AB183654

Anti-hnRNP A2B1 antibody

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(6 Publications)

Rabbit Polyclonal hnRNP A2B1 antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HNRNPA2B1 aa 100-350.

View Alternative Names

HNRPA2B1, HNRNPA2B1, Heterogeneous nuclear ribonucleoproteins A2/B1, hnRNP A2/B1

10 Images

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A2B1 antibody (AB183654)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A2B1 antibody (AB183654)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling hnRNP A2B1 protein at endoplasmic reticulum and nucleus using ab183654 at a 1/500 dilution (Green). alpha Tubulin, a cytoskeleton marker, stained by an anti-alpha Tubulin antibody at a 1/1000 dilution (Red).
Scale bar = 10 µM

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody (AB183654)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody (AB183654)

Immunohistochemical analysis of paraffin-embedded mouse duodenum tissue stained for hnRNP A2B1 protein at cytosol and nucleus with ab183654 at a 1/500 dilution.

Antigen retrieval : EDTA based, pH 8.0 buffer, 15min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody (AB183654)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody (AB183654)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for hnRNP A2B1 protein at nucleus with ab183654 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody (AB183654)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody (AB183654)

Immunohistochemical analysis of paraffin-embedded mouse stomach, kidney, brain, lung tissue stained for hnRNP A2B1 protein with ab183654 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody (AB183654)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody (AB183654)

Immunohistochemical analysis of paraffin-embedded mouse intestine tissue stained for hnRNP A2B1 protein at nucleus with ab183654 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody (AB183654)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody (AB183654)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for hnRNP A2B1 protein at nucleus with ab183654 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody (AB183654)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody (AB183654)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for hnRNP A2B1 protein at cytoplasm and nucleus with ab183654 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody (AB183654)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody (AB183654)

Immunohistochemical analysis of paraffin-embedded rat stomach, kidney, brain, lung tissue stained for hnRNP A2B1 protein with ab183654 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Western blot - Anti-hnRNP A2B1 antibody (AB183654)

WB

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Western blot - Anti-hnRNP A2B1 antibody (AB183654)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-hnRNP A2B1 antibody (ab183654) at 1/10000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 37 kDa

false

Western blot - Anti-hnRNP A2B1 antibody (AB183654)

WB

Supplier Data

Western blot - Anti-hnRNP A2B1 antibody (AB183654)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-hnRNP A2B1 antibody (ab183654) at 1/10000 dilution

Lane 1:

Neuro2A whole cell extracts at 30 µg

Lane 2:

Rat-2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

Immunogen

Recombinant Fragment Protein within Human HNRNPA2B1 aa 100-350. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HnRNP A2B1 sometimes referred to as heterogeneous nuclear ribonucleoprotein A2/B1 is a multifunctional protein involved in RNA binding and processing. It has a molecular mass of about 38-43 kDa. The protein is expressed in various tissues with high expression levels observed in the brain and liver. hnRNP A2B1 participates in a range of RNA-related processes playing a significant role in mRNA metabolism and transport.

Biological function summary

HnRNP A2B1 contributes to alternative splicing RNA stability and translational regulation. This protein typically associates with complex assemblies that manage RNA processing and transport mechanisms. It interacts with other proteins within the heterogeneous nuclear ribonucleoprotein family collaborating in the regulation of mRNA cycling between the nucleus and cytoplasm affecting gene expression patterns.

Pathways

HnRNP A2B1 exhibits critical involvement in the mRNA splicing pathway and RNA transport pathways. It frequently interacts with proteins such as hnRNP A1 and hnRNP C where they collectively influence pre-mRNA processing and splicing dictating proper RNA maturation and cell function. These pathways are important for maintaining cellular homeostasis and responding to cellular signals.

HnRNP A2B1 is linked to neurodegenerative diseases and certain cancers. Abnormal expression or mutations of hnRNP A2B1 are associated with Alzheimer's disease implicating the protein in amyloid precursor protein processing. Additionally dysregulation of this protein shows correlation with breast cancer progression. Proteins like tau and other hnRNPs connect with hnRNP A2B1 in these conditions suggesting a significant role in pathological states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Heterogeneous nuclear ribonucleoprotein (hnRNP) that associates with nascent pre-mRNAs, packaging them into hnRNP particles. The hnRNP particle arrangement on nascent hnRNA is non-random and sequence-dependent and serves to condense and stabilize the transcripts and minimize tangling and knotting. Packaging plays a role in various processes such as transcription, pre-mRNA processing, RNA nuclear export, subcellular location, mRNA translation and stability of mature mRNAs (PubMed : 19099192). Forms hnRNP particles with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleus. Involved in transport of specific mRNAs to the cytoplasm in oligodendrocytes and neurons : acts by specifically recognizing and binding the A2RE (21 nucleotide hnRNP A2 response element) or the A2RE11 (derivative 11 nucleotide oligonucleotide) sequence motifs present on some mRNAs, and promotes their transport to the cytoplasm (PubMed : 10567417). Specifically binds single-stranded telomeric DNA sequences, protecting telomeric DNA repeat against endonuclease digestion (By similarity). Also binds other RNA molecules, such as primary miRNA (pri-miRNAs) : acts as a nuclear 'reader' of the N6-methyladenosine (m6A) mark by specifically recognizing and binding a subset of nuclear m6A-containing pri-miRNAs. Binding to m6A-containing pri-miRNAs promotes pri-miRNA processing by enhancing binding of DGCR8 to pri-miRNA transcripts (PubMed : 26321680). Involved in miRNA sorting into exosomes following sumoylation, possibly by binding (m6A)-containing pre-miRNAs (PubMed : 24356509). Acts as a regulator of efficiency of mRNA splicing, possibly by binding to m6A-containing pre-mRNAs (PubMed : 26321680). Plays a role in the splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808). Also plays a role in the activation of the innate immune response (PubMed : 31320558). Mechanistically, senses the presence of viral DNA in the nucleus, homodimerizes and is demethylated by JMJD6 (PubMed : 31320558). In turn, translocates to the cytoplasm where it activates the TBK1-IRF3 pathway, leading to interferon alpha/beta production (PubMed : 31320558).. (Microbial infection) Involved in the transport of HIV-1 genomic RNA out of the nucleus, to the microtubule organizing center (MTOC), and then from the MTOC to the cytoplasm : acts by specifically recognizing and binding the A2RE (21 nucleotide hnRNP A2 response element) sequence motifs present on HIV-1 genomic RNA, and promotes its transport.

See full target information HNRNPA2B1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 31:e70532 PubMed40776395

2025

Kapβ2 Reverses Sevoflurane-Induced Hydrogel Phase Transition of hnRNPA2/B1-SG in Hypoxic Primary Rat Hippocampal Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Zhang,Xinyi Wang,Feiyu Jia,Chenyi Yang,Zixuan Wang,Huihui Liao,Lin Zhang,Xi Xin,Haiyun Wang

The Journal of biological chemistry 301:110303 PubMed40447192

2025

Post-transcriptional regulation of H19 by mA reader proteins: H19 stability versus miR-675 biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Samarjit Jana,Abhishek Chowdhury,Kumaravel Somasundaram

Aging cell 24:e14418 PubMed39610020

2024

Knockdown of RUVBL2 improves hnRNPA2/B1-stress granules dynamics to inhibit perioperative neurocognitive disorders in aged mild cognitive impairment rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zixuan Wang,Chenyi Yang,Xinyi Wang,Huihui Liao,Xing Liu,Huan Liu,Miao Zhang,Lin Zhang,Haiyun Wang

Journal of extracellular vesicles 12:e12366 PubMed37885043

2023

Culture conditions greatly impact the levels of vesicular and extravesicular Ago2 and RNA in extracellular vesicle preparations.

Applications

Unspecified application

Species

Unspecified reactive species

Lizandra Jimenez,Bahnisikha Barman,Youn Jae Jung,Lauren Cocozza,Evan Krystofiak,Cherie Saffold,Kasey C Vickers,John T Wilson,T Renee Dawson,Alissa M Weaver

Nature communications 14:2094 PubMed37055411

2023

Extracellular vesicles engineering by silicates-activated endothelial progenitor cells for myocardial infarction treatment in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Yu,Hekai Li,Zhaowenbin Zhang,Peier Chen,Ling Wang,Xianglin Fan,Xiaodong Ning,Yuxuan Pan,Feiran Zhou,Xinyi Hu,Jiang Chang,Caiwen Ou

International journal of clinical and experimental 11:4967-4976 PubMed31949573

2018

Loss of Wnt7a expression correlates with tumor progression and poor prognosis in colorectal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Se Min Jang,Young Chan Wi,Yeseul Kim,Seong Sik Bang,Jongmin Sim,Byung Kyu Ahn,Kang Hong Lee,Su-Jin Shin,Kiseok Jang,Seung Sam Paik

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