Anti-hnRNP C1/C2 antibody [9G1]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-hnRNP C1/C2 antibody [9G1] (AB314004)

Overlay Peak curve showing MCF7 cells stained with ab314004 (red line) at 1/50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. T

hen 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1/200 dilution for 35min at 4°C.

Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [9G1] (AB314004)

IHC image of ab314004 diluted at 1/300 and staining in paraffin-embedded human breast cancer performed on a Leica Bond^™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [9G1] (AB314004)

IHC image of ab314004 diluted at 1/300 and staining in paraffin-embedded human kidney tissue performed on a Leica Bond^™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody

• IP

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Immunoprecipitation - Anti-hnRNP C1/C2 antibody [9G1] (AB314004)

Immunoprecipitating HNRNPC in Hela whole cell lysate

Lane 1 : Rabbit control IgG instead of ab314004 in Hela whole cell lysate
Lane 2 : ab314004（3µg）+ Hela whole cell lysate（500µg）
Lane 3 : Hela whole cell lysate(20µg)

For western blotting, Goat polyclonal to rabbit IgG antibody was used as the secondary antibody (1/50000)

All lanes:

Immunoprecipitation - Anti-hnRNP C1/C2 antibody [9G1] (ab314004)

Lane 1:

Rabbit control IgG instead of ab314004 in Hela whole cell lysate

Lane 2:

ab314004 (3ug) and Hela whole cell lysate (500ug)

Lane 3:

Hela whole cell lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG antibody was used as the secondary antibody at 1/50000 dilution

false

• WB

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Western blot - Anti-hnRNP C1/C2 antibody [9G1] (AB314004)

All lanes:

Western blot - Anti-hnRNP C1/C2 antibody [9G1] (ab314004) at 1/1000 dilution

Lane 1:

HeLa cells

Lane 2:

293 cells

Lane 3:

Jurkat cells

Lane 4:

Raji cells

Lane 5:

MCF7 cells

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 28 kDa,33 kDa,34 kDa,36 kDa

Observed band size: 42 kDa

false