AB190352

Anti-hnRNP G antibody [EPR16038]

Name: Anti-hnRNP G antibody [EPR16038] (ab190352) | Abcam
Brand: Abcam Limited
SKU: AB190352
Price: 355 USD
Availability: InStock
Rating: 4 (3 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(3 Reviews)

(7 Publications)

Rabbit Recombinant Monoclonal hnRNP G antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 7 publications.

View Alternative Names

HNRPG, RBMXP1, RBMX, Glycoprotein p43, Heterogeneous nuclear ribonucleoprotein G, hnRNP G

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G antibody [EPR16038] (AB190352)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G antibody [EPR16038] (AB190352)

Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling hnRNP G with ab190352 at a 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution (Red). Negative controls and DAPI staining also shown.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G antibody [EPR16038] (AB190352)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G antibody [EPR16038] (AB190352)

Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling hnRNP G with ab190352 at 1/250 dilution. Counter stain Hematoxylin. Negative control also shown lower left.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G antibody [EPR16038] (AB190352)

Immunofluorescent analysis of 4% paraformaldehyde fixed A431 cells labeling hnRNP G with ab190352 at a 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution (Red). Negative controls and DAPI staining also shown.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G antibody [EPR16038] (AB190352)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling hnRNP G with ab190352 at 1/250 dilution. Counter stain Hematoxylin. Negative control also shown lower left.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-hnRNP G antibody [EPR16038] (AB190352)

All lanes:

Western blot - Anti-hnRNP G antibody [EPR16038] (ab190352) at 1/1000 dilution

Lane 1:

HeLa lysate at 20 µg

Lane 2:

Jurkat lysate at 20 µg

Lane 3:

K562 lysate at 20 µg

Lane 4:

A431 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 42 kDa

false

Supplier Data

Western blot - Anti-hnRNP G antibody [EPR16038] (AB190352)

All lanes:

Western blot - Anti-hnRNP G antibody [EPR16038] (ab190352) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Lane 3:

C6 lysate at 10 µg

Lane 4:

NIH/3T3 lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

421 Alexa Fluor® 405

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed
421 Alexa Fluor® 405

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405)
421 Alexa Fluor® 405

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed
603 Alexa Fluor® 568

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16038

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 92% homology with human RbmxL1, 100% homology with mouse RbmxL1 and 85% homology with mouse RbmxL2. Cross-reactivity with these proteins have not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heterogeneous nuclear ribonucleoprotein G (hnRNP G) also referred to as RBMX is a protein involved in RNA binding and processing. It is a member of the hnRNP protein family recognized for its role in managing the intricate processes of RNA metabolism. hnRNP G has a mass of approximately 43 kDa and is widely expressed in various tissues. The protein typically resides in the nucleus but it can shuttle between the nucleus and cytoplasm a common feature in the hnRNP family aiding in its diverse functionality.

Biological function summary

HnRNP G influences mRNA splicing stability and transport. It does not operate in isolation; instead it is part of ribonucleoprotein complexes that include other hnRNPs that work collectively. This protein facilitates alternative splicing events contributing to the generation of different protein isoforms that are essential for cellular diversity and adaptation. The ability of hnRNP G to bind to RNA and participate in these cellular events highlights its significance in regulatory processes.

Pathways

HnRNP G plays a role in the regulation of gene expression. It is an important player in the alternative splicing pathway affecting the diversity of mRNA products. hnRNP G interacts with the splicing machinery and can influence splice site selection. This protein is also associated with the DNA damage response pathway where it interacts with other proteins such as BRCA1 particularly under stress conditions that require enhanced DNA repair mechanisms.

HnRNP G has connections to certain types of cancer and neurological disorders. Altered expression of hnRNP G is observed in breast cancer suggesting its role in tumor progression and gene regulation disruptions. Additionally changes in hnRNP G activity have been associated with neurodegenerative diseases like spinal muscular atrophy. In these contexts hnRNP G interfaces with proteins such as SMN1 linking its expression and function to disease outcomes and offering potential avenues for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single-stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Also plays a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.

See full target information RBMX

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cancers 17: PubMed40941025

2025

The RNA-Binding Protein RBMX Mediates the Immunosuppressive Microenvironment of Osteosarcoma by Regulating CD8T Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Qiu,Chao Pu,Chengguang Wang,Zhengxue Quan

PLoS genetics 19:e1010937 PubMed37756323

2023

RBMX involves in telomere stability maintenance by regulating TERRA expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jingfan Liu,Tian Zheng,Dandan Chen,Junjiu Huang,Yong Zhao,Wenbin Ma,Haiying Liu

Cancers 13: PubMed34638274

2021

RBMX Protein Expression in T-Cell Lymphomas Predicts Chemotherapy Response and Prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Franziska Lea Schümann,Marcus Bauer,Elisabeth Groß,Denis Terziev,Andreas Wienke,Claudia Wickenhauser,Mascha Binder,Thomas Weber

Advanced science (Weinheim, Baden-Wurttemberg, Ger 7:2002518 PubMed33173749

2020

CD63 Cancer-Associated Fibroblasts Confer Tamoxifen Resistance to Breast Cancer Cells through Exosomal miR-22.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Gao,Xiaoju Li,Cheng Zeng,Chenlin Liu,Qiang Hao,Weina Li,Kuo Zhang,Wangqian Zhang,Shuning Wang,Huadong Zhao,Dong Fan,Meng Li,Yingqi Zhang,Wei Zhang,Cun Zhang

Cell death and differentiation 27:3162-3176 PubMed32494026

2020

RBMX is required for activation of ATR on repetitive DNAs to maintain genome stability.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Zheng,Haoxian Zhou,Xiaocui Li,Di Peng,Yiding Yang,Yanru Zeng,Haiying Liu,Jian Ren,Yong Zhao

Molecular cell 76:70-81.e9 PubMed31445886

2019

Regulation of Co-transcriptional Pre-mRNA Splicing by mA through the Low-Complexity Protein hnRNPG.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine I Zhou,Hailing Shi,Ruitu Lyu,Adam C Wylder,Żaneta Matuszek,Jessica N Pan,Chuan He,Marc Parisien,Tao Pan

Methods (San Diego, Calif.) 126:105-111 PubMed28454774

2017

Identification of N-methyladenosine reader proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine I Zhou,Nian Liu,Tao Pan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-hnRNP G antibody [EPR16038]

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G antibody [EPR16038] (AB190352)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G antibody [EPR16038] (AB190352)

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G antibody [EPR16038] (AB190352)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G antibody [EPR16038] (AB190352)

Western blot - Anti-hnRNP G antibody [EPR16038] (AB190352)

Western blot - Anti-hnRNP G antibody [EPR16038] (AB190352)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise