Rabbit Polyclonal hnRNP D/AUF1 antibody. Suitable for IP, ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 27 publications. Immunogen corresponding to Synthetic Peptide within Human HNRNPD.
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- Molecular medicine (Cambridge, Mass.) 29:1622023The linear ANRIL transcript P14AS regulates the NF-κB signaling to promote colon cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanru Ma,Junhua HuPubMed 38041015
- FEBS open bio 12:82-942021Cpeb1 expression is post-transcriptionally regulated by AUF1, CPEB1, and microRNAs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSouichi Oe,Shinichi Hayashi,Susumu Tanaka,Taro Koike,Yukie Hirahara,Rio Kakizaki,Sumika Sakamoto,Yasuko Noda,Hisao Yamada,Masaaki KitadaPubMed 34480525
- Cell reports 35:1091562021Inhibiting an RBM39/MLL1 epigenomic regulatory complex with dominant-negative peptides disrupts cancer cell transcription and proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPavan Kumar Puvvula,Yao Yu,Kaelan Renaldo Sullivan,Henok Eyob,Julian Rosenberg,Alana Welm,Chad Huff,Anne M MoonPubMed 34077726
- Frontiers in oncology 11:6218252021Novel Cell-Penetrating Peptides Derived From Scaffold-Attachment- Factor A Inhibits Cancer Cell Proliferation and Survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPavan Kumar Puvvula,Anne M MoonPubMed 33859938
- International journal of molecular sciences 21:2020Regulation of Fetal Genes by Transitions among RNA-Binding Proteins during Liver Development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesToru Suzuki et. al.PubMed 33297405
- Molecular therapy : the journal of the American Society of Gene Therapy 28:2488-25022020lncRNA ZNF649-AS1 Induces Trastuzumab Resistance by Promoting ATG5 Expression and Autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingli Han,Xueke Qian,Hui Cao,Fang Wang,Xiangke Li,Na Han,Xue Yang,Yunqing Yang,Dongwei Dou,Jianguo Hu,Wei Wang,Jing Han,Fan Zhang,Huaying DongPubMed 32735773
- Molecular cancer 19:422020Characterization of novel LncRNA P14AS as a protector of ANRIL through AUF1 binding in human cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanru Ma et. al.PubMed 32106863
- Molecular cancer 19:262020Exosome-mediated lncRNA AFAP1-AS1 promotes trastuzumab resistance through binding with AUF1 and activating ERBB2 translation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingli Han et. al.PubMed 32020881
- Aging and disease 11:17-302020Angelica Sinensis Polysaccharide Suppresses Epithelial-Mesenchymal Transition and Pulmonary Fibrosis via a DANCR/AUF-1/FOXO3 Regulatory Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeibin Qian et. al.PubMed 32010478
- Cancer biology & therapy 21:81-942019Diminished S-adenosylmethionine biosynthesis and its metabolism in a model of hepatocellular carcinoma is recuperated by an adenosine derivative.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaría Guadalupe Lozano-Rosas,Enrique Chávez,Gabriela Velasco-Loyden,Mariana Domínguez-López,Lidia Martínez-Pérez,Victoria Chagoya De SánchezPubMed 31552788
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human HNRNPD. The exact immunogen used to generate this antibody is proprietary information. Database link Q14103
Reactivity data
Select an application| IP | ELISA | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Expected | Expected | Tested | Tested | Tested |
| Mouse | Expected | Expected | Predicted | Predicted | Predicted |
| Rat | Expected | Expected | Predicted | Predicted | Predicted |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. May play a role in the regulation of the rhythmic expression of circadian clock core genes. Directly binds to the 3'UTR of CRY1 mRNA and induces CRY1 rhythmic translation. May also be involved in the regulation of PER2 translation.
Alternative names
AUF1, HNRPD, HNRNPD, Heterogeneous nuclear ribonucleoprotein D0, hnRNP D0, AU-rich element RNA-binding protein 1
Recommended products
Rabbit Polyclonal hnRNP D/AUF1 antibody. Suitable for IP, ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 27 publications. Immunogen corresponding to Synthetic Peptide within Human HNRNPD.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human HNRNPD. The exact immunogen used to generate this antibody is proprietary information. Database link Q14103
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The hnRNP D/AUF1 protein also known as heterogeneous nuclear ribonucleoprotein D has a molecular mass of approximately 37-40 kDa. It plays a significant role in the cellular machinery by binding to RNA molecules mainly impacting mRNA stability and turnover. The protein is expressed ubiquitously in various tissues throughout the body indicating its importance in numerous cellular functions. It is one of the hnRNP family members involved in the post-transcriptional regulation of gene expression.
- Biological function summary
HnRNP D/AUF1 regulates the degradation of mRNA by binding to AU-rich elements (AREs) found in the 3' untranslated region of many genes. It is an integral part of RNA-protein complexes that are important for controlling the half-life of mRNAs. Besides mRNA decay hnRNP D/AUF1 participates in other processes like mRNA splicing and transport. Its interactions within the ribonucleoprotein complexes highlight its versatile role in RNA metabolism.
- Pathways
HnRNP D/AUF1 operates within important biological processes such as the mRNA decay pathway and the stress response pathway. It interacts closely with other proteins involved in mRNA decay such as tristetraprolin and members of the poly-A binding protein family. hnRNP D/AUF1 influences the decay rate of different transcripts contributing to cellular responses to environmental stimuli and maintaining homeostasis.
- Associated diseases and disorders
HnRNP D/AUF1 links to inflammatory conditions and some types of cancer. Altered expression levels of AUF1 have been associated with chronic inflammation where its role in mRNA stability impacts the expression of cytokines. In cancer dysregulation of AUF1 can lead to incorrect mRNA turnover of tumor suppressors or oncogenes linking it to proteins like p53 in the context of tumorigenesis. These associations make hnRNP D/AUF1 a potential target for therapeutic interventions in related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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4 product images
Western blot - Anti-hnRNP D/AUF1 antibody (ab61193)
All lanes: Western blot - Anti-hnRNP D/AUF1 antibody (ab61193) at 1/500 dilution
Lane 1: 293 cell extract
Lane 2: 293 cell extract with immunizing peptide
Predicted band size: 38 kDa
Observed band size: 38 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP D/AUF1 antibody (ab61193)
Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue using ab61193 at 1/50-1/100 dilution. Samples were treated -/+ peptide.
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP D/AUF1 antibody (ab61193)
ICC/IF image of ab61193 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61193, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-hnRNP D/AUF1 antibody (ab61193)
hnRNP D/AUF1 western blot using anti-hnRNP D/AUF1 antibody ab61193. Publication image and figure legend from Masuda, K., Marasa, B., et al., 2009, Aging (Albany NY), PubMed 20157551.
ab61193 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab61193 please see the product overview.
TTR-RBP expression in WI-38 human diploid fibroblasts (HDFs). WI-38 HDFs were cultured for extended population doublings (pdls), until they reached senescence at ~pdl 52. The abundance of TTR-RBPs HuR, AUF1 (all four isoforms indicated), TIA-1, and TTP was assessed by Western blot analysis. GAPDH signals were included as a loading control.
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