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Rabbit Recombinant Monoclonal hnRNP C1 + C2/HNRNPC antibody. Suitable for WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra) and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP C1/C2 antibody [9G1] (AB314004), expandable thumbnail
  • Immunoprecipitation - Anti-hnRNP C1/C2 antibody [9G1] (AB314004), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-hnRNP C1/C2 antibody [9G1] (AB314004), expandable thumbnail
  • Western blot - Anti-hnRNP C1/C2 antibody [9G1] (AB314004), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [9G1] (AB314004), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.1% PBS, 0.88% Sodium chloride

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFIPFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/5000.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50.00000 - 1/200.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20.00000 - 1/200.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200.00000 - 1/1000.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Target data

Function

Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides (PubMed:8264621). May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671).

Alternative names

HNRPC, HNRNPC, Heterogeneous nuclear ribonucleoproteins C1/C2, hnRNP C1/C2

Recommended products

Rabbit Recombinant Monoclonal hnRNP C1 + C2/HNRNPC antibody. Suitable for WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
9G1
Purification technique
Affinity purification
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

HnRNP C1/C2 also known as heterogeneous nuclear ribonucleoprotein C comprises two alternatively spliced proteins C1 protein and C2 protein which have molecular weights of approximately 39 kDa and 41 kDa respectively. These proteins function mainly in the nucleus and are expressed ubiquitously in various tissues. They play a fundamental role in mRNA processing including pre-mRNA splicing polyadenylation and stabilization. As components of the hnRNP complex they bind to nascent RNA transcripts to regulate their maturation and transport.

Biological function summary

HnRNP C1/C2 participates in the assembly of large ribonucleoprotein complexes that are essential for mRNA metabolism. It acts as a core component of the spliceosome machinery where it influences the alternative splicing of pre-mRNA. Apart from its structural role hnRNP C1/C2 may contribute to the proper assembly and functioning of splicing factors and other proteins such as 4f4 within the nucleus suggesting its important role in gene expression regulation.

Pathways

HnRNP C1/C2 is involved in the RNA processing pathway directly impacting mRNA export and stability. By interacting with other proteins such as serine/arginine-rich splicing factors it modulates the splicing accuracy and efficiency of various transcripts. This participation in the spliceosome pathway emphasizes its importance in ensuring efficient gene expression and processing. hnRNP C1/C2 might also interact with various transcription machinery components linking RNA processing to transcriptional regulation.

Associated diseases and disorders

HnRNP C1/C2 links to neurological diseases and certain cancers. In neurological disorders misregulation of hnRNP C1/C2 can result in aberrant mRNA splicing which may contribute to the pathogenesis of diseases like spinal muscular atrophy. Its involvement in cancer is related to its ability to modulate gene expression and cellular proliferation often observed in tumors with disrupted mRNA processing pathways. HnRNP C1/C2 interactions with proteins such as the tumor suppressor p53 may influence cell cycle regulation and contribute to tumor progression.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP C1/C2 antibody [9G1] (ab314004), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP C1/C2 antibody [9G1] (ab314004)

    Immunofluorescence staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells with ab314004 at 1:22, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

  • Immunoprecipitation - Anti-hnRNP C1/C2 antibody [9G1] (ab314004), expandable thumbnail

    Immunoprecipitation - Anti-hnRNP C1/C2 antibody [9G1] (ab314004)

    Immunoprecipitating hnRNP C1/C2 in HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 1: Rabbit control IgG instead of ab314004 in HeLa whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: ab314004 (3μg) + HeLa whole cell lysate (500μg)
    Lane 3: HeLa whole cell lysate (20μg)

    All lanes: Immunoprecipitation - Anti-hnRNP C1/C2 antibody [9G1] (ab314004) at 3 µg

    Predicted band size: 33 kDa

  • Flow Cytometry (Intracellular) - Anti-hnRNP C1/C2 antibody [9G1] (ab314004), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-hnRNP C1/C2 antibody [9G1] (ab314004)

    Overlay histogram showing MCF7 (Human breast adenocarcinoma cell line) cells stained with ab314004 (pink line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

  • Western blot - Anti-hnRNP C1/C2 antibody [9G1] (ab314004), expandable thumbnail

    Western blot - Anti-hnRNP C1/C2 antibody [9G1] (ab314004)

    All lanes: Western blot - Anti-hnRNP C1/C2 antibody [9G1] (ab314004) at 0.66 µg/mL

    Lane 1: HeLa whole cell lysate

    Lane 2: K562 whole cell lysate

    Lane 3: HEK293T whole cell lysate

    Lane 4: MCF7 whole cell lysate

    Lane 5: Raji whole cell lysate

    Lane 6: Jurkat whole cell lysate

    Lane 7: HepG2 whole cell lysate

    Secondary

    All lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution

    Observed band size: 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [9G1] (ab314004), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [9G1] (ab314004)

    Immunohistochemical analysis of ab314004 diluted at 1:66.35 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product protocols

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