AB178429

Anti-hnRNP G-T antibody [EPR12593-14]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal hnRNP G-T antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HNRNPGT, RBMXL2, Testis-specific heterogeneous nuclear ribonucleoprotein G-T, hnRNP G-T

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G-T antibody [EPR12593-14] (AB178429)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G-T antibody [EPR12593-14] (AB178429)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling hnRNP G-T with ab178429 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-hnRNP G-T antibody [EPR12593-14] (AB178429)

All lanes:

Western blot - Anti-hnRNP G-T antibody [EPR12593-14] (ab178429) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 10 µg

Lane 2:

Human seminoma lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody

Predicted band size: 43 kDa

true

Carrier free

Anti-hnRNP G-T antibody [EPR12593-14] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12593-14

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.35% Sodium citrate, 0.17% Sodium chloride, 0.05% BSA, 0.03% EDTA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heterogeneous nuclear ribonucleoprotein G-T (hnRNP G-T) also known as RBMX2 is a member of the hnRNP family. This protein has a molecular mass of approximately 41 kDa. Mechanically hnRNP G-T functions in RNA binding and splicing playing roles in the regulation of mRNA metabolism. Expression of hnRNP G-T is found in various human tissues with notable presence in the testis implicating potential roles in testicular functions and spermatogenesis.

Biological function summary

HnRNP G-T participates in post-transcriptional gene regulation. It forms complexes with RNA molecules affecting the outcome of splicing events. Such involvement influences the diversity and stability of mRNA which can impact protein translation. In addition to its RNA-binding capacity hnRNP G-T's function extends to modulating mRNA export from the nucleus to the cytoplasm affecting protein synthesis.

Pathways

HnRNP G-T integrates within the splicing machinery affecting the spliceosome pathway. It ensures accurate splicing decisions that are essential for producing functional proteins. hnRNP G-T shows interactions with other splicing-related proteins like SR proteins which enhance or repress splicing decisions based on cellular context. Its involvement also extends to the mRNA surveillance pathway maintaining RNA quality control processes that detect and degrade faulty mRNA transcripts.

Deficiencies or aberrations in hnRNP G-T activity have associations with male infertility and certain cancers such as testicular and prostate cancer. Its interaction with proteins like BRCA1 highlights its potential impacts on genomic stability and cell-cycle regulation pathways. Differences in hnRNP G-T function or expression contribute to improper splicing events leading to pathological conditions that disrupt normal cellular functions and enhance disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein involved in alternative splicing in the testis during spermatogenesis.

See full target information RBMXL2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in endocrinology 12:703167 PubMed34925225

2021

The miR-4739/DLX3 Axis Modulates Bone Marrow-Derived Mesenchymal Stem Cell (BMSC) Osteogenesis Affecting Osteoporosis Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ding Li,Qi Yuan,Liang Xiong,Aoyu Li,Yu Xia

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-hnRNP G-T antibody [EPR12593-14]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G-T antibody [EPR12593-14] (AB178429)

Western blot - Anti-hnRNP G-T antibody [EPR12593-14] (AB178429)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise