Rabbit Recombinant Monoclonal hnRNP C1 + C2/HNRNPC antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Images
![Western blot - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (AB248575), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-c1-c2-antibody-epncir152-bsa-and-azide-free-ab248575--western-blot-img113753.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (AB248575)")
![Immunocytochemistry/ Immunofluorescence - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (AB248575), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-c1-c2-antibody-epncir152-bsa-and-azide-free-ab248575--immunocytochemistry-immunofluorescence-img15189.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (AB248575)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (AB248575), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-c1-c2-antibody-epncir152-bsa-and-azide-free-ab248575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73658.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (AB248575)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (AB248575), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-c1-c2-antibody-epncir152-bsa-and-azide-free-ab248575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74423.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (AB248575)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Version
Target data
Function
Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides (PubMed:8264621). May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671).
Alternative names
HNRPC, HNRNPC, Heterogeneous nuclear ribonucleoproteins C1/C2, hnRNP C1/C2
Recommended products
Rabbit Recombinant Monoclonal hnRNP C1 + C2/HNRNPC antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPNCIR152
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody is specific to isoform C2.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248575 is the carrier-free version of Anti-hnRNP C1/C2 antibody [EPNCIR152] ab133607.
This antibody was developed as part of a collaboration between the National Cancer Institute's Center for Cancer Research and the lab of Mirit Aladjem. View antibodies from NCI Center for Cancer Research Collaboration.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HnRNP C1/C2 also known as heterogeneous nuclear ribonucleoprotein C comprises two alternatively spliced proteins C1 protein and C2 protein which have molecular weights of approximately 39 kDa and 41 kDa respectively. These proteins function mainly in the nucleus and are expressed ubiquitously in various tissues. They play a fundamental role in mRNA processing including pre-mRNA splicing polyadenylation and stabilization. As components of the hnRNP complex they bind to nascent RNA transcripts to regulate their maturation and transport.
- Biological function summary
HnRNP C1/C2 participates in the assembly of large ribonucleoprotein complexes that are essential for mRNA metabolism. It acts as a core component of the spliceosome machinery where it influences the alternative splicing of pre-mRNA. Apart from its structural role hnRNP C1/C2 may contribute to the proper assembly and functioning of splicing factors and other proteins such as 4f4 within the nucleus suggesting its important role in gene expression regulation.
- Pathways
HnRNP C1/C2 is involved in the RNA processing pathway directly impacting mRNA export and stability. By interacting with other proteins such as serine/arginine-rich splicing factors it modulates the splicing accuracy and efficiency of various transcripts. This participation in the spliceosome pathway emphasizes its importance in ensuring efficient gene expression and processing. hnRNP C1/C2 might also interact with various transcription machinery components linking RNA processing to transcriptional regulation.
- Associated diseases and disorders
HnRNP C1/C2 links to neurological diseases and certain cancers. In neurological disorders misregulation of hnRNP C1/C2 can result in aberrant mRNA splicing which may contribute to the pathogenesis of diseases like spinal muscular atrophy. Its involvement in cancer is related to its ability to modulate gene expression and cellular proliferation often observed in tumors with disrupted mRNA processing pathways. HnRNP C1/C2 interactions with proteins such as the tumor suppressor p53 may influence cell cycle regulation and contribute to tumor progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (ab248575), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-c1-c2-antibody-epncir152-bsa-and-azide-free-ab248575--western-blot-img113753.jpg "Western blot - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (ab248575)")
Western blot - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (ab248575)
This data was developed using Anti-hnRNP C1/C2 antibody [EPNCIR152] ab133607, the same antibody clone in a different buffer formulation.
Actual band size: 42 kDa.
All lanes: Western blot - Anti-hnRNP C1/C2 antibody [EPNCIR152] (Anti-hnRNP C1/C2 antibody [EPNCIR152] ab133607) at 1/10000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: MCF7 cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
Lane 4: K562 cell lysate at 10 µg
Lane 5: 293T cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 33 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (ab248575), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-c1-c2-antibody-epncir152-bsa-and-azide-free-ab248575--immunocytochemistry-immunofluorescence-img15189.jpg "Immunocytochemistry/ Immunofluorescence - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (ab248575)")
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (ab248575)
This data was developed using Anti-hnRNP C1/C2 antibody [EPNCIR152] ab133607, the same antibody clone in a different buffer formulation. Immunofluorescence staining of MCF-7 cells with purified Anti-hnRNP C1/C2 antibody [EPNCIR152] ab133607 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (ab248575), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-c1-c2-antibody-epncir152-bsa-and-azide-free-ab248575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73658.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (ab248575)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (ab248575)
This data was developed using Anti-hnRNP C1/C2 antibody [EPNCIR152] ab133607, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling hnRNP C1/C2 with Anti-hnRNP C1/C2 antibody [EPNCIR152] ab133607 antibody at a dilution of 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (ab248575), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-c1-c2-antibody-epncir152-bsa-and-azide-free-ab248575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74423.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (ab248575)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EPNCIR152] - BSA and Azide free (ab248575)
This data was developed using Anti-hnRNP C1/C2 antibody [EPNCIR152] ab133607, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labelling hnRNP C1/C2 with Anti-hnRNP C1/C2 antibody [EPNCIR152] ab133607 antibody at a dilution of 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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