Anti-hnRNP C1/C2 antibody [EP3034Y] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] - BSA and Azide free (AB284719)

This data was developed using ab75822, the same antibody clone in a different buffer.

Immunohistochemical analysis of paraffin-embedded Human breast cancer labeling hnRNP C1/C2 with ab75822 at 1/800 dilution (0.589 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab75822 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] - BSA and Azide free (AB284719)

This data was developed using ab75822, the same antibody clone in a different buffer.

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling hnRNP C1/C2 with ab75822 at 1/800 dilution (0.589 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab75822 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] - BSA and Azide free (AB284719)

This data was developed using ab75822, the same antibody clone in a different buffer.

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling hnRNP C1/C2 with ab75822 at 1/800 dilution (0.589 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab75822 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] - BSA and Azide free (AB284719)

This data was developed using ab75822, the same antibody clone in a different buffer.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lane 1, 60 seconds; Lane 2, 5 seconds; Lane 3, 3 seconds.

All lanes:

Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (<a href='/en-us/products/primary-antibodies/hnrnp-c1-c2-antibody-ep3034y-ab75822'>ab75822</a>) at 1/10000 dilution

Lane 1:

Mouse brain lysate at 15 µg

Lane 2:

Rat brain lysate at 15 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) whole lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

• WB

Supplier Data

Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] - BSA and Azide free (AB284719)

This data was developed using ab75822, the same antibody clone in a different buffer.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (<a href='/en-us/products/primary-antibodies/hnrnp-c1-c2-antibody-ep3034y-ab75822'>ab75822</a>) at 1/100000 dilution

All lanes:

MCF7(Human breast adenocarcinoma epithelial cell) whole lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Exposure time: 180s