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AB133607

Anti-hnRNP C1/C2 antibody [EPNCIR152]

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(2 Reviews)

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(25 Publications)

Rabbit Recombinant Monoclonal hnRNP C1 + C2/HNRNPC antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 25 publications.

View Alternative Names

HNRPC, HNRNPC, Heterogeneous nuclear ribonucleoproteins C1/C2, hnRNP C1/C2

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EPNCIR152] (AB133607)
  • IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EPNCIR152] (AB133607)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling hnRNP C1/C2 with ab133607 antibody at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EPNCIR152] (AB133607)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EPNCIR152] (AB133607)

Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labelling hnRNP C1/C2 with ab133607 antibody at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP C1/C2 antibody [EPNCIR152] (AB133607)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP C1/C2 antibody [EPNCIR152] (AB133607)

Immunofluorescence staining of MCF-7 cells with purified ab133607 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

Western blot - Anti-hnRNP C1/C2 antibody [EPNCIR152] (AB133607)
  • WB

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Western blot - Anti-hnRNP C1/C2 antibody [EPNCIR152] (AB133607)

Actual band size : 42 kDa

All lanes:

Western blot - Anti-hnRNP C1/C2 antibody [EPNCIR152] (ab133607) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Lane 5:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 33 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPNCIR152

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific to isoform C2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

This antibody was developed as part of a collaboration between the National Cancer Institute's Center for Cancer Research and the lab of Mirit Aladjem. View antibodies from NCI Center for Cancer Research Collaboration.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HnRNP C1/C2 also known as heterogeneous nuclear ribonucleoprotein C comprises two alternatively spliced proteins C1 protein and C2 protein which have molecular weights of approximately 39 kDa and 41 kDa respectively. These proteins function mainly in the nucleus and are expressed ubiquitously in various tissues. They play a fundamental role in mRNA processing including pre-mRNA splicing polyadenylation and stabilization. As components of the hnRNP complex they bind to nascent RNA transcripts to regulate their maturation and transport.
Biological function summary

HnRNP C1/C2 participates in the assembly of large ribonucleoprotein complexes that are essential for mRNA metabolism. It acts as a core component of the spliceosome machinery where it influences the alternative splicing of pre-mRNA. Apart from its structural role hnRNP C1/C2 may contribute to the proper assembly and functioning of splicing factors and other proteins such as 4f4 within the nucleus suggesting its important role in gene expression regulation.

Pathways

HnRNP C1/C2 is involved in the RNA processing pathway directly impacting mRNA export and stability. By interacting with other proteins such as serine/arginine-rich splicing factors it modulates the splicing accuracy and efficiency of various transcripts. This participation in the spliceosome pathway emphasizes its importance in ensuring efficient gene expression and processing. hnRNP C1/C2 might also interact with various transcription machinery components linking RNA processing to transcriptional regulation.

HnRNP C1/C2 links to neurological diseases and certain cancers. In neurological disorders misregulation of hnRNP C1/C2 can result in aberrant mRNA splicing which may contribute to the pathogenesis of diseases like spinal muscular atrophy. Its involvement in cancer is related to its ability to modulate gene expression and cellular proliferation often observed in tumors with disrupted mRNA processing pathways. HnRNP C1/C2 interactions with proteins such as the tumor suppressor p53 may influence cell cycle regulation and contribute to tumor progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed : 8264621). Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed : 12509468, PubMed : 16010978, PubMed : 7567451, PubMed : 8264621). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides (PubMed : 8264621). May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing (PubMed : 25719671).
See full target information HNRNPC
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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 56: PubMed40937577

2025

CDC5L binds to ELAVL1 to inhibit pyroptosis in hepatocellular carcinoma through the Caspase 3/GSDME signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Liang,Zhongcheng Zhu,Yangshuo Tang,Shuhua Zhou,Moyan Xiao,Xuejun Gong,Ke Ye

Frontiers in oncology 15:1516867 PubMed39990687

2025

Expression and prognostic significance of the m6A RNA methylation regulator HNRNPC in HNSCC.

Applications

Unspecified application

Species

Unspecified reactive species

Yulin Zhang,Yixu Wang,Jilin Peng,Kun Zhao,Ling Li,Yuan Zhang,Ziyu Zhai,Sijie Yuan,Shichao Li,Fanglei Ye,Le Wang

Food science & nutrition 12:9238-9251 PubMed39619978

2024

RNA m6A methylation regulatory mechanism of resveratrol in premature senescence cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Zhang,Chenyu Zhu,Luyun Zhang,Luyi Tan,Wenli Cheng,Min Li,Xingtan Zhang,Wenjuan Zhang,Wenji Zhang

The Journal of biological chemistry 300:105591 PubMed38141769

2023

LncNFYB promotes the proliferation of rheumatoid arthritis fibroblast-like synoviocytes via LncNFYB/ANXA2/ERK1/2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shibai Xiao,Qingqing Ouyang,Yi Feng,Xiaoxi Lu,Yipeng Han,Hao Ren,Qin Huang,Jinjun Zhao,Changhong Xiao,Min Yang

Cancer biology & therapy 24:2246203 PubMed37599448

2023

Nucleo-cytoplasmic shuttling of 14-3-3 epsilon carrying hnRNP C promotes autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Manlan Guo,Minyi He,Yi Zhang,Weiwen Liu,Min Qi,Zhifeng Liu,Guozhong Yi,Shengze Deng,Yaomin Li,Xuegang Sun,Liang Zhao,Tengxiang Chen,Yawei Liu

Molecular carcinogenesis 62:1846-1859 PubMed37589421

2023

N6-methyladenosine methylation regulatory pattern of pulmonary lymphoepithelioma-like carcinoma based on exosomal transcriptome analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengge Yu,Yiyun Pan,Huahua Li,Xiaomei Liu,Zhengcong Chen,Hailong Chen,Shudong Ma,Wen Zeng

Molecular medicine (Cambridge, Mass.) 29:103 PubMed37528369

2023

HNRNPC promotes collagen fiber alignment and immune evasion in breast cancer via activation of the VIRMA-mediated TFAP2A/DDR1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Lian,Shuxun Yan,Jiayi Li,Zhengyang Bai,Jinping Li

Molecules (Basel, Switzerland) 28: PubMed37298909

2023

New Functions of Intracellular LOXL2: Modulation of RNA-Binding Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Pilar Eraso,María J Mazón,Victoria Jiménez,Patricia Pizarro-García,Eva P Cuevas,Jara Majuelos-Melguizo,Jesús Morillo-Bernal,Amparo Cano,Francisco Portillo

The Journal of cell biology 222: PubMed37200023

2023

Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters.

Applications

Unspecified application

Species

Unspecified reactive species

Femmy C Stempels,Muwei Jiang,Harry M Warner,Magda-Lena Moser,Maaike H Janssens,Sjors Maassen,Iris H Nelen,Rinse de Boer,William F Jiemy,David Knight,Julian Selley,Ronan O'Cualain,Maksim V Baranov,Thomas C Q Burgers,Roberto Sansevrino,Dragomir Milovanovic,Peter Heeringa,Matthew C Jones,Rifka Vlijm,Martin Ter Beest,Geert van den Bogaart

International journal of molecular sciences 23: PubMed36012545

2022

Dynamic Alteration Profile and New Role of RNA m6A Methylation in Replicative and HO-Induced Premature Senescence of Human Embryonic Lung Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Wu,Luyun Zhang,Caiyun Lai,Xinyue Peng,Susu Yu,Cheng Zhou,Bo Zhang,Wenjuan Zhang
View all publications
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