes the complexes responsible for pre-mRNA splicing. The protein aids in alternative splicing influencing mRNA diversity and stability. hnRNP A1 interacts with other proteins in the hnRNP family remodeling ribonucleoprotein complexes and affecting their functions. These interactions ensure proper mRNA maturation impacting gene expression regulation within the cell.

Pathways

HnRNP A1 is integral to critical cellular mechanisms such as the regulation of mRNA transport and splicing. It influences the DNA damage response pathway by modulating gene expression required for cellular repair and stability. hnRNP A1 also cooperates with other RNA binding proteins like the splicing factors SR proteins to orchestrate the splicing machinery. These interactions are pivotal in maintaining cellular homeostasis and response to stress.

HnRNP A1 is notably related to neurodegenerative diseases like Amyotrophic Lateral Sclerosis (ALS) and certain types of cancer. Abnormalities in its expression or mutations can lead to disrupted cellular processes contributing to disease pathology. In ALS dysregulation of hnRNP A1 affects neuronal RNA metabolism and is associated with the TDP-43 protein a marker of neurodegeneration. In cancer hnRNP A1 influences tumor development through its role in alternative splicing and gene expression modification linking it to oncogenic signaling pathways.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and modulation of splice site selection (PubMed : 17371836). Plays a role in the splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808). Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1 (PubMed : 31498791). May bind to specific miRNA hairpins (PubMed : 28431233).. (Microbial infection) May play a role in HCV RNA replication.. (Microbial infection) Cleavage by Enterovirus 71 protease 3C results in increased translation of apoptosis protease activating factor APAF1, leading to apoptosis.

See full target information HNRNPA1 citR88 + R92

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bladder cancer (Amsterdam, Netherlands) 9:29-40 PubMed38994477

2023

SH3BP5-AS1/IGF2BP2/VDAC2 Axis Promotes the Apoptosis and Ferroptosis of Bladder Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Shao,Yunhui Chan,Rong Zhao

Science advances 8:eabq7289 PubMed36563164

2022

MALAT1 modulates alternative splicing by cooperating with the splicing factors PTBP1 and PSF.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Miao,Fan Wu,Yu Li,Chenyu Qin,Yongyun Zhao,Mingfeng Xie,Hongyuan Dai,Hong Yao,Haoyang Cai,Qianhong Wang,Xu Song,Ling Li

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175]

Flow Cytometry (Intracellular) - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] (AB208027)

Immunoprecipitation - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] (AB208027)

Western blot - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] (AB208027)

Dot Blot - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] (AB208027)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise