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Rabbit Recombinant Monoclonal hnRNP G antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 6 publications.


Images

Western blot - Anti-hnRNP G antibody [EPR16038] (AB190352), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G antibody [EPR16038] (AB190352), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G antibody [EPR16038] (AB190352), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G antibody [EPR16038] (AB190352), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G antibody [EPR16038] (AB190352), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Tested
Rat
Tested
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000 - 1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000 - 1/2000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single-stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Also plays a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.

Alternative names

HNRPG, RBMXP1, RBMX, Glycoprotein p43, Heterogeneous nuclear ribonucleoprotein G, hnRNP G

Recommended products

Rabbit Recombinant Monoclonal hnRNP G antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 6 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16038
Purification technique
Affinity purification Protein A
Specificity

The immunogen used for this product shares 92% homology with human RbmxL1, 100% homology with mouse RbmxL1 and 85% homology with mouse RbmxL2. Cross-reactivity with these proteins have not been confirmed experimentally.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Heterogeneous nuclear ribonucleoprotein G (hnRNP G) also referred to as RBMX is a protein involved in RNA binding and processing. It is a member of the hnRNP protein family recognized for its role in managing the intricate processes of RNA metabolism. hnRNP G has a mass of approximately 43 kDa and is widely expressed in various tissues. The protein typically resides in the nucleus but it can shuttle between the nucleus and cytoplasm a common feature in the hnRNP family aiding in its diverse functionality.

Biological function summary

HnRNP G influences mRNA splicing stability and transport. It does not operate in isolation; instead it is part of ribonucleoprotein complexes that include other hnRNPs that work collectively. This protein facilitates alternative splicing events contributing to the generation of different protein isoforms that are essential for cellular diversity and adaptation. The ability of hnRNP G to bind to RNA and participate in these cellular events highlights its significance in regulatory processes.

Pathways

HnRNP G plays a role in the regulation of gene expression. It is an important player in the alternative splicing pathway affecting the diversity of mRNA products. hnRNP G interacts with the splicing machinery and can influence splice site selection. This protein is also associated with the DNA damage response pathway where it interacts with other proteins such as BRCA1 particularly under stress conditions that require enhanced DNA repair mechanisms.

Associated diseases and disorders

HnRNP G has connections to certain types of cancer and neurological disorders. Altered expression of hnRNP G is observed in breast cancer suggesting its role in tumor progression and gene regulation disruptions. Additionally changes in hnRNP G activity have been associated with neurodegenerative diseases like spinal muscular atrophy. In these contexts hnRNP G interfaces with proteins such as SMN1 linking its expression and function to disease outcomes and offering potential avenues for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-hnRNP G antibody [EPR16038] (ab190352), expandable thumbnail

    Western blot - Anti-hnRNP G antibody [EPR16038] (ab190352)

    All lanes: Western blot - Anti-hnRNP G antibody [EPR16038] (ab190352) at 1/1000 dilution

    Lane 1: HeLa lysate at 20 µg

    Lane 2: Jurkat lysate at 20 µg

    Lane 3: K562 lysate at 20 µg

    Lane 4: A431 lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G antibody [EPR16038] (ab190352), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G antibody [EPR16038] (ab190352)

    Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling hnRNP G with ab190352 at 1/250 dilution. Counter stain Hematoxylin. Negative control also shown lower left.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G antibody [EPR16038] (ab190352), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G antibody [EPR16038] (ab190352)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling hnRNP G with ab190352 at a 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution (Red). Negative controls and DAPI staining also shown.

  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G antibody [EPR16038] (ab190352), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G antibody [EPR16038] (ab190352)

    Immunofluorescent analysis of 4% paraformaldehyde fixed A431 cells labeling hnRNP G with ab190352 at a 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution (Red). Negative controls and DAPI staining also shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G antibody [EPR16038] (ab190352), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G antibody [EPR16038] (ab190352)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling hnRNP G with ab190352 at 1/250 dilution. Counter stain Hematoxylin. Negative control also shown lower left.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-hnRNP G antibody [EPR16038] (ab190352), expandable thumbnail

    Western blot - Anti-hnRNP G antibody [EPR16038] (ab190352)

    All lanes: Western blot - Anti-hnRNP G antibody [EPR16038] (ab190352) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Rat brain lysate at 10 µg

    Lane 3: C6 lysate at 10 µg

    Lane 4: NIH/3T3 lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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