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AB238987

Anti-hnRNP G antibody [EPR16038] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal hnRNP G antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

HNRPG, RBMXP1, RBMX, Glycoprotein p43, Heterogeneous nuclear ribonucleoprotein G, hnRNP G

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G antibody [EPR16038] - BSA and Azide free (AB238987)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G antibody [EPR16038] - BSA and Azide free (AB238987)

Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling hnRNP G with ab190352 at a 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution (Red). Negative controls and DAPI staining also shown.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190352).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G antibody [EPR16038] - BSA and Azide free (AB238987)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G antibody [EPR16038] - BSA and Azide free (AB238987)

Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling hnRNP G with ab190352 at 1/250 dilution. Counter stain Hematoxylin. Negative control also shown lower left.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190352).

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G antibody [EPR16038] - BSA and Azide free (AB238987)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G antibody [EPR16038] - BSA and Azide free (AB238987)

Immunofluorescent analysis of 4% paraformaldehyde fixed A431 cells labeling hnRNP G with ab190352 at a 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution (Red). Negative controls and DAPI staining also shown.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190352).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G antibody [EPR16038] - BSA and Azide free (AB238987)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G antibody [EPR16038] - BSA and Azide free (AB238987)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling hnRNP G with ab190352 at 1/250 dilution. Counter stain Hematoxylin. Negative control also shown lower left.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190352).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16038

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 92% homology with human RbmxL1, 100% homology with mouse RbmxL1 and 85% homology with mouse RbmxL2. Cross-reactivity with these proteins have not been confirmed experimentally.

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Primary Antibodies

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Primary Antibodies

AB109535

Anti-TDP43 antibody [EPR5810]

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab238987 is the carrier-free version of ab190352.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heterogeneous nuclear ribonucleoprotein G (hnRNP G) also referred to as RBMX is a protein involved in RNA binding and processing. It is a member of the hnRNP protein family recognized for its role in managing the intricate processes of RNA metabolism. hnRNP G has a mass of approximately 43 kDa and is widely expressed in various tissues. The protein typically resides in the nucleus but it can shuttle between the nucleus and cytoplasm a common feature in the hnRNP family aiding in its diverse functionality.
Biological function summary

HnRNP G influences mRNA splicing stability and transport. It does not operate in isolation; instead it is part of ribonucleoprotein complexes that include other hnRNPs that work collectively. This protein facilitates alternative splicing events contributing to the generation of different protein isoforms that are essential for cellular diversity and adaptation. The ability of hnRNP G to bind to RNA and participate in these cellular events highlights its significance in regulatory processes.

Pathways

HnRNP G plays a role in the regulation of gene expression. It is an important player in the alternative splicing pathway affecting the diversity of mRNA products. hnRNP G interacts with the splicing machinery and can influence splice site selection. This protein is also associated with the DNA damage response pathway where it interacts with other proteins such as BRCA1 particularly under stress conditions that require enhanced DNA repair mechanisms.

HnRNP G has connections to certain types of cancer and neurological disorders. Altered expression of hnRNP G is observed in breast cancer suggesting its role in tumor progression and gene regulation disruptions. Additionally changes in hnRNP G activity have been associated with neurodegenerative diseases like spinal muscular atrophy. In these contexts hnRNP G interfaces with proteins such as SMN1 linking its expression and function to disease outcomes and offering potential avenues for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single-stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Also plays a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.
See full target information RBMX
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of human genetics 110:1394-1413 PubMed37467750

2023

Monoallelic variation in DHX9, the gene encoding the DExH-box helicase DHX9, underlies neurodevelopment disorders and Charcot-Marie-Tooth disease.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel G Calame,Tianyu Guo,Chen Wang,Lillian Garrett,Angad Jolly,Moez Dawood,Alina Kurolap,Noa Zunz Henig,Jawid M Fatih,Isabella Herman,Haowei Du,Tadahiro Mitani,Lore Becker,Birgit Rathkolb,Raffaele Gerlini,Claudia Seisenberger,Susan Marschall,Jill V Hunter,Amanda Gerard,Alexis Heidlebaugh,Thomas Challman,Rebecca C Spillmann,Shalini N Jhangiani,Zeynep Coban-Akdemir,Seema Lalani,Lingxiao Liu,Anya Revah-Politi,Alejandro Iglesias,Edwin Guzman,Evan Baugh,Nathalie Boddaert,Sophie Rondeau,Clothide Ormieres,Giulia Barcia,Queenie K G Tan,Isabelle Thiffault,Tomi Pastinen,Kazim Sheikh,Suur Biliciler,Davide Mei,Federico Melani,Vandana Shashi,Yuval Yaron,Mary Steele,Emma Wakeling,Elsebet Østergaard,Lusine Nazaryan-Petersen,Francisca Millan,Teresa Santiago-Sim,Julien Thevenon,Ange-Line Bruel,Christel Thauvin-Robinet,Denny Popp,Konrad Platzer,Pawel Gawlinski,Wojciech Wiszniewski,Dana Marafi,Davut Pehlivan,Jennifer E Posey,Richard A Gibbs,Valerie Gailus-Durner,Renzo Guerrini,Helmut Fuchs,Martin Hrabě de Angelis,Sabine M Hölter,Hoi-Hung Cheung,Shen Gu,James R Lupski
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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