Rabbit Recombinant Monoclonal hnRNP C1 + C2/HNRNPC antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (AB75822), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-c1-c2-antibody-ep3034y-ab75822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428427.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (AB75822)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (AB75822), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-c1-c2-antibody-ep3034y-ab75822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428428.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (AB75822)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (AB75822), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-c1-c2-antibody-ep3034y-ab75822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428429.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (AB75822)")
![Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (AB75822), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-c1-c2-antibody-ep3034y-ab75822--western-blot-img428426.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (AB75822)")
![Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (AB75822), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-c1-c2-antibody-ep3034y-ab75822--western-blot-img428425.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (AB75822)")
Publications
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- PloS one 13:e02011032018T cell LFA-1-induced proinflammatory mRNA stabilization is mediated by the p38 pathway kinase MK2 in a process regulated by hnRNPs C, H1 and K.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGautham K Rao et. al.PubMed 30048492
- Journal of virology 86:7180-912012Evasion of the innate immune response: the Old World alphavirus nsP2 protein induces rapid degradation of Rpb1, a catalytic subunit of RNA polymerase II.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | Flow Cyt | IP | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended |
| Rat | Tested | Tested | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/800 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/800 | Notes - |
Species Rat | Dilution info 1/800 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 | Notes - |
Species Mouse | Dilution info 1/10000 | Notes - |
Species Rat | Dilution info 1/10000 | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides (PubMed:8264621). May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671).
Alternative names
HNRPC, HNRNPC, Heterogeneous nuclear ribonucleoproteins C1/C2, hnRNP C1/C2
Recommended products
Rabbit Recombinant Monoclonal hnRNP C1 + C2/HNRNPC antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP3034Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
This product has switched from a hybridoma to recombinant production method on 23rd February 2024.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HnRNP C1/C2 also known as heterogeneous nuclear ribonucleoprotein C comprises two alternatively spliced proteins C1 protein and C2 protein which have molecular weights of approximately 39 kDa and 41 kDa respectively. These proteins function mainly in the nucleus and are expressed ubiquitously in various tissues. They play a fundamental role in mRNA processing including pre-mRNA splicing polyadenylation and stabilization. As components of the hnRNP complex they bind to nascent RNA transcripts to regulate their maturation and transport.
- Biological function summary
HnRNP C1/C2 participates in the assembly of large ribonucleoprotein complexes that are essential for mRNA metabolism. It acts as a core component of the spliceosome machinery where it influences the alternative splicing of pre-mRNA. Apart from its structural role hnRNP C1/C2 may contribute to the proper assembly and functioning of splicing factors and other proteins such as 4f4 within the nucleus suggesting its important role in gene expression regulation.
- Pathways
HnRNP C1/C2 is involved in the RNA processing pathway directly impacting mRNA export and stability. By interacting with other proteins such as serine/arginine-rich splicing factors it modulates the splicing accuracy and efficiency of various transcripts. This participation in the spliceosome pathway emphasizes its importance in ensuring efficient gene expression and processing. hnRNP C1/C2 might also interact with various transcription machinery components linking RNA processing to transcriptional regulation.
- Associated diseases and disorders
HnRNP C1/C2 links to neurological diseases and certain cancers. In neurological disorders misregulation of hnRNP C1/C2 can result in aberrant mRNA splicing which may contribute to the pathogenesis of diseases like spinal muscular atrophy. Its involvement in cancer is related to its ability to modulate gene expression and cellular proliferation often observed in tumors with disrupted mRNA processing pathways. HnRNP C1/C2 interactions with proteins such as the tumor suppressor p53 may influence cell cycle regulation and contribute to tumor progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-c1-c2-antibody-ep3034y-ab75822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428427.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)
Immunohistochemical analysis of paraffin-embedded Human breast cancer labeling hnRNP C1/C2 with ab75822 at 1/800 dilution (0.589 ?g/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab75822 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-c1-c2-antibody-ep3034y-ab75822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428428.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling hnRNP C1/C2 with ab75822 at 1/800 dilution (0.589 ?g/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab75822 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-c1-c2-antibody-ep3034y-ab75822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428429.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling hnRNP C1/C2 with ab75822 at 1/800 dilution (0.589 ?g/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab75822 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-c1-c2-antibody-ep3034y-ab75822--western-blot-img428426.jpg "Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)")
Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822) at 1/100000 dilution
All lanes: MCF7(Human breast adenocarcinoma epithelial cell) whole lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 180s
![Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-c1-c2-antibody-ep3034y-ab75822--western-blot-img428425.jpg "Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)")
Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1, 60 seconds; Lane 2, 5 seconds; Lane 3, 3 seconds.
All lanes: Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822) at 1/10000 dilution
Lane 1: Mouse brain lysate at 15 µg
Lane 2: Rat brain lysate at 15 µg
Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) whole lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
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